Preferential transcription of Paracoccidioides brasiliensis genes: host niche and time-dependent expression

Pereira, Maristela; Bailão, Alexandre Melo; Parente, Juliana Alves; Borges, Clayton Luiz; Salem-Izacc, Sílvia Maria; Soares, Célia Maria de Almeida

doi:10.1590/s0074-02762009000300015

Cited by 8 publications

(7 citation statements)

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“…utilizes propionate as a carbon source Paracoccidioides spp. transcripts/enzymes related to propionate metabolism had been identified in infectionmimicking conditions (Bailao et al 2006;Bailao et al 2007;Lima et al 2014;Parente et al 2011;Pereira et al 2009;Rezende et al 2011). Therefore, fungal ability to grow and survive in the presence of propionate was analyzed.…”

Section: Resultsmentioning

confidence: 99%

“…Nutrient limitation is a common strategy employed by the host to hamper fungal survival. Furthermore, each host niche encountered by a microorganism may provide a different type of carbon source (Brock 2009;Pereira et al 2009). Therefore, the ability to acquire nutrients from the host and a metabolic flexibility are central pieces of fungal virulence arsenal.…”

Section: Introductionmentioning

confidence: 99%

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Propionate metabolism in a human pathogenic fungus: proteomic and biochemical analyses

et al. 2020

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Fungi of the complex Paracoccidioides spp. are thermodimorphic organisms that cause Paracoccidioidomycosis, one of the most prevalent mycoses in Latin America. These fungi present metabolic mechanisms that contribute to the fungal survival in host tissues. Paracoccidioides lutzii activates glycolysis and fermentation while inactivates aerobic metabolism in iron deprivation, a condition found during infection. In lungs Paracoccidioides brasiliensis face a glucose poor environment and relies on the beta-oxidation to support energy requirement. During mycelium to yeast transition P. lutzii cells up-regulate transcripts related to lipid metabolism and cell wall remodeling in order to cope with the host body temperature. Paracoccidioides spp. cells also induce transcripts/enzymes of the methylcitrate cycle (MCC), a pathway responsible for propionyl-CoA metabolism. Propionyl-CoA is a toxic compound formed during the degradation of odd-chain fatty acids, branched chain amino acids and cholesterol. Therefore, fungi require a functional MCC for full virulence and the ability to metabolize propionyl-CoA is related to the virulence traits in Paracoccidioides spp. On this way we sought to characterize the propionate metabolism in Paracoccidioides spp. The data collected showed that P. lutzii grows in propionate and activates the MCC by accumulating transcripts and proteins of methylcitrate synthase (MCS), methylcitrate dehydratase (MCD) and methylisocitrate lyase (MCL). Biochemical characterization of MCS showed that the enzyme is regulated by phosphorylation, an event not yet described. Proteomic analyses further indicate that P. lutzii yeast cells degrades lipids and amino acids to support the carbon requirement for propionate metabolism. The induction of a putative propionate kinase suggests that fungal cells use propionyl-phosphate as an intermediate in the production of toxic propionyl-CoA. Concluding, the metabolism of propionate in P. lutzii is under regulation at transcriptional and phosphorylation levels and that survival on this carbon source requires additional mechanisms other than activation of MCC.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Propionate metabolism in a human pathogenic fungus: proteomic and biochemical analyses

et al. 2020

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“…allowed to identify differently expressed genes during the mycelium-to-yeast transition and various host-interaction conditions, thus revealing the genus Paracoccidioides ' specific metabolic adaptations intimately related to its environment [27, 37–40]. Moreover, initial proteomic approaches performed in the members of the Paracoccidioides spp.…”

Section: Adaptationmentioning

confidence: 99%

ParacoccidioidesSpp.: Virulence Factors and Immune-Evasion Strategies

Camacho

Niño-Vega

2017

Mediators of Inflammation

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Paracoccidioides spp. are dimorphic fungal pathogens responsible for one of the most relevant systemic mycoses in Latin America, paracoccidioidomycosis (PCM). Their exact ecological niche remains unknown; however, they have been isolated from soil samples and armadillos (Dasypus novemcinctus), which have been proposed as animal reservoir for these fungi. Human infection occurs by inhalation of conidia or mycelia fragments and is mostly associated with immunocompetent hosts inhabiting and/or working in endemic rural areas. In this review focusing on the pathogen perspective, we will discuss some of the microbial attributes and molecular mechanisms that enable Paracoccidioides spp. to tolerate, adapt, and ultimately avoid the host immune response, establishing infection.

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“…The morphological transition of P. brasiliensis from mycelium to the yeast form is a key process for the infectivity of the fungus (Figure 2). There are several transcriptional profiling studies addressing which genes have increased or decreased mRNA accumulation during mycelium-to-yeast transitions (Felipe et al, 2005;Nunes et al, 2005;Bastos et al, 2007;Parente et al, 2008;Pereira et al, 2009), as well as during the conidia-to-yeast transition (Garcia et al, 2009). A P. brasiliensis biochip carrying sequences of 4,692 genes from this fungus was used to monitor gene expression at several time points of the myceliumto-yeast morphological shift (from 5 to 120 h) (Nunes et al, 2005).…”

Section: Identification Of Genes Which Have Their Mrna Accumulation Mmentioning

confidence: 99%

Molecular biology of the dimorphic fungi Paracoccidioides spp

Sturme

Puccia

Goldman

et al. 2011

Fungal Biology Reviews

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Paracoccidioides spp, herein commonly referred as P. brasiliensis, is the etiological agent of paracoccidioidomycosis (PCM), the most prevalent systemic mycosis endemic in Latin America. Many aspects of the biology of P. brasiliensis remain unknown, in particular its ecology and the apparent lack of a sexual reproduction stage in its life cycle. This review will highlight the current knowledge on the genetics and genomics of P. brasiliensis, its most important putative virulence factors and the challenges for developing genetic tools in this organism. P. brasiliensis is a dimorphic ascomycete fungus belonging to the order Onygenales, family Ajellomycetaceae. P. brasiliensis pathogenic yeast form is characterized by a multi-budding and-nucleate nature , with a high polymorphic cellular shape. Successful infection and dissemination by P. brasiliensis requires initial interaction of the fungus with host cells. The fungus has to adhere to host cells after which internalization of the fungus takes place. Gp43 is a 43-kDa glycoprotein that participates in the interaction with the host at different levels. There are very few putative virulence factors described in P. brasiliensis, among them an extracellular phospholipase B, a 32-kDa haloacid dehalogenase PbHAD32 that was shown to bind laminin, fibrinogen, and fibronectin, and to be important for initial adhesion to pulmonary epithelial cells, the pigment melanin, and the Rho-like GTPase Cdc42. The morphological transition of P. brasiliensis from mycelium to the yeast form is a key process for the infectivity of the fungus. There are several transcriptional profiling studies addressing which genes have increased or decreased mRNA accumulation during myceliumto-yeast transitions. Functional genomics studies in P. brasiliensis have been hampered by the absence of efficient molecular techniques that enable targeted gene inactivation in this fungus. However, an optimized Agrobacterium tumefaciens-mediated transformation method has been developed and was used to knock-down the Rho-like GTPase Cdc42 and the HAD-type hydrolase PbHAD32. A challenge for the future remains the development of mutagenesis methods that allow for the creation of targeted insertional gene mutants Paracoccidioides spp. The complete genome sequencing of three isolates of Paracoccidioides species provides the opportunity to perform more complete evaluations of the transcriptomic and proteomic data, and to understanding the biology and virulence of these important pathogenic fungi.

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Preferential transcription of Paracoccidioides brasiliensis genes: host niche and time-dependent expression

Abstract: Paracoccidioides brasiliensis causes infection through inhalation by the host of airborne propagules from the my

Cited by 8 publications

References 30 publications

Propionate metabolism in a human pathogenic fungus: proteomic and biochemical analyses

Propionate metabolism in a human pathogenic fungus: proteomic and biochemical analyses

ParacoccidioidesSpp.: Virulence Factors and Immune-Evasion Strategies

Molecular biology of the dimorphic fungi Paracoccidioides spp

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