Tuning the oxidase mimics activity of manganese oxides via control of their growth conditions for highly sensitive detection of glutathione

Zhang, Xiaodan; Mao, Xuanxiang; Li, Siqi; Dong, Wen‐Fei; Huang, Yuming

doi:10.1016/j.snb.2017.11.094

Cited by 67 publications

(20 citation statements)

References 66 publications

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“…The K m value is proven to be an indicator of affinity between enzyme and substrate. The smaller the K m value, the stronger the affinity between the substrate and the enzyme. − The value of K m in NiMnO 3 composites based on TMB as the substrate was much lower than that of Mn 3 O 4 flowers, CuMnO 2 nanoflakes, NiCo 2 O 4 , and rod-like MnOOH/Mn 3 O 4 , as shown in Table . This indicated that, compared with other nanoparticles, NiMnO 3 composites required a lower concentration of TMB to achieve the maximum reaction velocity.…”

Section: Resultsmentioning

confidence: 96%

Preparation of Flower-like NiMnO₃ as Oxidase Mimetics for Colorimetric Detection of Hydroquinone

Zheng

Liu

Lian

et al. 2021

ACS Sustainable Chem. Eng.

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Hierarchical flower-like NiMnO3 composites were facilely prepared by template-free hydrothermal treatment and calcined under mild conditions. Due to the strong ability to absorb oxygen, the as-prepared NiMnO3 composites were endowed with oxidase-like activity to catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to a blue product without H2O2. Investigations on the mechanism indicate that oxidase-like activity of NiMnO3 composites originates from superoxide radical generation. The oxidase-like activity of the NiMnO3 composites was selectively inhibited by hydroquinone (HQ), which could reduce oxTMB to colorless TMB and could result in significant blue color fading. With increasing HQ concentration, the absorbance at 652 nm decreased gradually. A colorimetric method based on the NiMnO3 composite was established for the detection of the concentration of HQ in water and practical samples. This assay was simple, rapid, sensitive, and selective for detecting HQ with a low detection limit of 0.68 μM and an excellent linear range of 1–85 μM. This colorimetric sensor could be effectively applied for the detection of HQ in real water samples with RSD < 3.89% (n = 3) and Er < 2.17% (n = 3). The above results illustrated that the NiMnO3 composites have outstanding potential for application in environmental chemistry.

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Section: Resultsmentioning

confidence: 96%

Preparation of Flower-like NiMnO₃ as Oxidase Mimetics for Colorimetric Detection of Hydroquinone

Zheng

Liu

Lian

et al. 2021

ACS Sustainable Chem. Eng.

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“…The limit of detection (LOD, calculated by 3σ/ S , where σ represents the standard deviation of blank signal and S represents the slope of the calibration curve) was 0.05 μM, which was comparable with or even better than other fluorescent or enzyme mimic-based methods (Table S1). ,− ,,− …”

Section: Resultsmentioning

confidence: 99%

“…In recent years, nanomaterial-based enzyme mimics (nanozymes) have been broadly investigated as new artificial enzymes owing to their remarkable properties including easy synthesis, good stability, low cost, and design flexibility, in comparison with those of natural enzymes. − So far, efforts have been made to explore nanozyme materials for GSH detection, such as Fe 3 O 4 nanoparticles, manganese oxides, graphene dots, Au nanoparticles, metal organic frameworks, and so on. Nevertheless, poor diffusion and low catalytic activity of those nanozymes restrict the applications in biological media.…”

mentioning

confidence: 99%

Discriminative Detection of Glutathione in Cell Lysates Based on Oxidase-Like Activity of Magnetic Nanoporous Graphene

et al. 2019

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As the most abundant intracellular biothiol, glutathione (GSH) plays a central role in many cellular functions and has been proved to be associated with numerous clinical diseases. Nevertheless, it is still a challenge to detect GSH over other mercaptoamino acids owing to their similar structures and activities. In this paper, magnetic nanoporous graphene (MNPG) nanocomposites were prepared for the first time through partial combustion of graphene oxide (GO) and ferric chloride. Due to the combination of porous graphene and magnetic nanoparticles, the MNPG nanocomposites exhibited large specific surface area, fast mass, and electron transport kinetics, resulting in remarkable oxidase mimic activity and easy separation. On the basis of the inhibition effect of GSH on the MNPG-catalyzed oxidation of thiamine, a novel and simple method for fluorescence determination of GSH was established. The sensor displayed a good linear response in the range of 0.2−20 μM toward GSH with a limit of detection of 0.05 μM. High sensitivity and selectivity facilitated its practical application for discriminative detection of GSH levels in PC12 cell lysates. The presented assay will be a simple and powerful tool to monitor intracellular GSH levels for biomedical diagnosis. Furthermore, the MNPG nanocomposites will provide insights to construct nanoporous graphene-based hybrids and push forward the advancement of porous graphene for wide applications.

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“…[ 25–27 ] In the past decades, a wide range of chemical fluorescent probes for detection of biothiols has been developed based on various mechanisms including cyclization with aldehyde, [ 28,29 ] Michael addition, [ 30–33 ] cleavage reactions by thiols, [ 34–36 ] and other reactions. [ 37–41 ] Michael addition is one of the most commonly used reactions for detection of thiol‐containing amino acids. Among the various Michael’s acceptors, maleimide moieties are in common use.…”

Section: Introductionmentioning

confidence: 99%

Characterization of a novel styrylbenzimidazolium‐based dye and its application in the detection of biothiols

Kolińska

Grzelakowska

2020

Luminescence

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A novel styrylbenzimidazolium dye containing a maleimide group 5 was synthesized and characterized using proton nuclear magnetic resonance spectroscopy and mass spectrometry. The photophysical properties [ultraviolet–visible (UV–vis) light absorption and fluorescence spectra, fluorescence quantum yield, and fluorescence lifetime] were investigated. Spectroscopic characterization of the novel styrylbenzimidazolium‐based dye under various conditions is presented and its usefulness to detect biothiols proved. The addition of biothiols [l‐cysteine (l‐Cys), l‐homocysteine (l‐Hcy), l‐glutathione (l‐GSH)] to compound 5 in phosphate buffer (0.1 M, pH 7.4) containing 10% CH3CN induced a 15–28‐fold enhancement in fluorescence intensity at 410 nm. The limits of detection of compound 5 for l‐Cys, l‐Hcy, and l‐GSH were estimated as 0.114, 0.118, and 0.059 μM, respectively. Evaluation of the cytotoxicity of 5 using the PrestoBlue assay for HeLa cells was also determined. The examined compound revealed a slight cytotoxicity against HeLa cells under experimental conditions.

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Tuning the oxidase mimics activity of manganese oxides via control of their growth conditions for highly sensitive detection of glutathione

Cited by 67 publications

References 66 publications

Preparation of Flower-like NiMnO₃ as Oxidase Mimetics for Colorimetric Detection of Hydroquinone

Preparation of Flower-like NiMnO₃ as Oxidase Mimetics for Colorimetric Detection of Hydroquinone

Discriminative Detection of Glutathione in Cell Lysates Based on Oxidase-Like Activity of Magnetic Nanoporous Graphene

Characterization of a novel styrylbenzimidazolium‐based dye and its application in the detection of biothiols

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Tuning the oxidase mimics activity of manganese oxides via control of their growth conditions for highly sensitive detection of glutathione

Cited by 67 publications

References 66 publications

Preparation of Flower-like NiMnO3 as Oxidase Mimetics for Colorimetric Detection of Hydroquinone

Preparation of Flower-like NiMnO3 as Oxidase Mimetics for Colorimetric Detection of Hydroquinone

Discriminative Detection of Glutathione in Cell Lysates Based on Oxidase-Like Activity of Magnetic Nanoporous Graphene

Characterization of a novel styrylbenzimidazolium‐based dye and its application in the detection of biothiols

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Preparation of Flower-like NiMnO₃ as Oxidase Mimetics for Colorimetric Detection of Hydroquinone

Preparation of Flower-like NiMnO₃ as Oxidase Mimetics for Colorimetric Detection of Hydroquinone