Turbinaria ornata and its associated epiphytic Bacillus sp. A promising molecule supplier to discover new natural product approaches

Moubayed, Nadine; Houri, Hadeel Jawad Al; Bukhari, Sarah I.

doi:10.1016/j.sjbs.2021.12.041

Cited by 3 publications

(2 citation statements)

References 58 publications

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“…All selected presumptive isolated strains were further identified with PCR reaction following the genomic DNA 26 . DNA concentration and quality were determined using the genova nanodrop (Italy) and 1.5% agarose gel electrophoresis; positive samples showed bands ranging from 200-600bp for the different sets of primers used.…”

Section: Molecular Identificationmentioning

confidence: 99%

Focus On Prebiotic and Probiotic Preventive and Therapeutic Diet Approaches to Restore Gut Homeostasis in Rodent Models of Autism

R.M. Alsubaiei,

A. Alfawaz,

M. Hassan

et al. 2024

Int J Life Sci Pharm Res

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Although variable diet approaches shaped the gut bacterial abundance differently, they are considered the core factors in modulating gut microbiota throughdifferent mechanisms. Our aim in this study is to focus on the dietary candidates that could maintain a healthy gut microbiota by promoting commensal bacteria and reducingor inhibiting pathogens. In the current study, our objective is to demonstrate that a novel combined dietary approach of pre-and probiotic mixtures could be more effectivein inducing significant improvements in gut bacterial composition as a target to treat ASD. Luteolin complex veggie capsules (Swanson, USA), artichoke extract, as well asyogurt and Lactobacillus rhamnosus veggie capsules (5 billion CFU) (Swanson, USA), recently known as Lacticaseibacillus rhamnosus were selected as prebiotics and probioticsrespectively and were screened for their protective and therapeutic effects on the gut microbiota. They were all administered orally over 5 weeks to propionic acid PPAtreatedrats as rodent models of Autism (groups 4-10). Their results were compared to the control animal group undertaking a standard diet (group 1) and to post and pre-PPA-treated groups (groups 2 and 3). Lactobacillus and Bifidobacterium were noted as the most dominant, non-fluctuating strains in response to all diet intakes, and theynegatively influenced the presence of Proteobacteria (Enterobacteriaceae), which decreased in almost all treated groups during weeks 2 and 3, and 4.Interestingly, Clostridiumsp. was observed to be high in number at week 1, mainly in the control group (1) and pre and post-PPA groups (2 and 3), but were inhibited in all treated groups over theextended treatment period. Bacteroidetes were positively increased with the high presence of Lactobacillus and Bifidobacterium spp. Levels throughout the experiment in almostall animal groups. Thus, both prebiotics and probiotics study designed candidates (Luteolin complex capsules, artichoke extract, yogurt, and L. rhamnosus capsules) inhibitedClostridium sp., promoted commensal bacteria, specifically Lactobacillus and Bifidobacterium spp., restored the normal gut bacterial niches and therefore could be consideredas potential promising dietary approaches for maintaining healthy physical and microbial gut strategies.

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Section: Molecular Identificationmentioning

confidence: 99%

Focus On Prebiotic and Probiotic Preventive and Therapeutic Diet Approaches to Restore Gut Homeostasis in Rodent Models of Autism

R.M. Alsubaiei,

A. Alfawaz,

M. Hassan

et al. 2024

Int J Life Sci Pharm Res

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“…Besides, phenol can cause damage to cell wall structures as well. The previous study reported that marine bacillus commonly produces antimicrobial substances such as bacteriocins and other peptide groups (Moubayed et al, 2022).…”

Section: Antibacterial Assaymentioning

confidence: 99%

Antibacterial Activity and GC–MS Based Metabolite Profiles of Indonesian Marine Bacillus

Murniasih

P²,

Untari³

2022

Indonesian J Pharm

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Investigating Indonesian marine bacteria producing active compounds is key to finding a cultivable source of marine drugs. Screening the potential strain as well as profiling the active compounds are important steps to identifying the targeted substances. Methods used in this study were isolated some Bacillus strains from several marine environments in Indonesia, evaluated the antibacterial activity, and characterized the secondary metabolite using GC-MS spectroscopy. Several active antimicrobial compounds derived from marine microorganisms were identified using GC-MS such as pyrrolo [1,2-a] pyrazine-1,4-dione, octatriacontyl pentafluoropropionate. We found that some marine bacillus showed antimicrobial activity, such as B. flexus, B. tequilensis, B subtilis, and Bacillus sp. Profiling of metabolites on GC-MS showed the presence of several bioactive compounds in the ethyl acetate extract, which were identified to be nitrogen compounds such as pyrrolo[1,2-a]pyrazine-1,4-dione, phthalates compounds (butyl isohexyl ester and 1,2 benzendicarboxilate bis (2-etilhexyl) ester), and dibutyl phthalate. Some phenolic compounds also were found, such as tris (2,4-di-ter-butilfenil) fosfat, phenol, 2,4-bis (1,1-dimethyl ethyl), and phenol 3,5-bis (1,1-dimethyl ethyl). Finally, fatty acid derivatives such as n-hexadecanoic acid, cis-vaccenic acid, 7-hexadecene, farnesol isomer A, and stigmastan-3,5-diene were also identified in several marine bacillus.

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Evaluation of phyto-gallic acid as a potential inhibitor of Staphylococcus aureus efflux pump mediated tetracycline resistance: an in-vitro and in-silico study

Moubayed,

Alsabbagh,

Smiline

et al. 2024

Natural Product Research

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Plants contain many bioactive compounds with potent antibacterial and efflux pump inhibitory activity (EPI). In this study, gallic acid extracted from pomegranate molasses by analytical HPLC holds promise as an EPI drug for Staphylococcus aureus mediated tetracycline resistance, it lowered the bacterial resistance and reversed the mechanism via tet family efflux pump, using molecular technique and in-silico molecular docking analysis. Extracted gallic acid combined with tetracycline demonstrated a significant decrease in the minimal inhibitory concentration MIC compared to its single activity. Similarly, little growth and lower fluorescence of S. aureus were observed on ethidium bromide (2.5mg/mL) agar plates, indicating a reversible efflux pump mechanism and a potent EPI activity.Molecular docking demonstrated a promising affinity binding energy between gallic acid and tet efflux genes, opening a new baseline in bacterial infection treatment.PCR for tetK and Qac A/B genes failed to show any relation between tet genes and gallic acid.

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Turbinaria ornata and its associated epiphytic Bacillus sp. A promising molecule supplier to discover new natural product approaches

Cited by 3 publications

References 58 publications

Focus On Prebiotic and Probiotic Preventive and Therapeutic Diet Approaches to Restore Gut Homeostasis in Rodent Models of Autism

Focus On Prebiotic and Probiotic Preventive and Therapeutic Diet Approaches to Restore Gut Homeostasis in Rodent Models of Autism

Antibacterial Activity and GC–MS Based Metabolite Profiles of Indonesian Marine Bacillus

Evaluation of phyto-gallic acid as a potential inhibitor of Staphylococcus aureus efflux pump mediated tetracycline resistance: an in-vitro and in-silico study

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