New Enabling Technology

Hardy, Neil; Khoo, Ji Yi; Yang, Shoufeng; Eickhoff, Holger; Olechno, Joe; Ellson, Richard

doi:10.1002/9783527645251.ch16

Cited by 1 publication

(1 citation statement)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…3 In many cases, the frontier for the miniaturization of fluid phase assemblies has been pushed to volume regimes of just a few nanoliters. 4 One challenge is that miniaturization increases the ratio of the surface area to volume and accelerates both the evaporation of nanoscale solvents into room air and their contamination by chemicals in the atmosphere, such as water vapor, oxygen, nitrogencontaining compounds, and carbon dioxide. Techniques for managing air-sensitive and moisture-sensitive chemicals on a laboratory scale were established long ago, 5 and recent reviews of this topic are often somewhat informal.…”

Section: Introductionmentioning

confidence: 99%

Maintaining Microclimates during Nanoliter Chemical Dispensations Using Custom-Designed Source Plate Lids

et al. 2016

View full text Add to dashboard Cite

A method is described for using custom snap-on lids to protect chemicals in microtiter plates from evaporation and contamination. The lids contain apertures (diameter 1.5, 1.0, or 0.5 mm) through which the chemical building blocks can be transferred. The lid with 0.5 mm apertures was tested using a noncontact acoustic liquid handler; the 1.0 and 1.5 mm lids were tested using two tip-based liquid handlers. All of the lids reduced the rate at which solvents evaporated to room air, and greatly reduced the rate of contamination by water and oxygen from room air. In steady-state measurements, the lids reduced the rate of evaporation of methanol, 1-hexene, and water by 33% to 248%. In cycled experiments, the contamination of aqueous solvent with oxygen was reduced below detectability and the rate at which DMSO engorged atmospheric water was reduced by 81%. Our results demonstrate that the lids preserve the integrity of air-sensitive reagents during the time needed for different types of liquid handlers to perform dispensations. Controlling degradation and evaporation of chemical building blocks exposed to the atmosphere is increasingly useful as the reagent volume is reduced by advances in liquid handling technology, such as acoustic droplet ejection.

show abstract

Section: Introductionmentioning

confidence: 99%