Turn-on fluorescent probe for dopamine detection in solutions and live cells based on in situ formation of aminosilane-functionalized carbon dots

Tang, Xiaonan; Liu, Yiming; Bai, Xiao-Bo; Yuan, Hao; Hu, Yi‐Kao; Xiao-ping, YU; Liao, Xun

doi:10.1016/j.aca.2021.338394

Cited by 32 publications

(7 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The carbon dots coated with alkoxysilane were prepared by a one-pot green method according to the reported literature. − d -(+)-Glucose (500 mg, 2.50 mmol) was dissolved in DI water, slowly dropped into the 3-aminopropyltriethoxysilane (1 mL, 4.27 mmol) solution, and then stirred at room temperature for 48 h. The color change of the solution, from colorless to dark brown, indicated that the carbon dots had been successfully synthesized. The dark brown solution was dialyzed (MWCO = 0.5–1 kDa) with Milli-Q water for 12 h and then lyophilized to obtain the final product.…”

Section: Methodsmentioning

confidence: 99%

Synthesis and Processing of Dynamic Covalently Crosslinked Polydextran/Carbon Dot Nanocomposite Hydrogels with Tailorable Microstructures and Properties

Yeh

2022

ACS Biomater. Sci. Eng.

View full text Add to dashboard Cite

Using functionalized nanoparticles to crosslink hydrophilic polymers is a growing theme of directly constructing nanocomposite (NC) hydrogels. Employing dynamic covalent chemistry at the nanoparticle–polymer interface is particularly attractive due to the spontaneous formation and reversible manner of dynamic covalent bonds. However, the structure and property modulation of the dynamic covalently crosslinked NC hydrogels has not been thoroughly discussed. Here, we fabricated NC hydrogels by using amine-functionalized carbon dots (CDs) to crosslink polydextran aldehyde (PDA) polymers through imine bond formation. The role of PDA with different oxidation degrees (i.e., PDA10, PDA30, and PDA50) in affecting the microstructures and properties of PDA@CD hydrogels was systematically investigated, showing that the PDA50@CD hydrogel presented the densest structure and the highest mechanical strength among the three PDA@CD hydrogels. The pH-responsiveness, 3D printing, electrospinning, and biocompatibility of PDA@CD hydrogels were also demonstrated, showing the great promise of using PDA@CD hydrogels for applications in biomedicine and biofabrication.

show abstract

Section: Methodsmentioning

confidence: 99%

Synthesis and Processing of Dynamic Covalently Crosslinked Polydextran/Carbon Dot Nanocomposite Hydrogels with Tailorable Microstructures and Properties

Yeh

2022

ACS Biomater. Sci. Eng.

View full text Add to dashboard Cite

show abstract

“…Fluorescent nano-carbon dots (nano-CDs) are carbon-rich zero-dimensional nanomaterials that have garnered significant attention in recent years due to their diverse range of environmentally friendly characteristics. − Nano-CDs are considered as promising alternatives to semiconductor quantum dots, organic dyes, fluorescent nanoclusters, and rare-earth emissive materials, owing to their metal-free nature, high water solubility, and low toxicity. − In addition to these significant properties, carbon dots (CDs) can be synthesized through various simple and cost-effective methods, exhibiting bright and tunable light emission, excellent photostability, and favorable biocompatibility. − Conversely, CDs have gained considerable attention in the fields of biology, biotechnology, and medicine since their introduction in 2004, owing to their remarkable outcomes in various biological systems. − These exceptional characteristics of nano-CDs make them highly desirable for multifunctional applications, including chem–bio sensing, − bioimaging, drug delivery, , cancer diagnosis, thermal therapy, dynamic therapy, etc. − While CDs hold great promise as functional fluorescent materials in the fields of nanoscience and technology, further improvement in their stability is necessary.…”

Section: Introductionmentioning

confidence: 99%

Fluorescent Carbon Dots with Enhanced Thermal Stability for Multicolor Bioimaging in Hot Conditions

Khan,

Qin,

Khan

et al. 2023

ACS Appl. Nano Mater.

View full text Add to dashboard Cite

Carbon dots (CDs) have recently garnered considerable attention as remarkable fluorescent nanomaterials. However, the production of thermally stable CDs still remains a significant challenge as it requires a stable structure resistant to thermal oxidation and effective control of aggregation sites. Herein, we have developed a strategy for producing fluorescent nano-CDs with a stable structure and high thermal resistance properties using citrazinic acid and urea via solid-state carbonization. These nano-CDs exhibit excellent performance at high temperatures ranging from 10 to 90 °C. The core structure of nano-CDs is functionalized with various C�O/C�N nitrogen and oxygen groups, which enhance the structural integrity of the fluorescent nano-CDs and protect them against thermal oxidation and the aggregation effect. The thermal stability of the nano-CDs was evaluated using state-ofthe-art techniques such as photoluminescent spectroscopy, UV−vis absorption spectroscopy, and decay lifetime measurements. Besides thermal resistance, nano-CDs exhibit green light emission with a maximum excitation of 400 nm and a peak quantum yield of 7.8%. These low-toxicity nano-CDs were successfully incubated with T-ca cancer cells, enabling stable multicolor imaging even at elevated temperatures (25−45 °C).

show abstract

“…Extensive research has been dedicated to developing fluorescent probes to effectively detect DA [ 19 ]. In addition, various probes such as graphene quantum dots, functionalized carbon quantum dots, metal organic framework materials, and nanoclusters have been developed for detecting DA [ [20] , [21] , [22] , [23] ]. Changes in light absorption, which are observed directly by the naked eye or quantified spectroscopically, are assessed in colorimetric detection [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

Smartphone-integrated colorimetric sensor array-based reader system and fluorometric detection of dopamine in male and female geriatric plasma by bluish-green fluorescent carbon quantum dots

Chellasamy

Ankireddy

Lee³

et al. 2021

Materials Today Bio

View full text Add to dashboard Cite

A simple, cost-effective system was developed for dopamine (DA) detection using green synthesized 1–6 nm honey-based carbon quantum dots (H-CQDs) exhibiting bluish green fluorescence. The H-CQDs exhibited emission at 445 nm, with a quantum yield of ∼44%. The H-CQDs were used as a probe for electron transfer based DA detection and changes in H-CQD color in the presence of DA. The H-CQDs were formed with polar functional groups and were highly soluble in aqueous media. In the fluorometric mode, the proposed system demonstrated high specificity toward DA and effective limit of detection (LOD) values of 6, 8.5, and 8 nM in deionized (DI) water, male geriatric plasma, and female geriatric plasma, respectively, in the linear range 100 nM–1000 μM. In the colorimetric mode, the color changed within 5 min, and the LOD was 163 μM. A colorimetric sensor array system was used to precisely detect DA with a smartphone-integrated platform using an in house built imaging application and an analyzer app. Additionally, no additives were required, and the H-CQDs were not functionalized. More importantly, the H-CQDs were morphologically and analytically characterized before and after DA detection. Because the sensor array-based system allows high specificity DA detection in both DI water and geriatric plasma, it will play an important role in biomedical applications.

show abstract

Turn-on fluorescent probe for dopamine detection in solutions and live cells based on in situ formation of aminosilane-functionalized carbon dots

Cited by 32 publications

References 40 publications

Synthesis and Processing of Dynamic Covalently Crosslinked Polydextran/Carbon Dot Nanocomposite Hydrogels with Tailorable Microstructures and Properties

Synthesis and Processing of Dynamic Covalently Crosslinked Polydextran/Carbon Dot Nanocomposite Hydrogels with Tailorable Microstructures and Properties

Fluorescent Carbon Dots with Enhanced Thermal Stability for Multicolor Bioimaging in Hot Conditions

Smartphone-integrated colorimetric sensor array-based reader system and fluorometric detection of dopamine in male and female geriatric plasma by bluish-green fluorescent carbon quantum dots

Contact Info

Product

Resources

About