“…7. This decrease in the gain with increasing in dye concentration beyond optimum concentrations (1 Â 10 À4 , 1Â 10 À4 , 8 Â 10 À2 mol/L for fluorescein in BMIM AlCl 4 , BMIM BF 4 and EtOH) respectively because of the dye molecules quenching and aggregation [41][42][43]. In case of BMIM Cl, fluorescence intensity of fluorescein dye increased gradually with increasing dye concentration till 1 Â 10 À2 mol/L without getting optimum concentration so, its optimum gain may be at concentration higher than 1 Â 10 À2 mol/L.…”