The Role of Laminin in Cancer Invasion and Metastasis

Hunt, Gillian

doi:10.1159/000163521

Cited by 30 publications

(29 citation statements)

References 22 publications

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“…The tethering was partially mediated by α6β1 and α6β4, while the spreading was mostly by α2β1 and α6β1 integrins. Although LM has been proposed to have a strong association with the metastatic potential of various cancers (Hunt, 1989;Liotta, 1991;Honn and Tang, 1992), our study further emphasizes the role of this molecule in tumour metastasis. This interaction seems to be especially important when the metastasis develops in EC-damaged areas or in organs with LM-rich vessel system.…”

Section: Discussionsupporting

confidence: 44%

Laminin mediates tethering and spreading of colon cancer cells in physiological shear flow

et al. 1999

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Summary Under the physiological shear condition, cultured colon cancer cells bound to laminin (LM), but not to fibronectin or vitronectin. Most of the tethered cells did not roll, but arrested immediately and spread within 10-30 min on LM under the continuous presence of shear flow. The tethering of Colo201 was partially inhibited by monoclonal antibodies (mAbs) to α6 integrin and a combination of mAbs to β1 and β4 integrins, but not by mAb to 67KD laminin receptor. Some Colo201 cells still tethered at 4°C. This suggests that α6β1 and α6β4 integrins participate in Colo201 tethering on LM, although other non-integrin molecules play roles. In contrast, the spread of Colo201 was effectively inhibited by the mAbs to integrin α2, α6 and β1 chains. The effect of anti-α2 plus anti-α6 mAbs was almost equal to anti-β1, suggesting that Colo201 cells mainly use α2β1 and α6β1 integrins for spreading on LM. When the cells were perfused on subconfluent endothelial cells (HUVEC) cultured on LM, they did not tether on HUVEC but did on coated LM exposed at intercellular gap area. Immunohistochemistry revealed that LM abundantly existed in the cytosol of human portal and hepatic vein endothelial cells. These data suggest that LM can mediate from tethering to spreading of colon cancer cells under the blood flow and plays an essential role in haematogeneous metastasis.

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Section: Discussionsupporting

confidence: 44%

Laminin mediates tethering and spreading of colon cancer cells in physiological shear flow

et al. 1999

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“…We also examined the presence of laminin-1 and fibronectin in carcinoma cell-associated stroma in 35 ovary carcinoma samples by immunohistochemistry, because it is known that cancer cells express and secrete these molecules (Christensen, 1992;Hunt, 1989). The majority of the cases showed conspicuous colocalization of gal-1, laminin-1, and fibronectin (Fig.…”

Section: Colocalization Of Galectin-1 Laminin-1 and Fibronectin In mentioning

confidence: 99%

Galectin-1 Accumulation in the Ovary Carcinoma Peritumoral Stroma Is Induced by Ovary Carcinoma Cells and Affects Both Cancer Cell Proliferation and Adhesion to Laminin-1 and Fibronectin

Brûle

Califice

Garnier

et al. 2003

Laboratory Investigation

154

106

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SUMMARY:Galectin-1 (gal-1) is a 14-kDa laminin-binding galectin involved in several biologic events including regulation of cancer cell proliferation and adhesion to the matrix. In this study, we examined gal-1 expression in 30 human epithelial ovary carcinoma samples by Western and Northern blotting and by immunohistochemistry. Gal-1 mRNA levels were increased in more than 95% of the examined ovary carcinoma samples, compared with a wedge resection of a normal ovary.Immunohistochemical analysis of the samples demonstrated gal-1 expression in cancer epithelial cells from 17 of 30 samples, with a cytoplasmic pattern. Gal-1 immunostaining was significantly increased in the stroma associated with carcinoma cells compared with the normal, noninvaded stroma (p ϭ 0.003). This pattern of expression was confirmed by examination of 12 other frozen epithelial ovary carcinomas, using in situ hybridization. Immunohistochemical staining of the specimens demonstrated colocalization of gal-1, laminin-1, and fibronectin. In vitro experiments were conducted to elucidate the potential biologic role of gal-1 in ovarian cancer progression. Gal-1 protein expression and release was detected in AZ364, SK-OV-3, and AZ224, but not in OVCAR-3, AZ419, and AZ382, human ovary carcinoma cell lines. Incubation of 84BR fibroblasts with conditioned media harvested from the ovary carcinoma cell lines induced an increased expression of gal-1 in the cultured fibroblasts in all cases except AZ419 and SK-OV-3. High concentrations of gal-1 (100 g/ml) induced significantly decreased cell proliferation in all cell lines, as defined by bromodeoxyuridine incorporation. Additionally, recombinant gal-1 induced a dose-dependent increase in in vitro adhesion of AZ224, SK-OV-3, and AZ382 cells to laminin-1; adhesion to fibronectin was increased by gal-1 in OVCAR-3, AZ224, and SK-OV-3. No effect was observed in the other cases. Our data contribute to define a role for gal-1 during the interactions between human ovary carcinoma cells and host fibroblasts. (Lab Invest 2003, 83:377-386).

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“…SCLC is a rapidly proliferating neoplasm with a marked tendency toward early metastatic spread. It has been shown that laminin enhances metastases formation of tumour cells (Terranova et al, 1983;Barsky et al, 1984;Hunt, 1989). However, the value of serum laminin P1 for indicator of distal metastasis in SCLC is disappointing.…”

Section: Discussionmentioning

confidence: 99%

Serum laminin P1 in small cell lung cancer: A valuable indicator of distant metastasis?

1993

Lung Cancer

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Summary Serum laminin P1 was studied in patients with small cell lung cancer (SCLC), non-small cell lung cancer (NSCLC), respiratory infections, pulmonary fibrosis, and in normal subjects. The level of serum laminin P1 was elevated (> 1.27 U ml-') in 58.9% of SCLC and in 11.5% of NSCLC patients. Median value in SCLC was significantly higher than that in NSCLC (P<0.01), respiratory infection (P<0.01), and in normal subjects (P<0.01), but not statistically different from that in pulmonary fibrosis. The levels of serum laminin P1 in SCLC were related to therapeutic response. However, no certain correlation was established between the level of laminin P1 and the clinical stage of SCLC.

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The Role of Laminin in Cancer Invasion and Metastasis

Cited by 30 publications

References 22 publications

Laminin mediates tethering and spreading of colon cancer cells in physiological shear flow

Laminin mediates tethering and spreading of colon cancer cells in physiological shear flow

Galectin-1 Accumulation in the Ovary Carcinoma Peritumoral Stroma Is Induced by Ovary Carcinoma Cells and Affects Both Cancer Cell Proliferation and Adhesion to Laminin-1 and Fibronectin

Serum laminin P1 in small cell lung cancer: A valuable indicator of distant metastasis?

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