Two‐dimensional electrophoresis of prostate‐specific antigen in sera of men with prostate cancer or benign prostate hyperplasia

Free PSA forms in prostatic tissue and sera of prostate cancer patients: Analysis by 2-DE and western blotting of immunopurified samples

“…In agreement with reports of other authors [11][12][13], the lower MW fPSA spots observed (J and M) could represent nicked fPSA isoforms, but no further investigations were done in this study on these low MW fPSA spots.…”

“…All the samples contain the spots F1 to F4 but the isoforms E and G are only detected in the serum samples. The analysis of fPSA forms from PCa and NPCa sera by 2-DE and western blotting has already been described [11][12][13]. Figs.…”

“…proPSA, BPH-associated PSA, nicked PSA) have also been carried out and have shown promise to be useful for diagnostic purposes [8][9][10]. Moreover the 2-DE pattern of fPSA forms from PCa and BPH sera have recently been characterized in detail, and it has been demonstrated that such analysis could improve both sensitivity and specificity in prostate cancer diagnostics [11][12][13][14]. In addition the 2-DE pattern of fPSA from seminal plasma and conditioned media from LNCaP cells have also been analyzed to a certain extent [15], and the heterogeneity of fPSA in seven different commercially available fPSA samples from seminal plasma have been analyzed by capillary electrophoresis [16].…”

Proteomic detection of prostate-specific antigen using a serum fractionation procedure: potential implication for new low-abundance cancer biomarkers detection

“…As previously described, using classical analytical 2-DE gels, it is not possible to observe free and cleaved forms of PSA in whole serum (Figs. 3A and C) [13]. Arrows point to the two spots that diVered in quantity between fraction 5 (Fig.…”

Identification of proteins in human prostate tumor material by two-dimensional gel electrophoresis and mass spectrometry

“…Important tools in prostate cancer diagnosis are serum markers such as prostate-specific antigen (PSA) and prostatic acid phosphatase (PAP) [2,3]. However, diagnosis based on PSA levels in the range of 4 -20 ng PSA/ml serum does not differentiate neoplasia from hyperplasia [2,4], and when high PSA levels are detected, the tumor often displays extracapsular progression, which means poor prognosis [5]. Therefore, efforts should be made to find sensitive and specific diagnostic markers which can discriminate between tumor subtypes.…”