A geminivirus-related DNA mycovirus that confers hypovirulence to a plant pathogenic fungus

Yu, Xiao Fang; Li, Bo; Fù, Yànpíng; Jiāng, Dàohóng; Ghabrial, Said A.; Li, Guoqing; Peng, You-Liang; Xiè, Jiǎtāo; Chéng, Jiāsēn; Huang, Junbin; Yi, Xianhong

doi:10.1073/pnas.0913535107

Cited by 469 publications

(358 citation statements)

References 42 publications

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“…The wide phylogenetic diversity of mycoviruses is reflected by their current classification into seven dsRNA families and five ssRNA families (www.ictvonline.org), and many novel taxa are awaiting description (e.g., Preisig et al, 2000;Ahn and Lee, 2001;Márquez et al, 2007;Hammond et al, 2008;Liu et al, 2009;Yu et al, 2010). Mycoviruses with RNA genomes propagate within the cytoplasm or mitochondria of their hosts and are transmitted laterally between fungal strains through anastomoses.…”

Section: Introductionmentioning

confidence: 99%

Viruses accumulate in aging infection centers of a fungal forest pathogen

Vainio¹,

Müller²,

Korhonen³

et al. 2014

The ISME Journal

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Fungal viruses (mycoviruses) with RNA genomes are believed to lack extracellular infective particles. These viruses are transmitted laterally among fungal strains through mycelial anastomoses or vertically via their infected spores, but little is known regarding their prevalence and patterns of dispersal under natural conditions. Here, we examined, in detail, the spatial and temporal changes in a mycovirus community and its host fungus Heterobasidion parviporum, the most devastating fungal pathogen of conifers in the Boreal forest region. During the 7-year sampling period, viruses accumulated in clonal host individuals as a result of indigenous viruses spreading within and between clones as well as novel strains arriving via airborne spores. Viral community changes produced pockets of heterogeneity within large H. parviporum clones. The appearance of novel viral infections in aging clones indicated that transient cell-to-cell contacts between Heterobasidion strains are likely to occur more frequently than what was inferred from genotypic analyses. Intraspecific variation was low among the three partitivirus species at the study site, whereas the unassigned viral species HetRV6 was highly polymorphic. The accumulation of point mutations during persistent infections resulted in viral diversification, that is, the presence of nearly identical viral sequence variants within single clones. Our results also suggest that co-infections by distantly related viral species are more stable than those between conspecific strains, and mutual exclusion may play a role in determining mycoviral communities.

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Section: Introductionmentioning

confidence: 99%

Viruses accumulate in aging infection centers of a fungal forest pathogen

Vainio¹,

Müller²,

Korhonen³

et al. 2014

The ISME Journal

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“…The host for the coral-associated SCSDVs presented here is yet to be determined, but may include the algal endosymbiont (Symbiodinium spp., because nanovirus/geminiviruses infect plants), the coral host itself (because circoviruses infect animals), or another member of the coral holobiont (Yu et al, 2010;Delwart and Li, 2012;Rosario et al, 2012;). However, a previous study examining viruses associated with Montastraea cavernosa coral colonies searched Symbiodinium EST libraries and found few similarities to SCSDVs suggesting that the SCSDVs associated with diseased corals in this study are infecting the coral animal.…”

Section: Discussionmentioning

confidence: 99%

Potential role of viruses in white plague coral disease

Soffer

Brandt

Correa³

et al. 2013

The ISME Journal

104

120

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White plague (WP)-like diseases of tropical corals are implicated in reef decline worldwide, although their etiological cause is generally unknown. Studies thus far have focused on bacterial or eukaryotic pathogens as the source of these diseases; no studies have examined the role of viruses. Using a combination of transmission electron microscopy (TEM) and 454 pyrosequencing, we compared 24 viral metagenomes generated from Montastraea annularis corals showing signs of WP-like disease and/or bleaching, control conspecific corals, and adjacent seawater. TEM was used for visual inspection of diseased coral tissue. No bacteria were visually identified within diseased coral tissues, but viral particles and sequence similarities to eukaryotic circular Rep-encoding single-stranded DNA viruses and their associated satellites (SCSDVs) were abundant in WP diseased tissues. In contrast, sequence similarities to SCSDVs were not found in any healthy coral tissues, suggesting SCSDVs might have a role in WP disease. Furthermore, Herpesviridae gene signatures dominated healthy tissues, corroborating reports that herpes-like viruses infect all corals. Nucleocytoplasmic large DNA virus (NCLDV) sequences, similar to those recently identified in cultures of Symbiodinium (the algal symbionts of corals), were most common in bleached corals. This finding further implicates that these NCLDV viruses may have a role in bleaching, as suggested in previous studies. This study determined that a specific group of viruses is associated with diseased Caribbean corals and highlights the potential for viral disease in regional coral reef decline.

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“…Chemical methods to control phytopathogenic fungi can affect human health and have environmental consequences. In an attempt to minimize dependence on chemical methods, alternative means of pathogen control have been investigated (Yu et al, 2010). One alternative is to use hypovirulent phytopathogenic fungal strains that can biologically control phytopathogenic fungi of the same or related species.…”

Section: Introductionmentioning

confidence: 99%

Detection of isometric, dsRNA-containing viral particles in Colletotrichum gloeosporioides isolated from cashew tree

Figueirêdo

Giancoli

et al. 2012

Trop. plant pathol.

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Fungi are disease-causing agents in plants and affect crops of economic importance. One control method is to induce resistance in the host by using biological control with hypovirulent phytopathogenic fungi. Here, we report the detection of a mycovirus in a strain of Colletotrichum gloeosporioides causing anthracnose of cashew tree. The strain C. gloeosporioides URM 4903 was isolated from a cashew tree (Anacardium occidentale) in Igarassu, PE, Brazil. After nucleic acid extraction and electrophoresis, the band corresponding to a possible double-stranded RNA (dsRNA) was purified by cellulose column chromatography. Nine extrachromosomal bands were obtained. Enzymatic digestion with DNAse I and Nuclease S1 had no effect on these bands, indicating their dsRNA nature. Transmission electron microscopic examination of extracts from this strain showed the presence of isometric particles (30-35 nm in diameter). These data strongly suggest the infection of this C. gloeosporioides strain by a dsRNA mycovirus. Once the hypovirulence of this strain is confirmed, the strain may be used for the biological control of cashew anthracnose. Key words: Anacardium occidentale, anthracnose, biological control, hypovirulence, mycovirus, phytopathogenic fungi.Fungi are the most important disease-causing agents in plants with serious economic impact. Chemical methods to control phytopathogenic fungi can affect human health and have environmental consequences. In an attempt to minimize dependence on chemical methods, alternative means of pathogen control have been investigated (Yu et al., 2010). One alternative is to use hypovirulent phytopathogenic fungal strains that can biologically control phytopathogenic fungi of the same or related species. Fungal hypovirulence is usually caused by mycoviral infection. Hypovirulent fungi cause milder infection and may induce hypovirulence in other pathogenic strains via viral transfer (Pearson et al., 2009).All currently known mycoviruses have isometric, double-stranded RNA (dsRNA)-containing particles, and are classified into three families: Chrysoviridae, Endornaviridae, Partitiviridae and Totiviridae. Particle size ranges from 25 to 50 nm in diameter, except for endornaviruses which do not produce virions (Chu et al., 2002;Ghabrial & Suzuki, 2009).In this communication, we report the presence of isometric dsRNA viral particles in a strain of Colletotrichum gloeosporioides (Penz.) Sacc., the causal agent of anthracnose in cashew tree.Colletotrichum gloeosporioides URM 4903 was isolated from anthracnose lesions on a cashew tree (Anacardium occidentale L.) in the town of Igarassu, Pernambuco, Brazil (7°50'3.74"S and 34°54'22.87"O), and deposited in the mycology collection (URM) at Federal University of Pernambuco, Recife, PE. This strain was chosen after preliminary tests revealed poor pathogenicity in detached cashew leaves and the presence of extrachromosomal bands in agarose gel electrophoresis (unpublished data).PDA (20% potato extract, 2% dextrose, 1.5% agar) and PD (20% potato extract,...

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A geminivirus-related DNA mycovirus that confers hypovirulence to a plant pathogenic fungus

Cited by 469 publications

References 42 publications

Viruses accumulate in aging infection centers of a fungal forest pathogen

Viruses accumulate in aging infection centers of a fungal forest pathogen

Potential role of viruses in white plague coral disease

Detection of isometric, dsRNA-containing viral particles in Colletotrichum gloeosporioides isolated from cashew tree

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