Free PSA forms in prostatic tissue and sera of prostate cancer patients: Analysis by 2-DE and western blotting of immunopurified samples

Tabarés, Glòria; Jung, Klaus; Reiche, Janett; Stephan, Carsten; Lein, Michael; Peracaula, Rosa; Llorens, Rafael de; Hoesel, Wolfgang

doi:10.1016/j.clinbiochem.2006.12.006

Cited by 26 publications

(28 citation statements)

References 36 publications

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“…Other authors have suggested that the presence of proPSA forms are constituents of the total free PSA subforms detected in the serum by 2-DE. Tabares et al (2007) reported the presence of proPSA forms in some of the free PSA serum 2-DE subforms. However, in our PSA 2-DE subforms from serum, the contribution from proPSA would be quite small as the majority of the PSA analyzed corresponded to active PSA previously bound to ACT.…”

Section: Characterization Of Psa 2-de Subformsmentioning

confidence: 99%

“…An increase or decrease in the content of this monosaccharide on a PSA N-glycan chain can modify the PSA's isoelectric point (pI). Two-dimensional gel electrophoresis has been used to evaluate sialic acid variations in PSA from different sources (Charrier et al, 1999;Isono et al, 2002;Jung et al, 2004;Tabares et al, 2007). Most of these studies have focused on fPSA 2-DE subforms, which represent only 10-30% of the total serum PSA.…”

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confidence: 99%

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Glycan Characterization of PSA 2-DE Subforms from Serum and Seminal Plasma

Sarrats

Saldova²,

Comet³

et al. 2010

OMICS: A Journal of Integrative Biology

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Prostate-specific antigen (PSA) two-dimensional electrophoresis (2-DE) subforms (F1-F5) have been described to be altered in prostate cancer (PCa) compared to benign prostatic hyperplasia (BPH). To understand their molecular differences, characterization of these subforms from PCa serum and seminal plasma, namely, at the glycan level, was performed. PSA 2-DE subforms from two serum PCa samples and seminal plasma were analyzed by N-glycan sequencing using high-performance liquid chromatography (HPLC) combined with exoglycosidase array digestions and by mass spectrometry. F1, F2, and F3 subforms showed the same N-glycan pattern, which contained higher levels of sialic acid than the F4 subform, whereas the F5 subform was unglycosylated. When comparing PSA subforms from PCa with seminal plasma, a decrease in sialylation was observed. Furthermore, the analysis of F3, the more abundant PSA subform, showed a higher proportion of alpha 2-3 sialic acid and a decrease in core fucosylated glycans in the PCa sample. These N-glycan changes in PCa PSA subforms highlight the importance of glycosylation as an indicator of PCa disease.

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Section: Characterization Of Psa 2-de Subformsmentioning

confidence: 99%

mentioning

confidence: 99%

Glycan Characterization of PSA 2-DE Subforms from Serum and Seminal Plasma

Sarrats

Saldova²,

Comet³

et al. 2010

OMICS: A Journal of Integrative Biology

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“…For example, prostate-specific antigen (PSA) is a prostate cancer biomarker and a glycoprotein. The glycosylation pattern of PSA from cancer is known to be different from that of normal tissue [53,[111][112][113][114][115][116][117][118][119][120][121]. Tools that allow for detection and differentiation of the various glycoforms of a given glycoprotein will be very useful.…”

Section: Boronic-acid-based Chemosensorsmentioning

confidence: 99%

Covalent Interactions in Chemosensor Design

2011

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“…Certain PSA values are definitive and accurate, and the test has proved both popular and useful. However, many patients present with only slightly elevated PSA values, and for this group the test does not provide a differential diagnosis between prostate cancer and benign prostate hyperplasia (BPH) [5]. Besides BPH and prostate cancer themselves, other factors can influence PSA levels; they are increased, for example, by any handling of the prostate (prostate biopsy, digital rectal examination), infections, chronic or acute (prostatitis), recent ejaculation and prolonged cycling, which can cause temporary damage to the microenvironment of the gland [4].…”

Section: Introductionmentioning

confidence: 99%