Characterization of 27 novel microsatellite loci in Sinibotia superciliaris (Günther 1892) and cross-species transferability in Sinibotia reevesae (Chang 1944)

Abstract

The Chinese golden loach, Sinibotia superciliaris and its congener Sinibotia reevesae, are endemic to China and extraordinary similar in morphological traits. However, few genetic studies have been conducted on them, especially those based on nuclear markers. Here, we developed 27 novel polymorphic microsatellite markers fromS. superciliaris and further tested the cross-species transferability of those loci in S. reevesae. The cross-species amplification test showed that 19 loci demonstrate polymorphism in S. reevesae. The mean number of alleles (N A ) were both 3 in S. superciliaris and S. reevesae, the mean expected heterozygosities (H E ) were 0.57 and 0.54, the Shannon-Wiener Diversity Indices (SW) were 0.84 and 0.82, and the evenness (E) were 0.09 and 0.08, respectively. The polymorphic information content (PIC) showed a high level of polymorphism for the two species (mean 0.54 and 0.50, respectively). In addition, the cross-species transferability (100%) of those markers was clearly high, which confirmed that the microsatellite markers developed here could be used effectively for other related species.

Genetic Diversity Evaluation of Two Loach Fishes and their Artificial Hybrid Population Based on 19 Polymorphic Microsatellite Loci

“…In this study, a total of 19 microsatellite loci were successfully amplified and exhibited polymorphism in the four artificial breeding populations of S. reevesae, S. superciliaris, and their hybrid populations, implying that the microsatellite markers we developed in the previous research were effective (Wang et al, 2018). Among the 19 loci, only locus SS24 was deviated from HWE in Group A of S. superciliaris.…”

“…Previous research indicated the locus was high polymorphic when PIC > 0.50 (Botstein et al, 1980), the mean PIC in the four groups here was ranged from 0.685 to 0.818, suggesting high genetic diversity of the four groups in this study. The PIC in the wild populations of these two loaches also showed a high level of polymorphism, and the mean PIC in S. superciliaris and S reevesae was 0.54 and 0.5, respectively (Wang et al, 2018). Compared with the wild populations, the culture F1 populations of these two loaches and their hybrid progeny of F1 here had also maintained high genetic diversity, indicating the domestication process of these two loaches were considered to be in the early stage, and the same pattern also detected in the red-tail catfish (Zhou et al, 2021).…”

“…A total of 19 polymorphic microsatellite loci that can be amplified in both S. superciliaris, S. reevesae in our previous research (Wang et al, 2018) were chosen for genotyping and genetic assessment for this study (Table I). The 5′ ends of the forward primer of all the microsatellite loci were labelled with a FAM fluorochrome, and then used for PCR amplification and genotyping in four groups mentioned above.…”

“…The mitochondrial genomes of these two loaches were also released, which provided valuable genetic markers for investigations on population genetics and conservation biology (Ye et al, 2013;Zou et al, 2017). Recently, two studies based on high-throughput sequencing developed microsatellite molecular markers in wild populations of S. superciliaris and S. reevesae, which provided important resources for further population and evolutionary studies (Liu et al, 2017;Wang et al, 2018).…”