Ectopic Expression of OsPYL/RCAR7, an ABA Receptor Having Low Signaling Activity, Improves Drought Tolerance without Growth Defects in Rice

Bhatnagar, Nikita; Kim, Rigyeong; Han, Seungsu; Song, Jaeeun; Lee, Gang Seob; Lee, Sang-Ho; Min, Myung Ki; Kim, Beom-Gi

doi:10.3390/ijms21114163

Cited by 19 publications

(12 citation statements)

References 47 publications

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“…Ectopic expression of the RCAR family revealed that subfamily II members are particularly suitable for reducing transpiration without imposing a growth penalty in Arabidopsis ( Yang et al., 2016 ; Yang et al., 2019 ). Similar findings are reported for wheat ( Triticum aestivum ), poplar ( Populus x canescens ), and rice ( Oryza sativa ) by overexpressing TaPYL4, PcRCAR9, and OsPYL/RCAR7, which belong to subfamily II receptors ( Mega et al., 2019 ; Papacek et al., 2019 ; Bhatnagar et al., 2020 ). Alternatively, deficiency in PP2Cs contributes to a water-productive trait.…”

Section: Introductionsupporting

confidence: 79%

Abscisic acid agonists suitable for optimizing plant water use

Roeder

Liu²,

Doch³

et al. 2023

Front. Plant Sci.

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Climate change and overexploitation of groundwater resources cause constraints on water demand for agriculture, thus threatening crop productivity. For future food security, there is an urgent need for crops of high water use efficiency combined with high crop productivity, i.e. having high water productivity. High water productivity means efficient biomass accumulation at reduced transpiration. Recent studies show that plants are able to optimize carbon uptake per water transpired with little or no trade-off in yield. The phytohormone abscisic acid (ABA) plays a pivotal role in minimizing leaf transpiration and mediating enhanced water productivity. Hence, ABA and more chemically stable ABA agonists have the potential to improve crop water productivity. Synthesis, screening, and identification of suitable ABA agonists are major efforts currently undertaken. In this study, we used yeast expressing the plant ABA signal pathway to prescreen ABA-related cyano cyclopropyl compounds (CCPs). The yeast analysis allowed testing the ABA agonists for general toxicity, efficient uptake, and specificity in regulating different ABA receptor complexes. Subsequently, promising ABA-mimics were analyzed in vitro for ligand-receptor interaction complemented by physiological analyses. Several CCPs activated ABA signaling in yeast and plant cells. CCP1, CCP2, and CCP5 were by an order of magnitude more efficient than ABA in minimizing transpiration of Arabidopsis plants. In a progressive drought experiment, CCP2 mediated an increase in water use efficiency superior to ABA without trade-offs in biomass accumulation.

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Section: Introductionsupporting

confidence: 79%

Abscisic acid agonists suitable for optimizing plant water use

Roeder

Liu²,

Doch³

et al. 2023

Front. Plant Sci.

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“…To go further into the potential role of the region of cA ancestry in the adaptation of Asian rice to Africa, we analysed the gene content of this region. Out of a total of 46 genes underlying this introgression, OsABP, OsCNGC, OsPP2C55, OsPP2C56, OsPYL/RCAR7, OsPYL/RCAR8, OsPYL7, and Prol-17 have functions directly related to responses to abiotic stresses such as salt stress and water stress (Bhatnagar et al 2020 ; González-Guzmán et al 2014 ; Han et al 2019 ; Macovei et al 2012 ; Macovei and Tuteja 2012 ; Min et al 2020 ; Nawaz et al 2014 ; Rodriguez 1998 ; Singh et al 2014 ; Tian et al 2015 ; Xu et al 2015 ; Xue et al 2008 ; Yadav et al 2020 ; You et al 2014 ).…”

Section: Discussionmentioning

confidence: 99%

Traces of Introgression from cAus into Tropical Japonica Observed in African Upland Rice Varieties

Beye

Billot

Ronfort

et al. 2023

Rice

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Background Asian rice Oryza sativa, first domesticated in East Asia, has considerable success in African fields. When and where this introduction occurred is unclear. Rice varieties of Asian origin may have evolved locally during and after migration to Africa, resulting in unique adaptations, particularly in relation to upland cultivation as frequently practiced in Africa. Methods We investigated the genetic differentiation between Asian and African varieties using the 3000 Rice Genomes SNP dataset. African upland cultivars were first characterized using principal component analysis among 292 tropical Japonica accessions from Africa and Asia. The particularities of African accessions were then explored using two inference techniques, PCA-KDE for supervised classification and chromosome painting, and ELAI for individual allelic dosage monitoring. Key Results Ambiguities of local differentiation between Japonica and other groups pointed at genomic segments that potentially resulted from genetic exchange. Those specific to West African upland accessions were concentrated on chromosome 6 and featured several cAus introgression signals, including a large one between 17.9 and 21.7 Mb. We found iHS statistics in support of positive selection in this region and we provide a list of candidate genes enriched in GO terms that have regulatory functions involved in stress responses that could have facilitated adaptation to harsh upland growing conditions.

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“…Most of these pathways are functionally redundant and replaceable, so overexpression of the endogenous genes in any of these 2288 pathways is difficult to cause a significant improvement for tolerant phenotypes (Zhu, 2016). Their transformed lines remain at the testing stage (Hu et al, 2010;Xiang et al, 2017;Bhatnagar et al, 2020;Wang et al, 2020). Moreover, transformation may generate completely new interactions between the transgenes making them function differently from what is expected.…”

Section: Endogenous Proteins Are Regulated In Complex Networkmentioning

confidence: 99%

Three strategies of transgenic manipulation for crop improvement

Yang

et al. 2022

Front. Plant Sci.

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Heterologous expression of exogenous genes, overexpression of endogenous genes, and suppressed expression of undesirable genes are the three strategies of transgenic manipulation for crop improvement. Up to 2020, most (227) of the singular transgenic events (265) of crops approved for commercial release worldwide have been developed by the first strategy. Thirty-eight of them have been transformed by synthetic sequences transcribing antisense or double-stranded RNAs and three by mutated copies for suppressed expression of undesirable genes (the third strategy). By the first and the third strategies, hundreds of transgenic events and thousands of varieties with significant improvement of resistance to herbicides and pesticides, as well as nutritional quality, have been developed and approved for commercial release. Their application has significantly decreased the use of synthetic pesticides and the cost of crop production and increased the yield of crops and the benefits to farmers. However, almost all the events overexpressing endogenous genes remain at the testing stage, except one for fertility restoration and another for pyramiding herbicide tolerance. The novel functions conferred by the heterologously expressing exogenous genes under the control of constitutive promoters are usually absent in the recipient crops themselves or perform in different pathways. However, the endogenous proteins encoded by the overexpressing endogenous genes are regulated in complex networks with functionally redundant and replaceable pathways and are difficult to confer the desirable phenotypes significantly. It is concluded that heterologous expression of exogenous genes and suppressed expression by RNA interference and clustered regularly interspaced short palindromic repeats-cas (CRISPR/Cas) of undesirable genes are superior to the overexpression of endogenous genes for transgenic improvement of crops.

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Ectopic Expression of OsPYL/RCAR7, an ABA Receptor Having Low Signaling Activity, Improves Drought Tolerance without Growth Defects in Rice

Cited by 19 publications

References 47 publications

Abscisic acid agonists suitable for optimizing plant water use

Abscisic acid agonists suitable for optimizing plant water use

Traces of Introgression from cAus into Tropical Japonica Observed in African Upland Rice Varieties

Three strategies of transgenic manipulation for crop improvement

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