Twelve novel microsatellite markers for the Chinese golden loach Sinibotia superciliaris (Günther 1892)

Characterization of 27 novel microsatellite loci in Sinibotia superciliaris (Günther 1892) and cross-species transferability in Sinibotia reevesae (Chang 1944)

“…The cross‐species transferability of those markers was clearly high, which might be due to their close genetic relationships of the two species. Overall, the mean polymorphic information content (PIC) for S. superciliaris is 0.54, showing a relatively high level of polymorphism, which is consistent with previous reports (Liu et al., ; Xiong, Liu, Duan, & Chen, ). In summary, the novel polymorphic microsatellites isolated herein will not only facilitate further population genetic studies of the two species but also aid in distinguishing those two closely related species.…”

“…Protocols for high‐throughput Illumina paired‐end sequencing and data assembly of one single S. superciliaris individual were the same as previously reported by Liu et al. (). MIcroSAtellite (MISA) 1.0 (http://pgrc.ipk-gatersleben.de/misa/misa.html) was used to search for putative microsatellites.…”

“…To date, the studies for the two species are limited, most studies were restricted to basic biology (Li et al., ; Pu et al., ; Yang & Ding, ), karyotype analysis (Yue et al., ) and molecular phylogenetics (Bohlen et al., ; Šlechtová, Bohlen, Freyhof, & Ráb, ). Recently, one study reported the microsatellite marker isolation in S. superciliaris based on the Illumina paired‐end sequencing technology and the results indicated a relatively high level of genetic diversity for S. superciliaris (Liu, Wang, Xie, & Peng, ). Here, we developed 27 novel polymorphic tetra‐nucleotide microsatellite markers for S. superciliaris and further screened for cross‐species transferability in S. reevesae .…”

Genetic Diversity Evaluation of Two Loach Fishes and their Artificial Hybrid Population Based on 19 Polymorphic Microsatellite Loci

“…The mitochondrial genomes of these two loaches were also released, which provided valuable genetic markers for investigations on population genetics and conservation biology (Ye et al, 2013;Zou et al, 2017). Recently, two studies based on high-throughput sequencing developed microsatellite molecular markers in wild populations of S. superciliaris and S. reevesae, which provided important resources for further population and evolutionary studies (Liu et al, 2017;Wang et al, 2018).…”