Two clusters of mutations map distinct receptor-binding sites of echovirus 11 for the decay-accelerating factor (CD55) and for canyon-binding receptors

Rezaykin, A.V.; Novoselov, A. V.; Sergeev, A.G.; Fadeyev, F. A.; Lebedev, S. V.

doi:10.1016/j.virusres.2009.06.004

Cited by 11 publications

(10 citation statements)

References 19 publications

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“…Rezaikin et al determined that a mutation at position 129 was related to altered cell tropism in E11. 56 A mutation in this position, P129Q, was also found in the E populations. Notably, this mutation also caused the substitution of a ClO 2 -reactive amino acid (proline) by a ClO 2 -stable one (glutamine).…”

Section: Resultsmentioning

confidence: 75%

“…Additionally, mutations S131N and M238V in the E populations are situated close to mutations at positions 132 and 240 identified by Stuart et al Rezaikin et al determined that a mutation at position 129 was related to cell tropism in E11. 56 A mutation in this position, P129Q, was also found in the E populations. Notably, this mutation also caused the substitution of a ClO 2 -reactive amino acid (proline) by a ClO 2 -stable one (glutamine).…”

Section: ■ Results and Discussionmentioning

confidence: 75%

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Resistance of Echovirus 11 to ClO₂ Is Associated with Enhanced Host Receptor Use, Altered Entry Routes, and High Fitness

Zhong

Carratalà

Shim

et al. 2017

Environ. Sci. Technol.

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Waterborne viruses can exhibit resistance to common water disinfectants, yet the mechanisms that allow them to tolerate disinfection are poorly understood. Here, we generated echovirus 11 (E11) with resistance to chlorine dioxide (ClO2) by experimental evolution, and we assessed the associated genotypic and phenotypic traits. ClO2 resistance emerged after E11 populations were repeatedly reduced (either by ClO2-exposure or by dilution) and then regrown in cell culture. The resistance was linked to an improved capacity of E11 to bind to its host cells, which was further attributed to two potential causes: first, the resistant E11 populations possessed mutations that caused amino acid substitutions from ClO2-labile to ClO2-stable residues in the viral proteins, which likely increased the chemical stability of the capsid toward ClO2. Second, resistant E11 mutants exhibited the capacity to utilize alternative cell receptors for host binding. Interestingly, the emergence of ClO2 resistance resulted in an enhanced replicative fitness compared to the less resistant starting population. Overall this study contributes to a better understanding of the mechanism underlying disinfection resistance in waterborne viruses, and processes that drive resistance development.

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Section: Resultsmentioning

confidence: 75%

Section: ■ Results and Discussionmentioning

confidence: 75%

Resistance of Echovirus 11 to ClO₂ Is Associated with Enhanced Host Receptor Use, Altered Entry Routes, and High Fitness

Zhong

Carratalà

Shim

et al. 2017

Environ. Sci. Technol.

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“…Receptors can efficiently target viruses for endocytosis or may be used to activate specific signaling pathways that facilitate entry. Decay-accelerating factor (DAF/CD55) has been implicated in many cases of multi-receptor tropism amongst the enteroviruses [1,2,3,4,5,6,7,8,9,10,11,12,13], and other microbial proteins [14] . Thus, extensive structural and biochemical studies of DAF interactions with various serotypes of Enteroviruses (EV) and Group B Coxsackieviruses (CVB) have presented mechanistic insights into how DAF functions as a co-receptor for enteroviruses [8,9,10,11,12].…”

Section: Introductionmentioning

confidence: 99%

Equilibrium and Kinetics of Sin Nombre Hantavirus Binding at DAF/CD55 Functionalized Bead Surfaces

Buranda

Swanson

Bondu

et al. 2014

Viruses

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Decay accelerating factor (DAF/CD55) is targeted by many pathogens for cell entry. It has been implicated as a co-receptor for hantaviruses. To examine the binding of hantaviruses to DAF, we describe the use of Protein G beads for binding human IgG Fc domain-functionalized DAF ((DAF)2-Fc). When mixed with Protein G beads the resulting DAF beads can be used as a generalizable platform for measuring kinetic and equilibrium binding constants of DAF binding targets. The hantavirus interaction has high affinity (24–30 nM; kon ~ 105 M−1s−1, koff ~ 0.0045 s−1). The bivalent (DAF)2-Fc/SNV data agree with hantavirus binding to DAF expressed on Tanoue B cells (Kd = 14.0 nM). Monovalent affinity interaction between SNV and recombinant DAF of 58.0 nM is determined from competition binding. This study serves a dual purpose of presenting a convenient and quantitative approach of measuring binding affinities between DAF and the many cognate viral and bacterial ligands and providing new data on the binding constant of DAF and Sin Nombre hantavirus. Knowledge of the equilibrium binding constant allows for the determination of the relative fractions of bound and free virus particles in cell entry assays. This is important for drug discovery assays for cell entry inhibitors.

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“…3 conserved because this structural protein is important for proper assembly of the viral capsid by the virus (Oberste et al 2004;Lukashev 2005). The P1 region is also important for receptor recognition, which is associated with the tropism of enterovirus (He et al 2002;Rezaikin et al 2009;Chen et al 2012).…”

Section: Discussionmentioning

confidence: 99%

Molecular Epidemiology of Echovirus 18 Circulating in Mainland China from 2015 to 2016

Chen

Guo

et al. 2019

Virol. Sin.

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Echovirus 18 (E18), a serotype of Enterovirus B (EV-B) species, is an important pathogen in aseptic meningitis. E18 had rarely been detected in mainland China, but became the predominant pathogen associated with viral encephalitis (VE) and meningitis in Hebei province for the first time in 2015. To investigate the molecular epidemiology and genetic characteristics of E18 in mainland China, sixteen E18 strains from patient throat swabs with hand, foot, and mouth disease (HFMD) in six provinces in China collected between 2015 and 2016, and four E18 strains isolated from 18 patient cerebrospinal fluid specimens with VE in Hebei Province in 2015 were obtained and sequenced. Combined with the sequences from the GenBank database, we performed an extensive genetic analysis. Phylogenetic analysis of VP1 gene sequences revealed that all E18 strains from mainland China after 2015 belonged to subgenotype C2. There were no obvious specific differences in phylogenetic and variation analyses of E18 genome sequences between HFMD and VE/meningitis strains. Potential multiple recombination may have occurred in the 5 0 -untranslated region and in the P2 and P3 nonstructural protein-encoding regions of E18 strains from China. The current E18 strains were potential multiplerecombinant viruses. Overall, these findings supported that E18 caused HFMD, VE, and meningitis, although there were no significant associations between clinical features and viral genomic characteristics.Keywords Genetic characteristics Á Echovirus 18 (E18) Á Genome Á Enterovirus Á Recombination Xiangpeng Chen and Tianjiao Ji have contributed equally to this work.

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Two clusters of mutations map distinct receptor-binding sites of echovirus 11 for the decay-accelerating factor (CD55) and for canyon-binding receptors

Cited by 11 publications

References 19 publications

Resistance of Echovirus 11 to ClO₂ Is Associated with Enhanced Host Receptor Use, Altered Entry Routes, and High Fitness

Resistance of Echovirus 11 to ClO₂ Is Associated with Enhanced Host Receptor Use, Altered Entry Routes, and High Fitness

Equilibrium and Kinetics of Sin Nombre Hantavirus Binding at DAF/CD55 Functionalized Bead Surfaces

Molecular Epidemiology of Echovirus 18 Circulating in Mainland China from 2015 to 2016

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Two clusters of mutations map distinct receptor-binding sites of echovirus 11 for the decay-accelerating factor (CD55) and for canyon-binding receptors

Cited by 11 publications

References 19 publications

Resistance of Echovirus 11 to ClO2 Is Associated with Enhanced Host Receptor Use, Altered Entry Routes, and High Fitness

Resistance of Echovirus 11 to ClO2 Is Associated with Enhanced Host Receptor Use, Altered Entry Routes, and High Fitness

Equilibrium and Kinetics of Sin Nombre Hantavirus Binding at DAF/CD55 Functionalized Bead Surfaces

Molecular Epidemiology of Echovirus 18 Circulating in Mainland China from 2015 to 2016

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Resistance of Echovirus 11 to ClO₂ Is Associated with Enhanced Host Receptor Use, Altered Entry Routes, and High Fitness

Resistance of Echovirus 11 to ClO₂ Is Associated with Enhanced Host Receptor Use, Altered Entry Routes, and High Fitness