Tunable metacrylated silk fibroin-based hybrid bioinks for the bioprinting of tissue engineering scaffolds

Jin, Yang; Li, Zhihui; Li, Shikai; Zhang, Qianqian; Zhou, Xiaojun; He, Chuanglong

doi:10.1039/d2bm01978g

Cited by 14 publications

(11 citation statements)

References 48 publications

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“…Mechanical properties of bioink wherein cells are embedded are an important factor for cell viability and proliferation. For this reason, the compressive modulus of crosslinked samples of fibrinogen-based biomaterial ink was calculated, and the mechanical behavior was comparable with other bioinks for skin tissue engineering or soft tissue bioprinting described in the literature [ 40 , 41 , 42 ]. Moreover, the fibrin construct can be easily handled without losing its integrity.…”

Section: Discussionmentioning

confidence: 62%

Fibrinogen-Based Bioink for Application in Skin Equivalent 3D Bioprinting

Cavallo,

Al Kayal,

Mero

et al. 2023

JFB

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Three-dimensional bioprinting has emerged as an attractive technology due to its ability to mimic native tissue architecture using different cell types and biomaterials. Nowadays, cell-laden bioink development or skin tissue equivalents are still at an early stage. The aim of the study is to propose a bioink to be used in skin bioprinting based on a blend of fibrinogen and alginate to form a hydrogel by enzymatic polymerization with thrombin and by ionic crosslinking with divalent calcium ions. The biomaterial ink formulation, composed of 30 mg/mL of fibrinogen, 6% of alginate, and 25 mM of CaCl2, was characterized in terms of homogeneity, rheological properties, printability, mechanical properties, degradation rate, water uptake, and biocompatibility by the indirect method using L929 mouse fibroblasts. The proposed bioink is a homogeneous blend with a shear thinning behavior, excellent printability, adequate mechanical stiffness, porosity, biodegradability, and water uptake, and it is in vitro biocompatible. The fibrinogen-based bioink was used for the 3D bioprinting of the dermal layer of the skin equivalent. Three different normal human dermal fibroblast (NHDF) densities were tested, and better results in terms of viability, spreading, and proliferation were obtained with 4 × 106 cell/mL. The skin equivalent was bioprinted, adding human keratinocytes (HaCaT) through bioprinting on the top surface of the dermal layer. A skin equivalent stained by live/dead and histological analysis immediately after printing and at days 7 and 14 of culture showed a tissuelike structure with two distinct layers characterized by the presence of viable and proliferating cells. This bioprinted skin equivalent showed a similar native skin architecture, paving the way for its use as a skin substitute for wound healing applications.

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Section: Discussionmentioning

confidence: 62%

Fibrinogen-Based Bioink for Application in Skin Equivalent 3D Bioprinting

Cavallo,

Al Kayal,

Mero

et al. 2023

JFB

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“…Yang et al developed a hybrid cell-laden bioink with GelMA and methacrylated silk fibroin (SFMA) for extrusion bioprinting with excellent rheological properties at low temperatures. 351 Since both components showed photo-cross-linking properties via the dual cross-linking mechanism between hydrogel networks, the mechanical properties and shape integrity were synergistically improved compared to the singlecomponent hydrogel system. The PC12 and HUVECs-laden GelMA/SFMA bioprinted scaffolds were implanted in the subcutaneous tissue of the SD rat model for 30 days.…”

Section: Silk Fibroinmentioning

confidence: 99%

“…After 16 days, multinucleated myotubes organized into compact muscle fibers within 4–6 layer grids, highlighting the potential of this approach for cultured meat production. Yang et al developed a hybrid cell-laden bioink with GelMA and methacrylated silk fibroin (SFMA) for extrusion bioprinting with excellent rheological properties at low temperatures . Since both components showed photo-cross-linking properties via the dual cross-linking mechanism between hydrogel networks, the mechanical properties and shape integrity were synergistically improved compared to the single-component hydrogel system.…”

Section: Modulating Scaffold Properties Through Gelma Ink Modificationsmentioning

confidence: 99%

Gelatin Methacryloyl (GelMA)-Based Biomaterial Inks: Process Science for 3D/4D Printing and Current Status

Das,

Jegadeesan,

Basu

2024

Biomacromolecules

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Tissue engineering for injured tissue replacement and regeneration has been a subject of investigation over the last 30 years, and there has been considerable interest in using additive manufacturing to achieve these goals. Despite such efforts, many key questions remain unanswered, particularly in the area of biomaterial selection for these applications as well as quantitative understanding of the process science. The strategic utilization of biological macromolecules provides a versatile approach to meet diverse requirements in 3D printing, such as printability, buildability, and biocompatibility. These molecules play a pivotal role in both physical and chemical cross-linking processes throughout the biofabrication, contributing significantly to the overall success of the 3D printing process. Among the several bioprintable materials, gelatin methacryloyl (GelMA) has been widely utilized for diverse tissue engineering applications, with some degree of success. In this context, this review will discuss the key bioengineering approaches to identify the gelation and cross-linking strategies that are appropriate to control the rheology, printability, and buildability of biomaterial inks. This review will focus on the GelMA as the structural (scaffold) biomaterial for different tissues and as a potential carrier vehicle for the transport of living cells as well as their maintenance and viability in the physiological system. Recognizing the importance of printability toward shape fidelity and biophysical properties, a major focus in this review has been to discuss the qualitative and quantitative impact of the key factors, including microrheological, viscoelastic, gelation, shear thinning properties of biomaterial inks, and printing parameters, in particular, reference to 3D extrusion printing of GelMA-based biomaterial inks. Specifically, we emphasize the different possibilities to regulate mechanical, swelling, biodegradation, and cellular functionalities of GelMA-based bio(material) inks, by hybridization techniques, including different synthetic and natural biopolymers, inorganic nanofillers, and microcarriers. At the close, the potential possibility of the integration of experimental data sets and artificial intelligence/machine learning approaches is emphasized to predict the printability, shape fidelity, or biophysical properties of GelMA bio(material) inks for clinically relevant tissues.

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“…Bioprinting, or the use of 3D printing technologies in concert with bioinks containing bioactive components, is a common technique utilized in tissue engineering due to its ability to create repeatable hierarchical structures that mimic physiological tissue [26][27][28]. The use of controlled release systems within 3D printed scaffolds has the capacity to further increase the relevance of the biochemical environment of the scaffolds [29]; however, the release kinetics of the particles may be influenced by the forces imposed on the particles during printing (for example, the shear forces imposed by extrusion through a needle), and diffusion through the scaffolding material may limit the bioactivity of the released growth factor [30][31][32]. Crosslinking of the biomaterial or hydrogel ink itself may also affect the release kinetics and the ability of loaded growth factors to diffuse through the scaffold, affecting the growth factor's interactions with incorporated cells.…”

Section: Introductionmentioning

confidence: 99%

Development of a Nanoparticle System for Controlled Release in Bioprinted Respiratory Scaffolds

Zimmerling,

Sunil,

Zhou

et al. 2024

JFB

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The use of nanoparticle systems for the controlled release of growth factors is a promising approach to mimicking of the biochemical environment of native tissues in tissue engineering. However, sustaining growth factor release inside an appropriate therapeutic window is a challenge, particularly in bioprinted scaffolds. In this study, a chitosan-coated alginate-based nanoparticle system loaded with hepatocyte growth factor was developed and then incorporated into bioprinted scaffolds. The release kinetics were investigated with a focus on identifying the impact of the chitosan coating and culture conditions. Our results demonstrated that the chitosan coating decreased the release rate and lessened the initial burst release, while culturing in dynamic conditions had no significant impact compared to static conditions. The nanoparticles were then incorporated into bioinks at various concentrations, and scaffolds with a three-dimensional (3D) structure were bioprinted from the bioinks containing human pulmonary fibroblasts and bronchial epithelial cells to investigate the potential use of a controlled release system in respiratory tissue engineering. It was found that the bioink loaded with a concentration of 4 µg/mL of nanoparticles had better printability compared to other concentrations, while the mechanical stability of the scaffolds was maintained over a 14-day culture period. The examination of the incorporated cells demonstrated a high degree of viability and proliferation with visualization of the beginning of an epithelial barrier layer. Taken together, this study demonstrates that a chitosan-coated alginate-based nanoparticle system allows the sustained release of growth factors in bioprinted respiratory tissue scaffolds.

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Tunable metacrylated silk fibroin-based hybrid bioinks for the bioprinting of tissue engineering scaffolds

Abstract: Three-dimensional (3D) bioprinting is a powerful technique for the production of tissue-like structures to study cell behavior and tissue properties. A major challenge in 3D extrusion bioprinting is the limited...

Cited by 14 publications

References 48 publications

Fibrinogen-Based Bioink for Application in Skin Equivalent 3D Bioprinting

Fibrinogen-Based Bioink for Application in Skin Equivalent 3D Bioprinting

Gelatin Methacryloyl (GelMA)-Based Biomaterial Inks: Process Science for 3D/4D Printing and Current Status

Development of a Nanoparticle System for Controlled Release in Bioprinted Respiratory Scaffolds

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