Turnover rates of nitrogen stable isotopes in the salt marsh mummichog, Fundulus heteroclitus, following a laboratory diet switch

Logan, John M.; Haas, Heather L.; Deegan, Linda A.; Gaines, Emily

doi:10.1007/s00442-005-0277-z

Cited by 115 publications

(80 citation statements)

References 33 publications

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“…Such results indicate that metabolism is not always negligible in the isotope turnover of fish tissue (Herzka & Holt 2000, Marcogliese 2001, Harvey et al 2002. Similar results were also reported for whitefish Coregonus lavaretus (Perga & Gerdeaux 2005), juvenile mummichogs Fundulus heteroclitus (Logan et al 2006), and juvenile sand goby Pomatoschistus minutus (Guelinckx et al 2007).…”

Section: Discussionsupporting

confidence: 78%

Turnover and fractionation of nitrogen stable isotope in tissues of grass carp Ctenopharyngodon idellus

Xia¹,

Gao²,

Li³

et al. 2013

Aquacult. Environ. Interact.

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Determination of the isotopic turnover rate in the various tissues of an organism is one of the essential prerequisites for tracing the food sources of consumers and for elucidating trophic interactions in ecological studies using stable isotope analysis (SIA). Isotopic turnover and fractionation in the commercially important freshwater teleost grass carp Ctenopharyngodon idellus, however, are poorly understood so far. In the present study, we conducted a diet-switch experiment of C. idellus for 3 mo to assess nitrogen isotopic turnover and fractionation in different tissues of this species, including liver, muscle, and gill. The results revealed that turnover rates exhibited significant differences between tissues and increased in the sequence of gill < muscle < liver, owing to the differences in metabolic activities between the tissues. Contribution to longterm nitrogen isotopic turnover rates from metabolism was greatest for the liver (~70 to 77% metabolic contribution) and greatest from growth for the gill (86 to 90% contribution of net tissue increase). Nitrogen half-lives estimated with time-or growth-based models were, respectively, 29.9 d and 1.18-fold mass increase for liver, 68.3 d and 1.45-fold mass increase for muscle, and 115.4 d and 1.81-fold mass increase for gill. The nitrogen isotopic fractionation of all tissues was enriched by 3 to 4 ‰ relative to the nitrogen isotopic signature of the diet, with significant differences between the 3 types of tissues, probably owing to the different biochemical pathways and constituents between the various tissues. : 177-186, 2013 fractionation between trophic levels relative to carbon and sulfur (Post 2002). KEY WORDS: Ctenopharyngodon idellus · Nitrogen isotopic turnover · Metabolism · Growth · Fractionation Resale or republication not permitted without written consent of the publisher OPEN PEN ACCESS CCESSAquacult Environ Interact 3After a consumer is provided with an isotopically distinct diet, the isotopic ratios of its tissues will change as a consequence of 2 general processes: catabolic breakdown and anabolic replacement. The isotopic turnover rate depends on both the growth rate, representing the synthesis from the new diet in excess of breakdown; and the metabolic rate, representing the balanced rate of breakdown of old tissue synthesized during feeding on a previous diet and resynthesis of tissue components made from the new diet (Hesslein et al. 1993, MacAvoy et al. 2005. With known growth rates, the proportional contributions of metabolism and growth to stable isotopic turnover can be estimated from nonlinear regressions of the isotopic turnover trajectories based on both time and growth models (Buchheister & Latour 2010). Previous studies have showed that the contribution of metabolic replacement to the total isotopic turnover rate is negligible or of minor importance for the juveniles of ectothermic species, and growth is the primary factor driving the turnover of stable isotopes due to the low metabolic activities of ectothermic an...

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Section: Discussionsupporting

confidence: 78%

Turnover and fractionation of nitrogen stable isotope in tissues of grass carp Ctenopharyngodon idellus

Xia¹,

Gao²,

Li³

et al. 2013

Aquacult. Environ. Interact.

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“…This also raises some caution as to the applicability of these predictions to older life-stages, where individuals are likely to be slower growing and sexually mature (Hesslein et al, 1993;Herzka & Holt, 2000;Perga & Gerdeaux, 2005). Where experimental studies have been completed on larger or older fishes, and those with lower specific growth rates, the results suggest that metabolic replacement contributes a major proportion of total turnover, in some cases accounting for 80% of isotopic change in dorsal muscle (Suzuki et al, 2005;Logan et al, 2006;Tarboush et al, 2006). Heady and Moore (2013) revealed that catabolism contributed more to 15 N turnover in tissues with faster turnover rates, contributing 68% for fin compared to 0.7% for scales.…”

Section: Discussionmentioning

confidence: 99%

“…Rather than relying on data collected in the wild, an alternative approach is the use of experimental diet-switch studies completed in controlled conditions (Heady & Moore, 2013;Xia et al, 2013a, b;Busst & Britton, 2016). In these studies, diet tends to be fixed so that the food items have relatively consistent stable isotope values that should provide more reliable turnover estimates in the tissues (Logan et al, 2006). These approaches should also provide enhanced understandings of the mechanisms involved in isotopic replacement (Buchheister & Latour, 2010;Heady & Moore, 2013).…”

Section: Introductionmentioning

confidence: 99%

Tissue-specific turnover rates of the nitrogen stable isotope as functions of time and growth in a cyprinid fish

Busst

Britton

2017

Hydrobiologia

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Ecological applications of stable isotope data require knowledge on the isotopic turnover rate of tissues, usually described as the isotopic half-life in days (T 0.5 ) or the change in mass (G 0.5 ). Ecological studies increasingly analyse tissues collected nondestructively, such as fish fin and scales, but there is limited knowledge on their turnover rates. Determining turnover rates in situ is challenging, with ex situ approaches preferred. Correspondingly, T 0.5 and G 0.5 of the nitrogen stable isotope (d 15 N) were determined for juvenile barbel Barbus barbus (5.5 ± 0.6 g starting weight) using a diet-switch experiment. d 15 N data from muscle, fin and scales were taken during a 125 day post diet-switch period. Whilst isotopic equilibrium was not reached in the 125 days, the d 15 N values did approach those of the new diet. The fastest turnover rates were in more metabolically active tissues, from muscle (highest) to scales (lowest). Turnover rates were relatively slow; T 0.5 was 84 (muscle) to 145 (scale) days; G 0.5 was 1.39 9 body mass (muscle) to 2.0 9 body mass (scales), with this potentially relating to the slow growth of the experimental fish. These turnover estimates across the different tissues emphasise the importance of estimating half-lives for focal taxa at species and tissue levels for ecological studies.

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“…Compound specific isotope analyses (e.g. Popp et al 2007, Olson et al 2010) and/or the use of a rapid turnover tissue such as liver or blood that reflects short-term, local feeding (MacNeil et al 2006, Logan et al 2006) could help resolve this question, but this was not possible due to logistic and ethical constraints.…”

mentioning

confidence: 99%

Intrapopulation variations in diet and habitat use in a marine apex predator, the broadnose sevengill shark Notorynchus cepedianus

Abrantes¹,

Barnett²

2011

Mar. Ecol. Prog. Ser.

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Turnover rates of nitrogen stable isotopes in the salt marsh mummichog, Fundulus heteroclitus, following a laboratory diet switch

Cited by 115 publications

References 33 publications

Turnover and fractionation of nitrogen stable isotope in tissues of grass carp Ctenopharyngodon idellus

Turnover and fractionation of nitrogen stable isotope in tissues of grass carp Ctenopharyngodon idellus

Tissue-specific turnover rates of the nitrogen stable isotope as functions of time and growth in a cyprinid fish

Intrapopulation variations in diet and habitat use in a marine apex predator, the broadnose sevengill shark Notorynchus cepedianus

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