Tuwongella immobilis gen. nov., sp. nov., a novel non-motile bacterium within the phylum Planctomycetes

Seeger, Christian; Butler, Margaret K.; Yee, Benjamin; Mahajan, Mayank; Fuerst, John A.; Andersson, Siv G. E.

doi:10.1099/ijsem.0.002271

Cited by 26 publications

(12 citation statements)

References 25 publications

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“…The first report of the isolation of a planctomycete in axenic cultures is due to the work of James T. Staley (Staley, 1973). During the last decade, several members of Planctomycetes were isolated in pure culture and described as representing new genera, namely Schlesneria (Kulichevskaya et al, 2007b), Singulisphaera (Kulichevskaya et al, 2008;Kulichevskaya et al, 2012a), Zavarzinella (Kulichevskaya et al, 2009), Aquisphaera (Bondoso et al, 2011), Telmatocola (Kulichevskaya et al, 2012b), Paludisphaera (Kulichevskaya et al, 2016;Kaushik et al, 2020), Roseimaritima (Bondoso et al, 2015;Kumar et al, 2020b), Rubripirellula (Bondoso et al, 2015;Kallscheuer et al, 2019b), Fimbriiglobus (Kulichevskaya et al, 2017a), Tundrisphaera (Kulichevskaya et al, 2017b), Mariniblastus (Lage et al, 2017), Caulifigura (Kallscheuer et al, 2020a), Tuwongella (Seeger et al, 2017), Crateriforma (Peeters et al, 2020c), Fuerstia (Kohn et al, 2016;Kohn et al, 2019), Novipirellula (Kallscheuer et al, 2019c), Tautonia (Kovaleva et al, 2019;Jogler et al, 2020) Limnoglobus (Kulichevskaya et al, 2020b), Thalassoglobus (Kohn et al, 2020a;Rivas-Marin et al, 2020c), Symmachiella (Salbreiter et al, 2020), Gimesia (Scheuner et al, 2014;Kumar et al, 2020a;Wang et al, 2020), Stieleria (Sandargo et al, 2020;Storesund et al, 2020;Surup et al, 2020), Aureliella (Kallscheuer et al, 2020b), Maioricimonas (Rivas-Marin et al, 20...…”

Section: Cultured Planctomycetesmentioning

confidence: 99%

Planctomycetes as Host-Associated Bacteria: A Perspective That Holds Promise for Their Future Isolations, by Mimicking Their Native Environmental Niches in Clinical Microbiology Laboratories

Kaboré

Godreuil

Drancourt

2020

Front. Cell. Infect. Microbiol.

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Traditionally recognized as environmental bacteria, Planctomycetes have just been linked recently to human pathology as opportunistic pathogens, arousing a great interest for clinical microbiologists. However, the lack of appropriate culture media limits our future investigations as no Planctomycetes have ever been isolated from patients’ specimens despite several attempts. Several Planctomycetes have no cultivable members and are only recognized by 16S rRNA gene sequence detection and analysis. The cultured representatives are slow-growing fastidious bacteria and mostly difficult to culture on synthetic media. Accordingly, the provision of environmental and nutritional conditions like those existing in the natural habitat where yet uncultured/refractory bacteria can be detected might be an option for their potential isolation. Hence, we systematically reviewed the various natural habitats of Planctomycetes, to review their nutritional requirements, the physicochemical characteristics of their natural ecological niches, current methods of cultivation of the Planctomycetes and gaps, from a perspective of collecting data in order to optimize conditions and the protocols of cultivation of these fastidious bacteria. Planctomycetes are widespread in freshwater, seawater, and terrestrial environments, essentially associated to particles or organisms like macroalgae, marine sponges, and lichens, depending on the species and metabolizable polysaccharides by their sulfatases. Most Planctomycetes grow in nutrient-poor oligotrophic environments with pH ranging from 3.4 to 11, but a few strains can also grow in quite nutrient rich media like M600/M14. Also, a seasonality variation of abundance is observed, and bloom occurs in summer-early autumn, correlating with the strong growth of algae in the marine environments. Most Planctomycetes are mesophilic, but with a few Planctomycetes being thermophilic (50°C to 60°C). Commonly added nutrients are N-acetyl-glucosamine, yeast-extracts, peptone, and some oligo and macro-elements. A biphasic host-associated extract (macroalgae, sponge extract) conjugated with a diluted basal medium should provide favorable results for the success of isolation in pure culture.

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Section: Cultured Planctomycetesmentioning

confidence: 99%

Planctomycetes as Host-Associated Bacteria: A Perspective That Holds Promise for Their Future Isolations, by Mimicking Their Native Environmental Niches in Clinical Microbiology Laboratories

Kaboré

Godreuil

Drancourt

2020

Front. Cell. Infect. Microbiol.

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“…The major fatty acids (FA) detected in strain PL17 T were C 16:1 ω 5c and C 18:0 ( Table 1 ). Notably, the C 16:1 ω 5c FA, which is one of the most abundant FAs in strain PL17 T , Gemmata obscuriglobus UQM2246 T , Zavarzinella formosa A10 T , Telmatocola sphagniphila SP2 T , and Tuwongella immobilis MBLW1 T [21] , [24] , [42] , is absent in Fimbriiglobus ruber SP5 T and Limnoglobus roseus PX52 T [22] , [23] . The fatty acid βOH-C16 comprised 12.9% of total FAs in strain PL17 T and was also present in the acid hydrolysate of Limnoglobus roseus PX52 T (11.6%), Fimbriiglobus ruber SP5 T (8.2%) and Telmatocola sphagniphila SP2 T (4.9%).…”

Section: Resultsmentioning

confidence: 99%

“…Zavarzinella [21] , Telmatocola [24] , and Fimbriiglobus [22] were isolated from Sphagnum -dominated boreal wetlands. Finally, the genera Tuwongella [42] and Limnoglobus [23] were described based on characterization of strains obtained from freshwater lakes in Australia and Russia, respectively. The family Gemmataceae belongs to the order Gemmatales [13] and accommodates strictly aerobic, chemo-organotrophic planctomycetes with spherical or ellipsoidal cells, which occur singly, in pairs, or are assembled in large rosette-like clusters and dendriform-like structures [22] .…”

Section: Introductionmentioning

confidence: 99%

Frigoriglobus tundricola gen. nov., sp. nov., a psychrotolerant cellulolytic planctomycete of the family Gemmataceae from a littoral tundra wetland

Kulichevskaya

Ivanova

Naumoff

et al. 2020

Systematic and Applied Microbiology

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Planctomycetes of the family Gemmataceae are characterized by large genome sizes and cosmopolitan distribution in freshwater and terrestrial environments but their ecological functions remain poorly understood. In this study, we characterized a novel representative of this family, strain PL17 T , which was isolated from a littoral tundra wetland and was capable of growth on xylan and cellulose. Cells of this isolate were represented by pink-pigmented spheres that multiplied by budding and occurred singly or in short chains and aggregates. Strain PL17 T was obligately aerobic, mildly acidophilic chemoorganotrophic bacterium, which displayed good tolerance of low temperatures. The major fatty acids were C 18:0 , C 16:1 ω 5 , and βOH-C 16:1 ; the major polar lipid was trimethylornithine. The genome of strain PL17 T consisted of a 9.83 Mb chromosome and a 24.69 kb plasmid. The G + C contents of the chromosomal and plasmid DNA were 67.4 and 62.3 mol%, respectively. Over 8900 potential protein-coding genes were identified in the genome including a putative cellulase that contains a domain from the GH5 family of glycoside hydrolases. The genome of strain PL17 T contained one linked and one unlinked rRNA operons with 16S rRNA gene sequences displaying 94.5% similarity to that in Gemmata obscuriglobus UQM2246 T . Based on the results of comparative phenotypic, chemotaxonomic and phylogenomic analyses, we propose to classify strain PL17 T (= CECT 9407 T = VKM B-3467 T ) as representing a novel genus and species of the family Gemmataceae , Frigoriglobus tundricola gen. nov., sp. nov.

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“…The major fatty acids (FA) detected in PX52 T were C 18 : 1 ω7 c and C 18 : 0 ( Table 2 ). Notably, the FA C 18 : 1 ω7 c , which is by far the most abundant FA in PX52 T , was absent from Zavarzinella formosa A10 T , Telmatocola sphagniphila SP2 T [ 1 ], and Tuwongella immobilis MBLW1 T [ 6 ], and was present only in minor amounts in Fimbriiglobus ruber SP5 T (0.4%) and Gemmata obscuriglobus UQM2246 T (1.6%) [ 6 ]. The fatty acid βOH-C 16:0 comprised 11.6 % of total FAs in PX52 T and was also present in the acid hydrolysates of Fimbriiglobus ruber SP5 T (8.2%) and Telmatocola sphagniphila SP2 T (4.9%) ( Table 2 ).…”

Section: Full-textmentioning

confidence: 99%

“…The family Gemmataceae belongs to the order Planctomycetales and accommodates strictly aerobic, chemoorganotrophic planctomycetes with spherical or ellipsoidal cells, which occur singly, in pairs, or are assembled in large rosette-like clusters and dendriform-like structures [ 1 ]. This family currently includes five described genera, i.e., Gemmata [ 2, 3 ], Zavarzinella [ 4 ], Telmatocola [ 5 ], Fimbriiglobus [ 1 ] and Tuwongella [ 6 ]. Daughter cells of species of the genera Gemmata and Zavarzinella are highly motile, while members of the genera Telmatocola, Fimbriiglobus and Tuwongella possess non-motile swarm cells.…”

mentioning

confidence: 99%

Limnoglobus roseus gen. nov., sp. nov., a novel freshwater planctomycete with a giant genome from the family Gemmataceae

Kulichevskaya

Naumoff

Miroshnikov

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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The family Gemmataceae accommodates aerobic, chemoorganotrophic planctomycetes, which inhabit various freshwater ecosystems, wetlands and soils. Here, we describe a novel member of this family, strain PX52 T , which was isolated from a boreal eutrophic lake in Northern Russia. This isolate formed pink-pigmented colonies and was represented by spherical cells that occurred singly, in pairs or aggregates and multiplied by budding. Daughter cells were highly motile. PX52 T was an obligate aerobic chemoorganotroph, which utilized various sugars and some heteropolysaccharides. Growth occurred at pH 5.0–7.5 (optimum pH 6.5) and at temperatures between 10 and 30 °C (optimum 20–25 °C). The major fatty acids were C 18 : 1 ɷ7c, C 18 : 0 and βOH-C 16:0 ; the major intact polar lipid was trimethylornithine, and the quinone was MK-6. The complete genome of PX52 T was 9.38 Mb in size and contained nearly 8000 potential protein-coding genes. Among those were genes encoding a wide repertoire of carbohydrate-active enzymes (CAZymes) including 33 glycoside hydrolases (GH) and 87 glycosyltransferases (GT) affiliated with 17 and 12 CAZy families, respectively. DNA G+C content was 65.6 mol%. PX52 T displayed only 86.0–89.8 % 16S rRNA gene sequence similarity to taxonomically described Gemmataceae planctomycetes and differed from them by a number of phenotypic characteristics and by fatty acid composition. We, therefore, propose to classify it as representing a novel genus and species, Limnoglobus roseus gen. nov., sp. nov. The type strain is strain PX52 T (=KCTC 72397 T =VKM B-3275 T ).

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Tuwongella immobilis gen. nov., sp. nov., a novel non-motile bacterium within the phylum Planctomycetes

Cited by 26 publications

References 25 publications

Planctomycetes as Host-Associated Bacteria: A Perspective That Holds Promise for Their Future Isolations, by Mimicking Their Native Environmental Niches in Clinical Microbiology Laboratories

Planctomycetes as Host-Associated Bacteria: A Perspective That Holds Promise for Their Future Isolations, by Mimicking Their Native Environmental Niches in Clinical Microbiology Laboratories

Frigoriglobus tundricola gen. nov., sp. nov., a psychrotolerant cellulolytic planctomycete of the family Gemmataceae from a littoral tundra wetland

Limnoglobus roseus gen. nov., sp. nov., a novel freshwater planctomycete with a giant genome from the family Gemmataceae

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