Two appendages homologous between basal bodies and centrioles are formed using distinct <i>Odf2</i> domains

Tateishi, Kazuhiro; Yamazaki, Y.; Nishida, Tomoki; Watanabe, Shin; Kunimoto, Koshi; Ishikawa, Hiroaki; Tsukita, Sachiko

doi:10.1083/jcb.201303071

Cited by 82 publications

(117 citation statements)

References 46 publications

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“…CPAP also cooperates with additional proteins, including CP110, in determining the length of nascent centrioles (Kohlmaier et al , 2009; Schmidt et al , 2009; Tang et al , 2009; Comartin et al , 2013; Lin et al , 2013; Sharma et al , 2016). Finally, centriole maturation is completed by the acquisition of subdistal and distal appendages (Paintrand et al , 1992; Tateishi et al , 2013). …”

Section: Introductionmentioning

confidence: 99%

Quantitative analysis of human centrosome architecture by targeted proteomics and fluorescence imaging

et al. 2016

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Centrioles are essential for the formation of centrosomes and cilia. While numerical and/or structural centrosomes aberrations are implicated in cancer, mutations in centriolar and centrosomal proteins are genetically linked to ciliopathies, microcephaly, and dwarfism. The evolutionarily conserved mechanisms underlying centrosome biogenesis are centered on a set of key proteins, including Plk4, Sas‐6, and STIL, whose exact levels are critical to ensure accurate reproduction of centrioles during cell cycle progression. However, neither the intracellular levels of centrosomal proteins nor their stoichiometry within centrosomes is presently known. Here, we have used two complementary approaches, targeted proteomics and EGFP‐tagging of centrosomal proteins at endogenous loci, to measure protein abundance in cultured human cells and purified centrosomes. Our results provide a first assessment of the absolute and relative amounts of major components of the human centrosome. Specifically, they predict that human centriolar cartwheels comprise up to 16 stacked hubs and 1 molecule of STIL for every dimer of Sas‐6. This type of quantitative information will help guide future studies of the molecular basis of centrosome assembly and function.

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Section: Introductionmentioning

confidence: 99%

Quantitative analysis of human centrosome architecture by targeted proteomics and fluorescence imaging

et al. 2016

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“…At the mother centriole, subdistal appendages stably anchor a subpopulation of microtubules which participate in centrosome positioning during interphase [104]. Subdistal appendages are structurally similar to the basal foot that decorates centrioles in sponge choanocytes and in multiciliated cells from a variety of animal species, and indeed their assembly relies on at least one common component [101,105,106]. Like subdistal appendages, the basal foot anchors microtubules required for controlling the position of the centrioles within the plane of the membrane, which in turn determines ciliary beating orientation [105,107,108].…”

Section: (Iv) Evolution Of Centrosome Structure In Animalsmentioning

confidence: 99%

Exploring the evolutionary history of centrosomes

Azimzadeh

2014

Phil. Trans. R. Soc. B

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The centrosome is the main organizer of the microtubule cytoskeleton in animals, higher fungi and several other eukaryotic lineages. Centrosomes are usually located at the centre of cell in tight association with the nuclear envelope and duplicate at each cell cycle. Despite a great structural diversity between the different types of centrosomes, they are functionally equivalent and share at least some of their molecular components. In this paper, we explore the evolutionary origin of the different centrosomes, in an attempt to understand whether they are derived from an ancestral centrosome or evolved independently from the motile apparatus of distinct flagellated ancestors. We then discuss the evolution of centrosome structure and function within the animal lineage.

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“…Cenexin is localized on these 2 types of appendages 35 and is involved in both ciliogenesis and the stabilization of centrosomal microtubules. 13 It remains to be determined if nucleolin could also have a function related to ciliogenesis.…”

Section: Discussionmentioning

confidence: 99%

“…The older one (called mother or mature centriole in contrast with the younger one called daughter or immature centriole) possesses 2 kinds of appendages. 11 The distal appendages are important during ciliogenesis to anchor the mother centriole to the plasma membrane 12,13 whereas the subdistal appendages are involved in microtubule anchoring at the centrosome. 13,14 Even though, proteomic analyzes have identified several proteins associated with the centrosomes, 15,16 their localization within the centrosome structure has only been characterized for a subset of proteins.…”

Section: Introductionmentioning

confidence: 99%

Centrosomal nucleolin is required for microtubule network organization

et al. 2015

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Nucleolin is a pleiotropic protein involved in a variety of cellular processes. Although multipolar spindle formation has been observed after nucleolin depletion, the roles of nucleolin in centrosome regulation and functions have not been addressed. Here we report using immunofluorescence and biochemically purified centrosomes that nucleolin colocalized only with one of the centrioles during interphase which was further identified as the mature centriole. Upon nucleolin depletion, cells exhibited an amplification of immature centriole markers surrounded by irregular pericentrin staining; these structures were exempt from maturation markers and unable to nucleate microtubules. Furthermore, the microtubule network was disorganized in these cells, exhibiting frequent non-centrosomal microtubules. At the mature centriole a reduced kinetics in the centrosomal microtubule nucleation phase was observed in live silenced cells, as well as a perturbation of microtubule anchoring. Immunoprecipitation experiments showed that nucleolin belongs to protein complexes containing 2 key centrosomal proteins, g-tubulin and ninein, involved in microtubule nucleation and anchoring steps. Altogether, our study uncovered a new role for nucleolin in restricting microtubule nucleation and anchoring at centrosomes in interphase cells.

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Two appendages homologous between basal bodies and centrioles are formed using distinct Odf2 domains

Abstract: Analysis of Odf2 deletion mutants reveals regions important for the formation of basal body transition fibers and centriole distal appendages and distinct regions required for basal feet and subdistal appendages.

Cited by 82 publications

References 46 publications

Quantitative analysis of human centrosome architecture by targeted proteomics and fluorescence imaging

Quantitative analysis of human centrosome architecture by targeted proteomics and fluorescence imaging

Exploring the evolutionary history of centrosomes

Centrosomal nucleolin is required for microtubule network organization

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