Turning up the Heat with Therapeutic Epigenome Editing

Zezulin, Alexandra U.; Musunuru, Kiran

doi:10.1016/j.stem.2017.12.013

Cited by 10 publications

(3 citation statements)

References 5 publications

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“…After the modification in Cas9 protein and the emergence of CRISPR/dCas9 technology, scientists start regulatory and reporter genes to investigate the ability of dCas9 for epigenome editing ( Zezulin and Musunuru, 2018 ). It was reported that the plant genome was modified by methylation and demethylation at target DNA and resultantly developed late-flowering phenotypes ( Adli, 2018 ).…”

Section: Combining Epigenetics and Gene Editing To Improve Stress Res...mentioning

confidence: 99%

Moving Beyond DNA Sequence to Improve Plant Stress Responses

Saeed

Chaudhry²,

Bakhsh³

et al. 2022

Front. Genet.

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Plants offer a habitat for a range of interactions to occur among different stress factors. Epigenetics has become the most promising functional genomics tool, with huge potential for improving plant adaptation to biotic and abiotic stresses. Advances in plant molecular biology have dramatically changed our understanding of the molecular mechanisms that control these interactions, and plant epigenetics has attracted great interest in this context. Accumulating literature substantiates the crucial role of epigenetics in the diversity of plant responses that can be harnessed to accelerate the progress of crop improvement. However, harnessing epigenetics to its full potential will require a thorough understanding of the epigenetic modifications and assessing the functional relevance of these variants. The modern technologies of profiling and engineering plants at genome-wide scale provide new horizons to elucidate how epigenetic modifications occur in plants in response to stress conditions. This review summarizes recent progress on understanding the epigenetic regulation of plant stress responses, methods to detect genome-wide epigenetic modifications, and disentangling their contributions to plant phenotypes from other sources of variations. Key epigenetic mechanisms underlying stress memory are highlighted. Linking plant response with the patterns of epigenetic variations would help devise breeding strategies for improving crop performance under stressed scenarios.

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Section: Combining Epigenetics and Gene Editing To Improve Stress Res...mentioning

confidence: 99%

Moving Beyond DNA Sequence to Improve Plant Stress Responses

Saeed

Chaudhry²,

Bakhsh³

et al. 2022

Front. Genet.

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“…Firstly, the endurance of gene activation or a repression effect mediated by CRISPR/Cas9 remains to be undetermined. It has been thought that epigenome-editing-induced gene activation or repression is short-term [ 90 , 91 ]. On the contrary, there was also evidence showing that a gene silencing effect mediated by the hit-and-run epigenome editing strategy could also be long-term and inheritable [ 31 ].…”

Section: Concluding Remarks and Future Perspectivesmentioning

confidence: 99%

Novel Epigenetic Techniques Provided by the CRISPR/Cas9 System

Xie

Zhou

Sun

et al. 2018

Stem Cells International

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Epigenetics classically refers to the inheritable changes of hereditary information without perturbing DNA sequences. Understanding mechanisms of how epigenetic factors contribute to inheritable phenotype changes and cell identity will pave the way for us to understand diverse biological processes. In recent years, the emergence of CRISPR/Cas9 technology has provided us with new routes to the epigenetic field. In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

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“…Third, environmentally induced epigenetic modification is transitory and can be reversed by further environmental changes, and is the basis for related recommendations about altering lifestyle and exposure to environmental factors in relation to methylation-based alterations in gene expression related to the development of cancer. Fourth, contemporary approaches to e-GE may only result in transient changes in target cells ( Zezulin and Musunuru 2018 ).…”

Section: Introductionmentioning

confidence: 99%