Tuning the nanostructural properties of silver nanoparticles for optimised surface enhanced Raman scattering sensing of SARS CoV-2 spike protein

Abstract

In this work, development of fast, selective and highly sensitive sensor for detecting severe acute respiratory syndrome coronavirus 2 (known as SARS CoV-2 or COVID19) spike protein has been reported. Surface enhanced Raman spectroscopy (SERS) based direct detection of spike protein was investigated by fabricating silver nanoparticles decorated Si (AgNPs/Si) using a facile wet chemical process. Fabrication parameters such as immersion time and precursor concentration were varied; their corresponding morphological characteristics were well elucidated while assuring homogeneous decoration of AgNPs on Si substrate. Classical dye molecule such as rhodamine 6G (R6G) was utilised for the optimisation of fabricated sensor. Based on acquired intensity of R6G, the best sensor was selected, which was further employed for label free direction of spike protein. The developed sensor exhibited high sensitivity in pico-molar range with excellent reproducibility. Selectivity studies were demonstrated using DNA and H1N1 protein as well. The current findings will launch new state of art in the field of medical diagnostics.

Silicon/Silver nanocomposite-based surface-enhanced Raman spectroscopy (SERS) biosensor for ultrasensitive direct detection of deafness mutations in real system

“…SERS sensor (SSN) was fabricated with respect to our earlier reports by facile wet chemical strategy [6]. The aqueous DNA stock solutions (DMF 16 and DMF 34) stored in -18°C was taken and thawed.…”

“…Silver nanoparticles tend to attract and to create an engaging area with great electrical field that can enhance the signal read out of analyte molecules at their proximity. Label free detection of DNA as well mutations are possibly achieved with advanced SERS technology [5,6].…”

Electrochemical and optical-based systems for SARS-COV-2 and various pathogens assessment

“…Over the years, there has been significant progress in the advancement of electrochemical sensors relying on nanomaterials and polymers. These sensors have demonstrated enhanced sensitivity, selectivity, and detection limits for a diverse range of analytes [18,[53][54][55]. The establishment of contemporary electrochemical measurement was initiated through the advancement of diverse electrode materials and methodologies, such as carbon paste, gold, platinum, and glassy carbon electrodes.…”

“…Infections caused by bacteria in livestock, such as bovine tuberculosis, can lead to decreased productivity, trade limitations, and economic difficulties for agricultural producers. Furthermore, the presence of infectious microbes can result in negative consequences for the ecosystem [18]. As an illustration, specific bacterial strains have the potential to pollute water sources, thereby resulting in the emergence of waterborne illnesses.…”

Rapid Detection of SARS-CoV-2 Spike RBD Protein in Body Fluid: Based on Special Calcium Ion-Mediated Gold Nanoparticles Modified by Bromide Ions

“…There is an urgent need to design a fast, simple, ultrasensitive detection platform for biomarker proteins for early diagnosis and treatment . Surface-enhanced Raman spectroscopy (SERS) is a powerful biomolecule recognition technique. , Due to its ultrasensitive, high-precision, noninvasive, trace, and real-time on-site detection, SERS can be developed into a practical technology, especially in toxin and virus detection, environmental science, biosensors, and other broad application prospects. − In addition, Raman fingerprints can be utilized for directly analyzing the structure and function of biomolecules . Currently, SERS is used for detecting numerous proteins, including transferrin, human serum albumin, and human serum transferrin, all biomarkers for different diseases .…”