Tumour metastasis in mice with reduced immune reactivity. I. Studies with two MCA‐induced sarcomas in radiation and thymectomized radiation C57BL/6J chimeras

Abstract

Spontaneous metastasis formation from two MCA-induced sarcomas with diferent degrees of antigenicity was studied in radiation and thymectomized radiationTumour-specific immune responses are known to be able to influence the growth of transplanted tumours (Prehn and Main, 1957;Klein et al., 1960). The influence of immunologic factors on the formation of metastases has also been described. Treatment with heterologous antilymphocyte serum (ALS) has increased metastasis formation in allogeneic systems (Hellman et al., 1968;Deodhar and Crile, 1969;Gershon and Carter, 1970) and in syngeneic systems (Fisher et al., 1969). Crile and Deodhar (1971) demonstrated significant protection against metastasis formation from Sarcoma 241 in C57BL mice which were specifically immunized against the tumour.The cited literature implies an inhibitory immunological influence on the formation of metastases. The relative importance of this influence is not clear, however. The net influence of the immune response on tumour dissemination could be elucidated by studying the effects of a defined high degree of immune suppression.Thus, the aim of the present investigation was to study this problem by comparing tumour spread in intact animals with that in animals with deeply depressed immune reactivity. MATERIAL AND METHODS

Herpes Simplex Virus Infection of Mouse Neuroblastoma Cells

“…Cells. The following cells lines were used: clone 41 A3 of mouse neuroblastoma cells C 1300 (11),* mouse L 929 cells, MCG 101 mouse sarcoma cells from a methylcholantrene-induced tumor in C 57 black 6 J mice (12), primary embryonic mouse kidney and brain cells, human glioma cells 138 MG (13), human embryonic lung cells, and…”

Tumour metastasis in mice with reduced immune reactivity. II. Studies with a highly antigenic MCA‐induced sarcoma in thymectomized and/or sub‐lethally irradiated C57BL/6J mice

“…bone-marrow substituted chimeras, n o change in the spread of sarcoma MCGlOl could be demonstrated (Boeryd and Suurkula, 1973). In the present study, by using sub-lethal instead of lethal irradiation, an increased formation of metastasis was noted but only in those groups in which the immunoreactivity had not recovered.…”

“…Subcutaneous transplantation of lo2 cells produced 100% takes. After immunization even lo7 tumour cells did not yield 100% takes (Boeryd and Suurkula, 1973).…”

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Spontaneous metastasis formation was studied in thymectornized andlor sublethally irradiated syngeneic C57BL/6/ mice of a highly antigenic MCA-induced The possible influence of immune reactions on spontaneous metastasis formation of two MCAinduced sarcomas was previously tested in bonemarrow-substituted radiation and thymectomized radiation chimeras of C57BL/6J mice (Boeryd and Suurkula, 1973). No effect on the spread of the sarcomas was noted.

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“…The possible influence of immune reactions on spontaneous metastasis formation of two MCAinduced sarcomas was previously tested in bonemarrow-substituted radiation and thymectomized radiation chimeras of C57BL/6J mice (Boeryd and Suurkula, 1973). No effect on the spread of the sarcomas was noted.…”