Two-dimensional electrophoresis and characterization of antigens from

Fonseca, Cristiane A. da; Jesuino, Rosália Santos Amorim; Felipe, Maria Sueli Soares; Cunha, Daniela A.; Brito, Wesley A.; Soares, C. M. A.

doi:10.1016/s1286-4579(01)01409-5

Cited by 71 publications

(77 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…From Figure 3 we can see that the active compounds present negative values for PC 1 while the inactive compounds present positive values for PC 1 .…”

Section: Pca and Hca Analysesmentioning

confidence: 94%

“…The microorganism is the etiological agent of paracoccioidomycosis (PCM), a systemic disease with a high incidence among the rural population of Latin America. 1 This disease is usually chronic and involves several organs and systems with predominance in the lungs which are considered as the primary site of the infection. 2 Effective treatment regimens are available to control the infectious process and most patients (60-80%) develop fibrotic sequelae that may severely hamper respiratory functions and limit the patient well-being.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A study of neolignan compounds with biological activity against Paracoccidioides brasiliensis by using quantum chemical and chemometric methods

Camargo¹,

Honório²,

Mercadante³

et al. 2003

J. Braz. Chem. Soc.

View full text Add to dashboard Cite

Métodos quimiométricos (estatísticos) são empregados para classificar um conjunto de compostos derivados de neolignanas com atividade biológica contra a Paracoccidioides brasiliensis. O método AM1 (Austin Model 1) foi utilizado para calcular um conjunto de descritores moleculares (propriedades) para os compostos em estudo. A seguir, os descritores foram analisados utilizando os seguintes métodos de reconhecimento de padrões: Análise de Componentes Principais (PCA), Análise Hierárquica de Agrupamentos (HCA) e o método de K-vizinhos mais próximos (KNN). Os métodos PCA e HCA mostraram-se bastante eficientes para classificação dos compostos estudados em dois grupos (ativos e inativos). Três descritores moleculares foram responsáveis pela separação entre os compostos ativos e inativos: energia do orbital molecular mais alto ocupado (E HOMO ), ordem de ligação entre os átomos C 1' -R 7 (L 14 ) e ordem de ligação entre os átomos C 5' -R 6 (L 22 ). Como as variáveis responsáveis pela separação entre compostos ativos e inativos são descritores eletrônicos, conclui-se que efeitos eletrônicos podem desempenhar um importante papel na interação entre receptor biológico e compostos derivados de neolignanas com atividade contra a Paracoccidioides brasiliensis.Chemometric (statistical) methods are employed to classify a set of neolignan compounds with activity against Paracoccidioides brasiliensis. The AM1 (Austin Model 1) method was used to calculate a set of molecular descriptors (properties) of the neolignan compounds under study. The descriptors were further analyzed using Principal Components Analysis (PCA), Hierarchical Cluster Analysis (HCA) and K-Nearest Neighbor (KNN) chemometric methods. The PCA and HCA methods were quite efficient to classify the compounds into two groups (active and inactive) and three descriptors were found to be important in the classification: the highest occupied molecular orbital energy (E HOMO ), the bond order between C 1' -R 7 atoms (L 14 ) and the bond order between C 5' -R 6 atoms (L 22 ). The three variables responsible for the separation between active and inactive compounds are all electronic descriptors, therefore we can conclude that electronic effects should have an important role when one is trying to understand the activity of neolignan compounds against Paracoccidioides brasiliensis.Keywords: neolignans, Paracoccidioides brasiliensis, AM1, PCA, HCA, KNN IntroductionParacoccidioides brasiliensis is a human pathogenic fungus that constitutes a major medical problem in Latin America. The microorganism is the etiological agent of paracoccioidomycosis (PCM), a systemic disease with a high incidence among the rural population of Latin America. 1 This disease is usually chronic and involves several organs and systems with predominance in the lungs which are considered as the primary site of the infection. 2 Effective treatment regimens are available to control the infectious process and most patients (60-80%) develop fibrotic sequelae that may severely hamper respiratory functions and l...

show abstract

“…From Figure 3 we can see that the active compounds present negative values for PC 1 while the inactive compounds present positive values for PC 1 .…”

Section: Pca and Hca Analysesmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

A study of neolignan compounds with biological activity against Paracoccidioides brasiliensis by using quantum chemical and chemometric methods

Camargo¹,

Honório²,

Mercadante³

et al. 2003

J. Braz. Chem. Soc.

View full text Add to dashboard Cite

show abstract

“…Glyceraldehyde-3-phosphate-dehydrogenase (GAPDH) is induced in Pb01 obtained from infected mouse liver and in yeast cells incubated in blood (Bailão et al 2006). The GAPDH of P. brasiliensis reacts with the serum of PCM patients (Fonseca et al 2001, Barbosa et al 2004 and is located at the fungal cell wall. This protein is a P. brasiliensis adhesin that binds components of the extracellular matrix and is able to mediate the adherence and internalization of P. brasiliensis to in vitro-cultured cells, suggesting its involvement in the pathogenesis of the fungus (Barbosa et al 2006).…”

Section: Niche-regulation Of Genes Related To Cell Rescue and Virulencementioning

confidence: 99%

Preferential transcription of Paracoccidioides brasiliensis genes: host niche and time-dependent expression

Pereira

Bailão

Parente

et al. 2009

Mem. Inst. Oswaldo Cruz

View full text Add to dashboard Cite

show abstract

“…The P. brasiliensis isolate Pb01 (ATCC, MYA 826) has been previously investigated by our group (Pereira et al, 2000;Fonseca et al, 2001). It was cultivated in Fava-Neto's medium (Fava-Neto, 1961) at 23…”

Section: Microrganism and Growth Conditionsmentioning

confidence: 99%

“…The cells were frozen in the same buffer containing the protease inhibitors: 50 µg/ml N-α-ptosyl-L-lysine (TLCK) and N-tosyl-L-phenylalanine (TPCK), 5 mM iodoacetamide, 1 mM EDTA, 20 mM leupeptin and 4-chloromercuribenzoic acid (PCMB). The cells were disrupted by mechanical maceration, and the proteins were prepared as described by Fonseca et al (2001). Debris was removed by centrifugation at 12 000× g for 5 min and the proteins were precipitated by 10% (w/v) trichloroacetic acid (TCA) addition.…”

Section: Protein Electrophoresis and Immunodetection Of P Brasiliensmentioning

confidence: 99%

Characterization of a chaperone ClpB homologue of Paracoccidioides brasiliensis

Jesuino

Azevedo

Felipe

et al. 2002

Yeast

View full text Add to dashboard Cite

show abstract

Two-dimensional electrophoresis and characterization of antigens from

Cited by 71 publications

References 47 publications

A study of neolignan compounds with biological activity against Paracoccidioides brasiliensis by using quantum chemical and chemometric methods

A study of neolignan compounds with biological activity against Paracoccidioides brasiliensis by using quantum chemical and chemometric methods

Preferential transcription of Paracoccidioides brasiliensis genes: host niche and time-dependent expression

Characterization of a chaperone ClpB homologue of Paracoccidioides brasiliensis

Contact Info

Product

Resources

About