Turbo-Mixing in Microplates

Mitre, Elisa; Schulze, Marcus; Cumme, Gerhard A.; Röβler, Fabian; Rausch, Torsten; Rhode, Heidrun

doi:10.1177/1087057106297565

Cited by 19 publications

(22 citation statements)

References 14 publications

(26 reference statements)

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“…Shakers can damage biomolecules due to large shear stress (Kunas and Papoutsakis 1990). Vortexers generate heat, foam, and thin films that may influence fluid composition (Mitre et al 2007). Magnetic elements can present an obstacle for the use of optical detection, and ultrasonic agitation can damage biomolecules or cells (Mitre et al 2007).…”

Section: Introductionmentioning

confidence: 99%

“…Vortexers generate heat, foam, and thin films that may influence fluid composition (Mitre et al 2007). Magnetic elements can present an obstacle for the use of optical detection, and ultrasonic agitation can damage biomolecules or cells (Mitre et al 2007). In addition, most mechanical methods are not effective on microplates of a higher microwell density (e.g., 384 or 1536 wells) due to the high surface tension of the liquids (Berg et al 2001).…”

Section: Introductionmentioning

confidence: 99%

“…A major barrier for rapid microplate analysis is the lower mixing efficiency due to smaller microwell volume (Walling et al 2007;Mitre et al 2007). In small microwells, viscous forces and diffusion dominate the microscale reagent mixing, while the inertia forces associated with bulk fluid convection become negligible.…”

Section: Introductionmentioning

confidence: 99%

“…Attempts to improve mixing in microplates have included shakers, vortexers, magnetic elements, and ultrasonic agitators (Mitre et al 2007). However, these approaches have not yet demonstrated efficient mixing.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Enhanced bioreaction efficiency of a microfluidic mixer toward high-throughput and low-cost bioassays

Lee

Yeo

et al. 2011

Microfluid Nanofluid

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Microscale bioreactors are an important tool in performing bioassays. The speed and efficiency of these devices is often limited by the rate of reagent mixing. In spite of the various micromixing approaches, the coupled mixing/reaction process has yet to be clearly understood. This article presents experimental and computational studies on the enhancement of bioreaction rates using a novel cilia reactor. In the experiments, a biotin-avidin assay and a DNA hybridization assay were conducted to show the benefit of a cilia bioreactor compared with a simple diffusion reactor. A cilia reactor showed a shorter reaction time for approaching equilibrium. A numerical computation examined the bioreaction rate of the cilia reactor compared with the diffusion for (1) a biotin-avidin assay, (2) an immunoassay, and (3) a DNA hybridization assay. The reaction rate was characterized for each assay using the Damköhler number (Da). When Da was greater than 10 2 , the ratio of reaction time for the diffusion to cilia reactors linearly increased with Da, which could also save reagent usage by lowering the concentration of reagent probes. However, when the system had a Da smaller than 10 2 , the reaction time of a cilia reactor could not be shortened because the assay was dominated by reaction rather than fluid mixing. The results offer a general approach for enhancing bioreaction rates by employing microfluidic mixers for a bioassay.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Enhanced bioreaction efficiency of a microfluidic mixer toward high-throughput and low-cost bioassays

Lee

Yeo

et al. 2011

Microfluid Nanofluid

View full text Add to dashboard Cite

show abstract

“…10 Bubble formation is an inevitable artifact when sample mixing is needed in the context of both standard and capillary well microplates. 11,12 The deleterious effect of bubbles on microplate measurement is well known, and the fact that they can accumulate at the top surface of the liquid in the form of foams exacerbates the problem when fluorescence measurements are required. Not surprisingly, assay preparation protocols typically advocate gentle handling 13 or centrifuging 14 to mitigate bubble presence.…”

Section: Icroplates Are a Standard Tool In Analyticalmentioning

confidence: 99%