Tuning a pyrazoline‐based fluorogenic reagent, 3‐naphthyl‐1‐(4‐trifluoromethyl)‐5‐(4‐carboxy phenyl)‐2‐pyrazoline for sensitive precolumn derivatization of aliphatic alcohols and trace analysis by RP‐HPLC with fluorescence detection
Abstract:A new member of the pyrazoline family, 3‐naphthyl‐1‐(4‐trifluoromethyl)‐5‐(4‐carboxy phenyl)‐2‐pyrazoline has been evaluated as a precolumn derivatization reagent for the analysis of primary alcohols using HPLC. The simultaneous separation of eight alcohol derivatives (C1–C8) within 15 min was achieved on a reverse‐phase C8 column with an isocratic elution mode. The derivatives were detected with fluorescence at an emission wavelength of 470 nm when excited at 360 nm. The identification of the corresponding de… Show more
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