Preliminary characterization of putative structural cuticular proteins in the malaria vector <i>Anopheles sinensis</i>

Zhou, Dan; Duan, Baiyun; Sun, Yan; Ma, Lei; Zhu, Changliang; Shen, Bo

doi:10.1002/ps.4649

Cited by 22 publications

(15 citation statements)

References 51 publications

(104 reference statements)

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“…The expression of CPAP3-A1, CPAP3-C1, CPAP3-D1 and CPAP3-E2 genes were significantly induced in the oriental fruit fly (Bactrocera dorsalis) by malathion, the main pesticide used against this pest [31]. Interestingly, it has been documented that CP genes were over-expressed in deltamethrin-resistant strains of A. sinensis and Culex pipiens pallens, and pyrethroid-resistant strains of A. stephensi, A. gambiae and A. sinensis [38][39][40]. This indicated that the upregulation of CP genes is linked to insecticide resistance, while only a few of them were evidenced as involved in such a role.…”

Section: Discussionmentioning

confidence: 99%

Genomic and Transcriptomic Analysis Reveals Cuticular Protein Genes Responding to Different Insecticides in Fall Armyworm Spodoptera frugiperda

Zhu

Xiao

et al. 2021

Insects

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The fall armyworm (FAW), Spodoptera frugiperda, is a serious pest of crucial crops causing great threats to the food security of the world. It has evolved resistance to various insecticides, while the underlying molecular mechanisms remain largely unknown. Cuticular proteins (CPs), as primary components in cuticle, play an important role in insects’ protection against environmental stresses. Few of them have been documented as participating in insecticide resistance in several insect species. In order to explore whether CP genes of the FAW exhibit a functional role in responding to insecticides stress, a total of 206 CPs, classified into eight families, were identified from the genome of the FAW through a homology-based approach coupled with manual efforts. The temporal expression profiles of all identified CP genes across developmental stages and their responses to 23 different insecticides were analyzed using the RNA-seq data. Expression profiling indicated that most of the CP genes displayed stage-specific expression patterns. It was found that the expression of 51 CP genes significantly changed after 48 h exposure to 17 different insecticides. The expression of eight CP genes responding to four insecticides were confirmed by RT-PCR analysis. The results showed that their overall expression profiles were consistent with RNA-seq analysis. The findings provide a basis for further functional investigation of CPs implied in insecticide stress in FAW.

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Section: Discussionmentioning

confidence: 99%

Genomic and Transcriptomic Analysis Reveals Cuticular Protein Genes Responding to Different Insecticides in Fall Armyworm Spodoptera frugiperda

Zhu

Xiao

et al. 2021

Insects

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“…The insect cuticle is a complex exoskeleton which covers all the exposed tissues and protects insects against adverse environmental conditions, forms a physical barrier against the intrusion of pathogens and maintains their movement [1–4]. It is also the main route of insecticide penetration, and the intake of insecticide can be reduced by thickening or changing the chemical composition of the cuticle [5]. The cuticle is mainly composed of chitin and cuticular proteins (CPs) [6–9].…”

Section: Introductionmentioning

confidence: 99%

RNA interference-mediated knockdown of 3, 4-dihydroxyphenylacetaldehyde synthase affects larval development and adult survival in the mosquito Aedes aegypti

Jing

Zhang

et al. 2019

Parasites Vectors

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Background The cuticle is an indispensable structure that protects the mosquito against adverse environmental conditions and prevents pathogen entry. While most cuticles are hard and rigid, some parts of cuticle are soft and flexible to allow movement and blood-feeding. It has been reported that 3, 4-dihydroxyphenylacetaldehyde (DOPAL) synthase is associated with flexible cuticle formation in Aedes aegypti . However, the molecular function of DOPAL synthase in the ontogenesis of mosquito remains largely unknown. In this study, we characterized gene expression profiles of DOPAL synthase and investigated its functions in larvae and female adults of Aedes agypti by RNAi. Results Our results suggest that the expression of DOPAL synthase is different during development and the transcriptional level reached its peak at the female white pupal stage, and DOPAL synthase was more highly expressed in the cuticle and midgut than other tissues in the adult. The development process from larva to pupa was slowed down strikingly by feeding the first-instar larvae with chitosan/ DOPAL synthase dsRNA nanoparticles. A qRT-PCR analysis confirmed that the dsRNA-mediated transcription of the DOPAL synthase was reduced > 50% in fourth-instar larvae. Meanwhile, larval molt was abnormal during development. Transmission electron microscopy results indicated that the formation of endocuticle and exocuticle was blocked. In addition, we detected that the ds DOPAL synthase RNA caused significant mortality when injected into the female adult mosquitoes. Conclusions Our findings demonstrate that DOPAL synthase plays a critical role in mosquito larval development and adult survival and suggest that DOPAL synthase could be a good candidate gene in RNAi intervention strategies in mosquito control. Electronic supplementary material The online version of this article (10.1186/s13071-019-3568-7) contains supplementary material, which is available to authorized users.

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“…Insects possess a large number of cuticle proteins that contain chitin-binding domains and belong to different families [ 189 , 190 , 191 , 192 , 193 ]. Proteins with one or three chitin-binding domains (CBD) are members of the CPAP1 or CPAP3 family (Cuticle Proteins Analogous to Peritrophis) [ 194 , 195 , 196 , 197 , 198 ].…”

Section: The Molecular Toolbox For Natural Chitin Matrix Productionmentioning

confidence: 99%

Improving Polysaccharide-Based Chitin/Chitosan-Aerogel Materials by Learning from Genetics and Molecular Biology

Behr

Ganesan

2022

Materials

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Improved wound healing of burnt skin and skin lesions, as well as medical implants and replacement products, requires the support of synthetical matrices. Yet, producing synthetic biocompatible matrices that exhibit specialized flexibility, stability, and biodegradability is challenging. Synthetic chitin/chitosan matrices may provide the desired advantages for producing specialized grafts but must be modified to improve their properties. Synthetic chitin/chitosan hydrogel and aerogel techniques provide the advantages for improvement with a bioinspired view adapted from the natural molecular toolbox. To this end, animal genetics provide deep knowledge into which molecular key factors decisively influence the properties of natural chitin matrices. The genetically identified proteins and enzymes control chitin matrix assembly, architecture, and degradation. Combining synthetic chitin matrices with critical biological factors may point to the future direction with engineering materials of specific properties for biomedical applications such as burned skin or skin blistering and extensive lesions due to genetic diseases.

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Preliminary characterization of putative structural cuticular proteins in the malaria vector Anopheles sinensis

Abstract: These results establish an information framework for An. sinensis CPs that provides a basis for further molecular research. © 2017 Society of Chemical Industry.

Cited by 22 publications

References 51 publications

Genomic and Transcriptomic Analysis Reveals Cuticular Protein Genes Responding to Different Insecticides in Fall Armyworm Spodoptera frugiperda

Genomic and Transcriptomic Analysis Reveals Cuticular Protein Genes Responding to Different Insecticides in Fall Armyworm Spodoptera frugiperda

RNA interference-mediated knockdown of 3, 4-dihydroxyphenylacetaldehyde synthase affects larval development and adult survival in the mosquito Aedes aegypti

Improving Polysaccharide-Based Chitin/Chitosan-Aerogel Materials by Learning from Genetics and Molecular Biology

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