Two- and Three-Dimensional Confocal Microscopy of the Eye

Masters, Barry R.

doi:10.1007/978-1-4613-8366-6_18

Cited by 3 publications

(2 citation statements)

References 23 publications

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“…It is the enhanced axial resolution in a confocal microscope that permits the improved optical sectioning of thick specimens and their threedimensional reconstruction (54). In order to obtain the maximum axial resolution, and hence the best degree of optical sectioning, it is necessary to use a microscope objective with a large numerical aperture.…”

Section: Image Formationmentioning

confidence: 99%

Three-Dimensional Confocal Microscopy of the Living Human Eye

Masters

Böhnke

2002

Annu. Rev. Biomed. Eng.

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Three-dimensional confocal microscopy of the living eye is a major development in instrumentation for biomicroscopy of the eye. This noninvasive optical technology has its roots in the application of optics to reflected light imaging of the eye. These instrument developments began with Leeuwenhoek's use of his single lens microscope to investigate the structure of the eye. There followed a series of connected instruments: the ophthalmoscope, the slit lamp, the specular microscope, and the clinical confocal microscope. In vivo confocal microscopy produces high contrast, reflected light images or optical sections through the depth of living ocular tissue. Stacks of registered optical sections can be transformed by computer visualization techniques into three-dimensional volume images of ocular tissues: cornea, ocular lens, retina, and optic nerve. The clinical confocal microscope has resulted in new diagnostic techniques and new cellular descriptions of ocular disorders and pathology.

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Section: Image Formationmentioning

confidence: 99%

Three-Dimensional Confocal Microscopy of the Living Human Eye

Masters

Böhnke

2002

Annu. Rev. Biomed. Eng.

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“…This can be documented in computer animations or movies of rotating three-dimensional objects. Three-dimensional visualizations of the rabbit cornea, 14-16 rabbit lens, 17,18 and the human in vivo fundus and optic nerve 19 have been developed from confocal optical slices of ocular tissue. These three-dimensional visualizations permit the observer to interactively view the ocular tissue from any arbitrary viewpoint.…”

Section: Discussionmentioning

confidence: 99%

Optical tomography of the in vivo human lens: three-dimensional visualization of cataracts

Masters

1996

J. Biomed. Opt.

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An in vivo human lens containing a cataract has been visualized by a series of orthogonal slices made through a three-dimensional volume reconstruction. Data acquisition was made with a transformed series of 60 rotated Scheimpflug digital images. Each digital image represents the light scatter from the lens in a plane that contains the optic axis. At each angular position of the camera, a digital image of the in vivo ocular lens was acquired. The set of 60 Scheimpflug digital images was mathematically transformed into a new data set in which the images were oriented perpendicular to the optic axis of the eye. The transformed set of optical sections was aligned to correct for small eye movements during the data collection process. In order to visualize the volume of the in vivo human lens, slices were projected through the lens volumetric data set. The use of orthogonal slices to visualize lenticular light scatter represents a new technique to visualize human cataracts in vivo in three dimensions.

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