Two-Dimensional Electrophoresis of Pseudocholinesterase Components in Normal Human Serum

Silent cholinesterase gene: variations in the properties of serum enzyme in apparent homozygotes

“…Numbering from the anodal end, band 5 is the most intense, accounting for the major portion of the total activity. The bands cathodal to 5 are poorly resolved (22), particularly on acrylamide, are variable in number (23), and are known to be affected by storage (24). Our numbering system and results are identical to those of LaMotta, McComb, and Wetstone (25).…”

Increased plasma cholinesterase activity and succinylcholine resistance: a genetic variant.

“…Acrylamide gel (Canalco) was prepared with Tris-glycine buffer (pH 8.6). The material was stained for cholinesterase with a-naphthylacetate as substrate and fast blue B diazo coupler (21).…”

Cholinesterase: phenotyping and genotyping

“…In some individuals, enzymes with a higher activity than normal have been found which demonstrate electrophoretic variation from`usual' but the same inhibition characteristics. 12 One of these was designated the C5 variant and was thought to arise from a second BChE gene locus that was called E 2 , 13 although no molecular evidence for this second locus has been found to date (see below). The enzyme types attributed via biochemical analysis and inhibition numbers were phenotypes from which a genotype (as de¢ned by terms such as E 1 u E 1 u and E 1 a E 1 a ) was inferred.…”