Tumor type‐specific differences in cell‐substrate adhesion among human tumor cell lines

Abstract

Cell lines derived from human tumors of 4 different histological types (squamous carcinomas, melanomas, gliomas and a fibrosarcoma) were examined for cell-substrate adhesion on plastic culture dishes and dishes coated with 50 micrograms of type-IV collagen. In the absence of exogenous adhesion factors, the squamous carcinoma cells attached and spread more rapidly than the other cells on both substrates. Once attached, the squamous carcinoma cells were also more difficult than the other cells to remove with proteolytic enzymes/EDTA. While the cell lines derived from melanomas, gliomas and the fibrosarcoma were less adhesive than the squamous carcinoma lines in the absence of exogenous adhesion factors, these cells were highly responsive to laminin. In contrast, laminin only slightly enhanced the attachment and spreading of squamous carcinoma cells on the plastic dishes and actually inhibited attachment and spreading on the collagen-coated dishes. These results indicate that there are tumor-type-specific differences in adhesiveness among human tumor cell lines and that cells from different tumor types may have distinct mechanisms for carrying out one of the functions critical to invasion.

Difference in Laminin Expression Between High and Low Metastatic Cell Clones Derived from Murine Lewis Lung Carcinoma.

“…The present results suggest that LM60-D6 may possess more free (unoccupied by endogenous LN) LNRthan P29 and consequently LM60-D6 may be able to bind to LN in basement membranes better than P29, resulting in the higher metastatic potential. This possibility appears to be pertinent to the previous data which indicate that self-synthesized LN is partially responsible for the competitive inhibition of cell attachment with exogenous LN (2,4,6,16). As shown in Figure 3, neither LM60-D6nor P29 expresses the gene of the LNA chain.…”

“…In some cell lines, e.g., murine BL6 from B16 melanoma cell and PM2from PMTfibrosarcoma cell, it has been shown that LNpromotes attachment of metastatic tumor cell to type IV collagen (14). However, in murine macrophage-derived M5706 cell (2), murine glioma cell (4) and human squamous carcinoma cell (16), LNinhibits the attachment of the cells to type IV collagen.…”

The Role of Laminin in Cancer Invasion and Metastasis

“…LN does not affect the attachment of the poorly metastatic FI variant while fibronectin tends to increase its attachment [7], How ever, these findings may not be applicable to human melanoma. Although LN stimulates the attachment of human melanoma cells to tissue culture plastic and type IV collagen [8], Ormerod et al [9] could not demonstrate that the metastatic behaviour of a series of human melanoma cell lines correlates with their abilities to use LN to attach to type IV collagen.…”

Modulation of Squamous Carcinoma Cell Growth, Morphology, Adhesiveness and Extracellular Matrix Production by Interferon-γ and Tumor Necrosis Factor-α

“…Cells were grown at 37 °C and 5% CO2 and sub cultured by trypsinization as required. The isolation and character ization of these lines have been described in previous reports [ 14] and several of these lines have been used by us in recent studies [15][16][17],…”