Turbidimetric Method: A New, Ecological, and Fast Way to Evaluate of Vancomycin Potency

Nascimento, Patrícia Aleixa do; Kogawa, Ana Carolina; Salgado, Hérida Regina Nunes

doi:10.1093/jaoacint/qsaa068

Cited by 9 publications

(2 citation statements)

References 28 publications

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“…One of the problems in monitoring and detecting occurrence of antibiotic resistant bacteria is cost and accessibility. The method employed is a composite of the standard turbimetric assay (Kavanagh, 1968;Nascimento et al, 2020) , in that the antibiotic was applied in broth, but quantified using spread plating method. It is a field expedient, rapid, high throughput, low-cost approach that has low equipment requirements.…”

Section: Discussionmentioning

confidence: 99%

Reduction of Antibiotic-Resistant Bacterial Counts in a Mangrove-Riverine System in Capiz, Philippines - Preliminary Data

Castillo

Añasco

Tan

et al. 2021

Israeli Journal of Aquaculture - Bamidgeh

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The anthropogenic impact of effluents and indiscriminate use of antibiotics in aquaculture promotes the emergence and proliferation of antibiotic resistant (ABR) bacteria. Comparative total bacterial and ABR counts were determined from 3 locations of the Cadimahan river of Capiz province, Philippines. The upstream station showed lower total bacterial but higher ABR counts, which is the inverse of the downstream station which showed higher total bacterial but lower ABR counts. The ABR counts accounted for 0.0173%, 0.0043% and 0.0002% of the total bacterial counts for upstream, midstream, and downstream, respectively, showing a strong inverse correlation of -0.84 in the Pearson correlation coefficient. This result suggests a remediating effect potentially mediated by microbial dynamics in the Mangrove-riverine system.

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Section: Discussionmentioning

confidence: 99%

Reduction of Antibiotic-Resistant Bacterial Counts in a Mangrove-Riverine System in Capiz, Philippines - Preliminary Data

Castillo

Añasco

Tan

et al. 2021

Israeli Journal of Aquaculture - Bamidgeh

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“…The results of the vancomycin final product content obtained using the proposed method were compared with the results obtained by a microbiological method using turbidimetry. 37…”

Section: Comparison Of Methodsmentioning

confidence: 99%

A New and Ecological Method to Quantify Vancomycin in Pharmaceutical Product by Infrared Spectrometry

Nascimento¹,

Kogawa

Salgado³

2021

ACSi

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Vancomycin, an antimicrobial, does not present quantitative method by infrared spectrometry in the literature for the evaluation of a pharmaceutical product. This technique is considered a clean alternative because in the main, there is no solvent involved and the generation of waste is reduced. So, the aim of this study was to develop and validate a new, ecological, low cost and fast method by infrared spectrometry using KBr and band between 1450–1375 cm–1. It was linear in the range of 1.0–2.0 mg/150 mg, with a correlation coefficient of 0.9994. Selective when the spectra of vancomycin reference and sample were compared. Precise by repeatability (2.29%) and intermediate precision (3.12%). Accurate with average recovery of 99.37% and robust when strength and compression time of the pellets and KBr brand were varied. Considering all the methods found in literature, there is not one using infrared spectrometry for quantitative purpose, so the method developed and validated could be considered an innovation and clean alternative. This is due to the fact that it is fast, easy to handle, low cost, and non-toxic as well as generating minimal waste. The method can be applied in the routine analysis of vancomycin dosage form and is an important option for the current and sustainable pharmaceutical analysis.

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Cephalothin: Review of Characteristics, Properties, and Status of Analytical Methods

Nascimento

Kogawa

Salgado

2021

Journal of AOAC INTERNATIONAL

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Background Cephalothin (CET), a first generation cephalosporin, is the most efficient cephalosporin against resistant microorganisms. Many studies found in literature and pharmacopeias proposes analytical methods and, as most commonly, HPLC and microbiological assays. Objective This paper shows a brief review of analytical method to quantify CET with a green analytical chemistry approach. Methods The research data were collected from the literature and official compendia. Results Most of the analytical methods to determine CET were performed by HPLC and agar diffusion in pharmaceuticals, blood, urine or water. Other analytical methods were found, as UV, Vis, iodometry, fluorimetry, IR/Raman, electrochemical among others, but, in less quantity. One important aspect is that these methods use organic and toxic solvents like methanol and acetonitrile, and only about 4% of the methods found uses water as solvent. Conclusions In this way, researches about analytical methods focused on green analytical chemistry for CET are of great importance and very relevant to optimize its analysis in pharmaceutical industries and to guarantee the quality of the product. More than just the development of new techniques it is possible to enhance of the ones that already exists applying the green analytical chemistry principles. In this way, it will be possible to reduce the environment impacts caused by these analytical procedures. Highlights This work shows a brief review of literature and pharmacopeias of analytical methods to quantify CET. Its quality control can be updated to meet the needs of current analytical chemistry and to fit into sustainable and eco-friendly analyzes.

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Turbidimetric Method: A New, Ecological, and Fast Way to Evaluate of Vancomycin Potency

Cited by 9 publications

References 28 publications

Reduction of Antibiotic-Resistant Bacterial Counts in a Mangrove-Riverine System in Capiz, Philippines - Preliminary Data

Reduction of Antibiotic-Resistant Bacterial Counts in a Mangrove-Riverine System in Capiz, Philippines - Preliminary Data

A New and Ecological Method to Quantify Vancomycin in Pharmaceutical Product by Infrared Spectrometry

Cephalothin: Review of Characteristics, Properties, and Status of Analytical Methods

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