A novel strategy to identify maternal and paternal inheritance in the mouse

Römer, Irmgard; Jungblut, Peter R.; Reik, Wolf; Otto, Albrecht; Klose, Joachim

doi:10.1002/elps.11501601137

Cited by 6 publications

(3 citation statements)

References 35 publications

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“…The present study is part of a long-term endeavor aimed at the identification of murine strain-specific protein variants, which reveal a mode of inheritance compatible with the concept of genomic imprinting, i.e., a maternal or paternal transmission [33,34]. Silver-stained 2-DE patterns of cytosolic liver proteins were investigated in 8 parental mice (2 mice of each gender) of the 2 subspecies M. musculus musculus (inbred strain: Calcinato B) and M. musculus domesticus (inbred strain: Aarhus) and in their offspring (25 F 1 hybrids: 14 females, 11 males).…”

Section: Resultsmentioning

confidence: 99%

Selenium‐binding protein 2, the major hepatic target for acetaminophen, shows sex differences in protein abundance

Mattow¹,

Demuth²,

Haeselbarth³

et al. 2006

Electrophoresis

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Liver samples from female and male mice of two subspecies, Mus musculus musculus and Mus musculus domesticus, were investigated by a combination of 2-DE and MALDI-MS. The image analysis of the generated 2-DE patterns revealed several protein spots with significant differences in intensity/abundance between the sexes. Seven protein spots, which were prominent in 2-DE patterns of male mice, but which showed very low intensities in females, were identified as selenium-binding protein 2 (SBP2) also known as 56-kDa acetaminophen-binding protein. Edman degradation indicated that at least three of these protein spots represent N-terminally truncated SBP2 variants. Furthermore, it was shown that the observed differences in SBP2 abundance correlate with sex differences in transcription of the gene encoding SBP2, selenbp2, as revealed by RT-PCR and restriction digest as well as sequence analysis of the products. Since SBP2 has been described as the major target for acetaminophen in mouse liver cytosol, these findings are discussed with respect to their possible relevance for sex differences in acetaminophen-mediated toxicity, which have been described in a variety of mammals including mice and rats.

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Section: Resultsmentioning

confidence: 99%

Selenium‐binding protein 2, the major hepatic target for acetaminophen, shows sex differences in protein abundance

Mattow¹,

Demuth²,

Haeselbarth³

et al. 2006

Electrophoresis

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“…Proteins separated by 2-DE were blotted onto PVDF membranes (Millipore, Bedford, MA, USA), stained with Coomassie Brilliant Blue, and spots of interest were microsequenced and identified as described before [8,9]. Electrotransfer of the proteins on Hybond C nitrocellulose membranes (Amersham, Buckinghamshire, UK) was done under semidry blotting conditions [8] using the continous blotting buffer system described by Towbin et al [10] for both the covalent oxidative binding of DIG and the lectin affinoblotting. Glycoprotein staining was performed essentially following the standard procedure described by the manual of the DIG glycan detection kit (Boehringer, Mannheim, Germany).…”

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confidence: 99%

“…Glycoprotein staining was performed essentially following the standard procedure described by the manual of the DIG glycan detection kit (Boehringer, Mannheim, Germany). The procedure developed for blots of SDS-gels was adapted to 2-DE blots by repeating the blocking, washing, and incubation steps as previously published [8]. Dotblots of 0.5 mg bovine serum albumin and transferrin were used as positive controls, 0.5 mg deglycosylated creatinase as negative controls.…”

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Analysis of mouse β-haptoglobin chain by lectin affinoblotting detection

Roemer

Vogel

Otto³

et al. 2001

Electrophoresis

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Two different human mammary carcinoma cell lines were xenotransplanted into nude mice. Serum samples were obtained prior to and after transplantation and investigated by two-dimensional electrophoresis (2-DE). By comparison of these silver-stained patterns additional protein spots were detected resulting either from proteins secreted or shed by the tumor itself or from mouse proteins induced by the tumor or the transplantation procedure. One group of spots detectable in post-transplantation serum as well as in control serum after mock-transplantation but not in pretransplantation serum was microsequenced and identified as mouse beta-haptoglobin. The carbohydrate structures of beta-haptoglobin were characterized by two different immunochemical glycoprotein staining procedures to detect differential terminal glycan modifications.

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Protein identification from 2-DE gels by MALDI mass spectrometry

Jungblut

Thiede

1997

Mass Spectrom. Rev.

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167

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Two‐dimensional electrophoresis (2‐DE) allows the separation of proteins at the level of protein species, which are defined by their chemical structure. Each chemical modification leads to a new protein species. Prerequisites for investigations of protein species are reproducible sample preparation, large gels (20 × 30 cm and larger), high sensitive detection methods, automated evaluation of gels, and sensitive methods for identification of 2‐DE‐separated protein species. MALDI mass spectrometry with its sensitivity in the fmol range fits with the sensitivity of protein detection on 2‐DE gels. Protein mass determination, peptide mass mapping, post‐source decay sequencing, and ladder sequencing by MALDI‐MS in combination with genome databases have the potential for complete structural investigation on the protein species level. The discrimination of 19 crystalline species of mouse eye lens and of 54 human heart heatshock protein 27 species shows the capacity of the combination of 2‐DE with MALDI‐MS. © 1997 John Wiley & Sons, Inc., Mass Spectrom Rev 16(3), 145–162, 1997

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A novel strategy to identify maternal and paternal inheritance in the mouse

Cited by 6 publications

References 35 publications

Selenium‐binding protein 2, the major hepatic target for acetaminophen, shows sex differences in protein abundance

Selenium‐binding protein 2, the major hepatic target for acetaminophen, shows sex differences in protein abundance

Analysis of mouse β-haptoglobin chain by lectin affinoblotting detection

Protein identification from 2-DE gels by MALDI mass spectrometry

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