“…They were applied to analysis from blood (Buzard et al, 1956), tissues (Herret and Buzard, 1960), milk (Cox and Heotis, 1963;Stone, 1964), and drugs (Cross et al, 1960). More recently some authors described different methods involving microbiological assays (Gang and Shaikh, 1972), gas-liquid chromatography (Ryan et al, 1975), column or thin-layer chromatographic separation followed by spectrophotometric assays (Wojtowicz and Diliberto, 1971; Zoni and Lauria, 1967;Bortoletti and Perlotto, 1968). However, the quantitative analysis of pharmaceutical preparations containing several nitrofurans, the physicochemical properties of which are very similar, has not yet been reported by these methods.…”