Two Clade A Phosphatase 2Cs Expressed in Guard Cells Physically Interact With Abscisic Acid Signaling Components to Induce Stomatal Closure in Rice

Min, Myung Ki; Choi, Eun‐Hye; Kim, Jin-Ae; Yoon, In Sun; Han, Seungsu; Lee, Yeongmok; Lee, Sang-Ho; Kim, Beom-Gi

doi:10.1186/s12284-019-0297-7

Cited by 24 publications

(19 citation statements)

References 41 publications

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“…Next, we monitored how much each ABA receptor affects ABA signaling using the protoplast system in planta. Rice protoplasts were co-transformed with the reporter, an ABA responsive synthetic promoter fused to the firefly luciferase gene, and vector constructs to overexpress different monomeric ABA receptors [21,42]. Under control condition, OsPYL/RCAR1 and OsPYL/RCAR3 exhibited high activity as compared with all other ABA receptors.…”

Section: Comparative Transcriptome Analysis Between Plants Over-exprementioning

confidence: 99%

Ectopic Expression of OsPYL/RCAR7, an ABA Receptor Having Low Signaling Activity, Improves Drought Tolerance without Growth Defects in Rice

Bhatnagar

Kim

Han

et al. 2020

IJMS

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Overexpression of abscisic acid (ABA) receptors has been reported to enhance drought tolerance, but also to cause stunted growth and decreased crop yield. Here, we constructed transgenic rice for all monomeric ABA receptors and observed that only transgenic rice over-expressing OsPYL/RCAR7 showed similar phenotype with wild type, without total yield loss when grown under normal growth condition in a paddy field. Even though transgenic rice over-expressing OsPYL/RCAR7 showed neither an ABA-sensitivity nor an osmotic stress tolerance in plate assay, it showed drought tolerance. We investigated the ABA-dependent interaction with OsPP2CAs and ABA signaling induction by OsPYL/RCAR7. In yeast two hybrid assay, OsPYL/RCAR7 required critically higher ABA concentrations to interact with OsPP2CAs than other ABA receptors, and co-immunoprecipitation assay showed strong interaction under ABA treatment. When ABA-responsive signaling activity was monitored using a transient expression system in rice protoplasts, OsPYL/RCAR7 had the lowest ABA-responsive signaling activity as compared with other ABA receptors. OsPYL/RCAR7 also showed weak suppression of phosphatase activity as compared with other ABA receptors in vitro. Transcriptome analysis of transgenic rice over-expressing OsPYL/RCAR7 suggested that only a few genes were induced similar to control under without exogenous ABA, but a large number of genes was induced under ABA treatment compared with control. We conclude that OsPYL/RCAR7 is a novel functional ABA receptor that has low ABA signaling activity and exhibits high ABA dependence. These results lay the foundation for a new strategy to improve drought stress tolerance without compromising crop growth.

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Section: Comparative Transcriptome Analysis Between Plants Over-exprementioning

confidence: 99%

Ectopic Expression of OsPYL/RCAR7, an ABA Receptor Having Low Signaling Activity, Improves Drought Tolerance without Growth Defects in Rice

Bhatnagar

Kim

Han

et al. 2020

IJMS

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“…In particular, ABA plays a major role in regulating stomatal function in adaptation to various environmental stresses, such as drought, cold, air pollutants, etc. [24][25][26][27] Inadequate stomatal closure at high vapor pressure deficit may be mediated by abscisic acid. 30 In addition, other phytohormones also contribute to stomatal aperture regulation, such as auxin, cytokinin, ethylene and salicylic acid.…”

Section: Sensitivity Of G S To Vpd Variation and The Concentration Ofmentioning

confidence: 99%

“…Increased levels of abscisic acid (ABA) while a plant is under stress promote stomatal closure and/or inhibit stomata opening in order to avoid excessive water loss. [24][25][26][27][28][29] The response of g s to VPD is correlated to the level of ABA. 30 The other phytohormones, including indole-3-acetic acid (IAA) and Gibberellins (GAs), are known to antagonize the effects of ABA on stomatal behavior.…”

Section: Introductionmentioning

confidence: 99%

Seasonal change in response of stomatal conductance to vapor pressure deficit and three phytohormones in three tree species

Zhang

Li³

et al. 2019

Plant Signaling & Behavior

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Stomatal behavior under global climate change is a central topic of plant ecophysiological research. Vapor pressure deficit (VPD) and phytohormones can affect stomata of leaves which can affect gas exchange characteristics of plant. The role of VPD in regulating leaf gas exchange of three tree species was investigated in Jinan, China. Experiments were performed in June, August, and October. Levels of three phytohormones (GA 3 , IAA, ABA) in the leaves of the three trees were determined by highperformance liquid chromatography in three seasons. The responses of stomatal conductance (g s ) to an increasing VPD in the leaves of the three trees had peak curves under different seasons, which differed from the prevailing response pattern of g s to VPD in most literature. The peak curve could be fitted with a Log-Normal Model (R 2 = 0.838-0.995). The VPD/RH values of the corresponding maximum of g s (g s-max -VPD/RH) could be calculated by fitted models. The g s-max -RH could be affected by environmental conditions, because of positive correlation between g s-max -RH and the mean monthly temperature in 2010 (R 2 > 0.81). Two typical stomatal models (the Leuning model and the optimal stomatal behavior model) were used to estimate g s values, but they poorly predicted g s in the three trees. The concentration of ABA was positively correlated to sensitivity in response of stomatal conductance to VPD in the leaves of the tree species during the different seasons. ARTICLE HISTORY

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“…Ã p < 0.05; ÃÃ p < 0.01; ÃÃÃ p < 0.001. Full-size  DOI: 10.7717/peerj.9266/ fig-3 which is specifically expressed in guard cells, plays an important role in stomatal movement (Min et al, 2019). However, the regulation mechanism of SAPK10 remains unclear and requires further study.…”

Section: Discussionmentioning

confidence: 99%

Legacy effects of historical grazing alter leaf stomatal characteristics in progeny plants

Yin

et al. 2020

PeerJ

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Grazing, one of the primary utilization modes of grassland, is the main cause of grassland degradation. Historical overgrazing results in dwarf phenotype and decreased photosynthesis of perennial plants. However, it remains unknown what the mechanism underlying of this legacy effect is, and the role of stomata in the resulting decreased photosynthesis also remains unclear. To address these questions, differences in stomatal density, length and width on both adaxial and abaxial epidermis were compared between overgrazing and ungrazed Leymus chinensis offspring by using rhizome buds cultivated in a greenhouse, and the correlation between photosynthetic capacity and stomatal behavior was also investigated. Our results showed that historical grazing significantly impacted phenotype, photosynthesis and stomatal traits of L. chinensis. The offspring plants taken from overgrazed parents were dwarfed compared to those taken from ungrazed parents, and the photosynthesis and stomatal conductance of plants with a grazing history decreased by 28.6% and 21.3%, respectively. In addition, stomatal density and length on adaxial and abaxial leaf surfaces were significantly increased; however, stomatal width on abaxial leaf surfaces of overgrazed L. chinensis was significantly decreased compared with ungrazed individuals. Moreover, the expression patterns of eight genes related to stomatal regulation were tested: seven were down-regulated (2–18 times) and one was up-regulated (three times). Genes, involved in ABC transporter and receptor-like serine/threonine protein kinase were down-regulated. These results suggest that legacy effects of historical grazing affect the stomatal conductance by decreasing the stomatal width in progeny plants, which thus results in lower photosynthesis. Furthermore, changes of stomatal traits and function were regulated by the inhibition of ABC transporter and serine/threonine protein kinase. These findings are helpful for future exploration of the possible mechanisms underlying the response of grassland plants to long-term overgrazing.

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Two Clade A Phosphatase 2Cs Expressed in Guard Cells Physically Interact With Abscisic Acid Signaling Components to Induce Stomatal Closure in Rice

Cited by 24 publications

References 41 publications

Ectopic Expression of OsPYL/RCAR7, an ABA Receptor Having Low Signaling Activity, Improves Drought Tolerance without Growth Defects in Rice

Ectopic Expression of OsPYL/RCAR7, an ABA Receptor Having Low Signaling Activity, Improves Drought Tolerance without Growth Defects in Rice

Seasonal change in response of stomatal conductance to vapor pressure deficit and three phytohormones in three tree species

Legacy effects of historical grazing alter leaf stomatal characteristics in progeny plants

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