Discriminative Detection of Glutathione in Cell Lysates Based on Oxidase-Like Activity of Magnetic Nanoporous Graphene

Abstract

As the most abundant intracellular biothiol, glutathione (GSH) plays a central role in many cellular functions and has been proved to be associated with numerous clinical diseases. Nevertheless, it is still a challenge to detect GSH over other mercaptoamino acids owing to their similar structures and activities. In this paper, magnetic nanoporous graphene (MNPG) nanocomposites were prepared for the first time through partial combustion of graphene oxide (GO) and ferric chloride. Due to the combination of porous graphene and magnetic nanoparticles, the MNPG nanocomposites exhibited large specific surface area, fast mass, and electron transport kinetics, resulting in remarkable oxidase mimic activity and easy separation. On the basis of the inhibition effect of GSH on the MNPG-catalyzed oxidation of thiamine, a novel and simple method for fluorescence determination of GSH was established. The sensor displayed a good linear response in the range of 0.2−20 μM toward GSH with a limit of detection of 0.05 μM. High sensitivity and selectivity facilitated its practical application for discriminative detection of GSH levels in PC12 cell lysates. The presented assay will be a simple and powerful tool to monitor intracellular GSH levels for biomedical diagnosis. Furthermore, the MNPG nanocomposites will provide insights to construct nanoporous graphene-based hybrids and push forward the advancement of porous graphene for wide applications.

Oxidase‐Like Fe‐N‐C Single‐Atom Nanozymes for the Detection of Acetylcholinesterase Activity

“…Though various works have devoted to the study of peroxidase‐like activity of Fe‐based nanomaterials,1a,14 few Fe‐based nanomaterials with oxidase‐like activity were reported . To study the oxidase‐like nanozyme property of Fe‐N‐C SAzymes, 3,3′,5,5′‐tetramethylbenzidine (TMB) was employed as the allochroic substrates ( Figure A).…”

Highly Efficient Separation of Methylated Peptides Utilizing Selective Complexation between Lysine and 18-Crown-6

“…On the one hand, this study displays a preliminary result to illustrate the possibility of this protocol in the lysine methylated peptide separation and enrichment; however, more careful studies should be conducted to verify whether this protocol is efficient in real PTM enrichment from complex cell and tissue samples, which is undergoing in our laboratory. On the other hand, different from conventional strategies that focus on the designs of chromatographic stationary materials, , 18-crown-6 as an additive in mobile phase endows separation with a powerful modulation parameter, providing a novel idea to solve the problem in separation science. , …”

“…On the other hand, different from conventional strategies that focus on the designs of chromatographic stationary materials, 36,37 18-crown-6 as an additive in mobile phase endows separation with a powerful modulation parameter, providing a novel idea to solve the problem in separation science. 38,39 ■ ASSOCIATED CONTENT * sı Supporting Information…”

Graphene Oxide-Supported Lanthanide Metal–Organic Frameworks with Boosted Stabilities and Detection Sensitivities

“…First, 50 mg of PGO was mixed into 80 mL of concentrated HNO 3 and refluxed for 11 h under 85 °C and rigorous stirring. 28 Then the carboxylated PGO was filtered and washed with deionized water three times. After being dried in vacuum oven, the carboxylated PGO (PGO-COOH) was obtained.…”

“…PGO was prepared through a combustion reaction. , In brief, 1 g of GO was mixed with 1.0 mL of FeCl 3 solution under continuous sonication for 1 h. Then the mixture was filtered through filter paper so that the GO loaded with certain amounts of FeCl 3 was trapped on the filter paper. After the filter paper covered by GO was dried in a vacuum oven at 80 °C, the filter paper was treated at 450 °C for 2 min (timed with a stopwatch) in a muffle furnace preset at 450 °C.…”