Tweedle cuticular protein BmCPT1 is involved in innate immunity by participating in recognition of Escherichia coli

Liang, Jinghong; Wang, Ting; Zhang, Xiang; He, Ningjia

doi:10.1016/j.ibmb.2014.11.004

Cited by 28 publications

(18 citation statements)

References 47 publications

(57 reference statements)

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“…Interestingly, a Twdl gene in B. mori , BmCPT1 , encodes a protein localized to the epicuticle, which also has roles in innate immunity. Overexpression of this gene induces antimicrobial peptide synthesis (Liang et al ., ), suggesting that Twdl proteins have additional roles in insects besides being structural components of the cuticle. These data highlight the developmental utilization of a transcription factor, Ftz‐f1, for distinct developmental events; in this case, moulting/cuticle renewal and immune response.…”

Section: Discussionmentioning

confidence: 99%

Hormonal control and target genes of ftz‐f1 expression in the honeybee Apis mellifera: a positive loop linking juvenile hormone, ftz‐f1, and vitellogenin

Mello

Aleixo

Pinheiro

et al. 2018

Insect Molecular Biology

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Ftz‐f1 is an orphan member of the nuclear hormone receptor superfamily. A 20‐hydroxyecdysone pulse allows ftz‐f1 gene expression, which then regulates the activity of downstream genes involved in major developmental progression events. In honeybees, the expression of genes like vitellogenin (vg), prophenoloxidase and juvenile hormone‐esterase during late pharate‐adult development is known to be hormonally controlled in both queens and workers by increasing juvenile hormone (JH) titres in the presence of declining levels of ecdysteroids. Since Ftz‐f1 is known for mediating intracellular JH signalling, we hypothesized that ftz‐f1 could mediate JH action during the pharate‐adult development of honeybees, thus controlling the expression of these genes. Here, we show that ftz‐f1 has caste‐specific transcription profiles during this developmental period, with a peak coinciding with the increase in JH titre, and that its expression is upregulated by JH and downregulated by ecdysteroids. RNAi‐mediated knock down of ftz‐f1 showed that the expression of genes essential for adult development (e.g. vg and cuticular genes) depends on ftz‐f1 expression. Finally, a double‐repressor hypothesis‐inspired vg gene knock‐down experiment suggests the existence of a positive molecular loop between JH, ftz‐f1 and vg.

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Section: Discussionmentioning

confidence: 99%

Hormonal control and target genes of ftz‐f1 expression in the honeybee Apis mellifera: a positive loop linking juvenile hormone, ftz‐f1, and vitellogenin

Mello

Aleixo

Pinheiro

et al. 2018

Insect Molecular Biology

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“…Insects are one of the most successful classes of organism, accounting for almost 60% of all described terrestrial species, as a result of their ability to adapt to a wide variety of habitats . Key to their success is the cuticle, which defines body shape and offers protection from environmental stresses such as insecticides, physical injury, dehydration and pathogenic microorganisms. The insect cuticle is primarily composed of cuticular proteins (CPs) and chitin.…”

Section: Introductionmentioning

confidence: 99%

Preliminary characterization of putative structural cuticular proteins in the malaria vector Anopheles sinensis

Zhou

Duan

Sun

et al. 2017

Pest Management Science

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“…The insertion sites of transgenic strains were investigated by using inverse PCR as described previously (Liang et al, 2014;Zhang et al, 2017c). Briefly, the genomic DNA from the G2 transgenic silkworm strains was extracted and treated with proteinase K as well as RNase.…”

Section: Antibody Preparationmentioning

confidence: 99%

“…After digesting with HaeIII, the samples were ligated using T4 DNA ligase for circularization. The PCR amplification was performed as described previously (Liang et al, 2014). All PCR products were cloned into PMD19-T vector (TakaRa) and sequenced at BGI (Beijing, China).…”

Section: Antibody Preparationmentioning

confidence: 99%

Transgenic characterization of two silkworm tissue‐specific promoters in the haemocyte plasmatocyte cells

Zhang

Weng

et al. 2017

Insect Molecular Biology

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Haemocytes play crucial roles in insect metabolism, metamorphosis, and innate immunity. As a model of lepidopteran insects, the silkworm is a useful model to study the functions of both haematopoiesis and haemocytes. Tissue-specific promoters are excellent tools for genetic manipulation and are widely used in fundamental biological research. Herein, two haemocyte-specific genes, Integrin β2 and Integrin β3, were confirmed. Promoter activities of Integrin β2 and Integrin β3 were evaluated by genetic manipulation. Quantitative real-time PCR and western blotting suggested that both promoters can drive enhanced green fluorescent protein (EGFP) specifically expressed in haemocytes. Further evidence clearly demonstrated that the transgenic silkworm exhibited a high level of EGFP signal in plasmatocytes, but not in other detected haemocyte types. Moreover, EGFP fluorescence signals were observed in the haematopoietic organ of both transgenic strains. Thus, two promoters that enable plasmatocytes to express genes of interest were confirmed in our study. It is expected that the results of this study will facilitate advances in our understanding of insect haematopoiesis and immunity in the silkworm, Bombyx mori.

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Tweedle cuticular protein BmCPT1 is involved in innate immunity by participating in recognition of Escherichia coli

Cited by 28 publications

References 47 publications

Hormonal control and target genes of ftz‐f1 expression in the honeybee Apis mellifera: a positive loop linking juvenile hormone, ftz‐f1, and vitellogenin

Hormonal control and target genes of ftz‐f1 expression in the honeybee Apis mellifera: a positive loop linking juvenile hormone, ftz‐f1, and vitellogenin

Preliminary characterization of putative structural cuticular proteins in the malaria vector Anopheles sinensis

Transgenic characterization of two silkworm tissue‐specific promoters in the haemocyte plasmatocyte cells

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