Two dicot-infecting mastreviruses (family Geminiviridae) occur in Pakistan

Nahid, Nazia; Amin, Imran; Mansoor, Shahid; Rybicki, Edward P.; Walt, Eric van der; Briddon, R. W.

doi:10.1007/s00705-008-0133-7

Cited by 56 publications

(48 citation statements)

References 40 publications

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“…Sequence analysis of the full-length amino acid sequence of the putative Rep of SsHADV-1 showed that it shares the highest sequence identity with the Reps of chickpea chlorotic dwarf virus (25), bean yellow dwarf virus (26), and tobacco yellow dwarf virus (27) (Table S2). These three viruses that infect dicotyledonous plants belong to the genus Mastrevirus (Fig.…”

Section: Resultsmentioning

confidence: 99%

A geminivirus-related DNA mycovirus that confers hypovirulence to a plant pathogenic fungus

Fù

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

480

341

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Mycoviruses are viruses that infect fungi and have the potential to control fungal diseases of crops when associated with hypovirulence. Typically, mycoviruses have double-stranded (ds) or singlestranded (ss) RNA genomes. No mycoviruses with DNA genomes have previously been reported. Here, we describe a hypovirulenceassociated circular ssDNA mycovirus from the plant pathogenic fungus Sclerotinia sclerotiorum. The genome of this ssDNA virus, named Sclerotinia sclerotiorum hypovirulence-associated DNA virus 1 (SsHADV-1), is 2166 nt, coding for a replication initiation protein (Rep) and a coat protein (CP). Although phylogenetic analysis of Rep showed that SsHADV-1 is related to geminiviruses, it is notably distinct from geminiviruses both in genome organization and particle morphology. Polyethylene glycol-mediated transfection of fungal protoplasts was successful with either purified SsHADV-1 particles or viral DNA isolated directly from infected mycelium. The discovery of an ssDNA mycovirus enhances the potential of exploring fungal viruses as valuable tools for molecular manipulation of fungi and for plant disease control and expands our knowledge of global virus ecology and evolution.

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Section: Resultsmentioning

confidence: 99%

A geminivirus-related DNA mycovirus that confers hypovirulence to a plant pathogenic fungus

Fù

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

480

341

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“…The majority of mastreviruses infect monocot plants and are transmitted by leafhoppers (33)(34)(35). The genome of mastreviruses comprises four open reading frames (ORFs).…”

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confidence: 99%

Association of Satellites with a Mastrevirus in Natural Infection: Complexity of Wheat Dwarf India Virus Disease

Kumar

Singh

et al. 2014

J Virol

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In contrast to begomoviruses, mastreviruses have not previously been shown to interact with satellites. This study reports the first identification of the association of satellites with a mastrevirus in field-grown plants. Two alphasatellite species were detected in different field samples of wheat infected with Wheat dwarf India virus (WDIV), a Cotton leaf curl Multan alphasatellite (CLCuMA) and a Guar leaf curl alphasatellite (GLCuA). In addition to the alphasatellites, a betasatellite, Ageratum yellow leaf curl betasatellite (AYLCB), was also identified in the wheat samples. No begomovirus was detected in the wheat samples, thus establishing association of the above-named satellites with WDIV. Agrobacterium-mediated inoculation of WDIV in wheat, in the presence of either of the alphasatellites or the betasatellite, resulted in infections inducing more severe symptoms. WDIV efficiently maintained each of the alphasatellites and the betasatellite in wheat. The satellites enhanced the level of WDIV DNA in wheat. Inoculation of the satellites isolated from wheat with various begomoviruses into Nicotiana tabacum demonstrated that these remain capable of interacting with the viruses with which they were first identified. Virus-specific small RNAs accumulated in wheat upon infection with WDIV but were lower in abundance in plants coinfected with the satellites, suggesting that both the alphasatellites and the betasatellite suppress RNA silencing. These results suggest that the selective advantage for the maintenance of the alphasatellites and the betasatellite by WDIV in the field is in overcoming RNA silencing-mediated host defense. IMPORTANCE Wheat is the most widely cultivated cereal crop in the world. A number of viruses are important pathogens of wheat, including the viruses of the genus

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“…PCR products of~2.8 kb were cloned into the pTZ57R/ T vector (Fermentas). Subsequently, RCA using w29 DNA polymerase (Fermentas) was employed as described previously (Nahid et al, 2008). Concatameric RCA products were digested with restriction endonucleases to yield presumed monomeric virus (or virusassociated) components of either~2.8 or~1.4 kb.…”

Section: Methodsmentioning

confidence: 99%

Genetic diversity and phylogeography of begomoviruses infecting legumes in Pakistan

Ilyas

Qazi

Mansoor

et al. 2010

Journal of General Virology

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Grain legumes are an important source of dietary protein across southern Asia, but they suffer extensive losses due to several viruses that are members of the genus Begomovirus (family Geminiviridae), which are collectively known as legume yellow mosaic viruses (LYMVs). Despite their economic importance, little attention has been paid to LYMVs in Pakistan and only partial sequences of virus isolates originating from this country are available in the databases. Here, a survey of LYMVs occurring across Pakistan is described. Complete sequences of 44 components (23 DNA-A, 19 DNA-B and 2 betasatellites) were determined. The results show that only the mungbean yellow mosaic India virus (MYMIV) is of agricultural significance in Pakistan having been isolated from all cultivated grain legumes examined. Mungbean yellow mosaic virus, a significant crop pathogen in India, was only identified in a weed, which together with a novel species of LYMV we reported earlier, represents the first LYMV identified in non-cultivated plants. MYMIV was shown to occur as two types in Pakistan that show phylogeographical segregation. Additionally, two begomovirus species not considered pathogens of legumes and a betasatellite were isolated. This is of grave concern since it suggests that the presumed genetic isolation of the LYMVs in legumes may be being breached. LYMVs show little, if any, evidence of interspecific recombination with non-legume infecting begomoviruses. Thus, either recombination with non-legume viruses or interaction with betasatellites, which are host range and pathogenicity determining satellites of begomoviruses, could lead to the appearance of more aggressive virus variants/strains affecting legumes.

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Two dicot-infecting mastreviruses (family Geminiviridae) occur in Pakistan

Cited by 56 publications

References 40 publications

A geminivirus-related DNA mycovirus that confers hypovirulence to a plant pathogenic fungus

A geminivirus-related DNA mycovirus that confers hypovirulence to a plant pathogenic fungus

Association of Satellites with a Mastrevirus in Natural Infection: Complexity of Wheat Dwarf India Virus Disease

Genetic diversity and phylogeography of begomoviruses infecting legumes in Pakistan

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