Tumor-targeted liposomes: doxorubicin-loaded long-circulating liposomes modified with anti-cancer antibody

Lukyanov, Anatoly N.; Elbayoumi, Tamer; Chakilam, Ananthsrinivas; Torchilin, Vladimir P.

doi:10.1016/j.jconrel.2004.08.007

Cited by 266 publications

(203 citation statements)

References 26 publications

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“…The NMR ( 1 H-NMR, 13 C-NMR, 31 P-NMR) was recorded on an NMR spectrometer (Bruker AVANCE 400, Ettlingen,Baden-Württemberg, Germany). During the measurement, HP-$-CD was dissolved in dimethyl sulfoxide (DMSO)-d6 and HP-$-CD-PLA-DPPE copolymers were dissolved in CdCl 3 .…”

Section: Characterization Of the Chemical Structure Of Hp-β-cd And Hpmentioning

confidence: 99%

“…Compared with HP-$-CD (Fig. 2C), the 13 C NMR spectra of the HP-$-CD-PLA-DPPE copolymer (Fig. 2D) showed that the peak at ∼9.0 ppm was attributed to the −CH 3 group carbon peak of the DPPE moiety located at the terminal groups.…”

Section: Synthesis and Characterization Of Hp-β-cd-pla-dppe Copolymersmentioning

confidence: 99%

“…[9][10][11] The characteristic size, stability, and the longevity in the systemic circulation make PEG-PE micelles a promising carrier for the delivery of drugs to the ill site via the EPR effect. 12,13 But the PEG-PE copolymers are expensive and this will hinder its further applications.…”

Section: Introductionmentioning

confidence: 99%

“…The copolymers consisted of hydrophilic, HP-$-CD, segment and hydrophobic, PLA and DPPE, segment. The copolymers were characterized on the basis of FT-IR, 1 H NMR, 13 C NMR, and 31 P NMR measurements. The DOX-loaded copolymer NPs were fabricated by double emulsion (DE) and nanoprecipitation (NP) methods.…”

Section: Introductionmentioning

confidence: 99%

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Hydroxypropyl-β-Cyclodextrin Copolymers and Their Nanoparticles as Doxorubicin Delivery System

Wang

Zhang

Liang

et al. 2011

Journal of Pharmaceutical Sciences

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Section: Characterization Of the Chemical Structure Of Hp-β-cd And Hpmentioning

confidence: 99%

Section: Synthesis and Characterization Of Hp-β-cd-pla-dppe Copolymersmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Hydroxypropyl-β-Cyclodextrin Copolymers and Their Nanoparticles as Doxorubicin Delivery System

Wang

Zhang

Liang

et al. 2011

Journal of Pharmaceutical Sciences

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“…Liposomal delivery has been shown to reduce cardiac toxicity of doxorubicin (Perez et al, 2002). Targeted liposomes can be synthesized by incorporating a tumor cell-selective ligand, such as antibodies, transferrin and folic acid (Gabizon et al, 2004;Lukyanov et al, 2004;Pan et al 2007;Wu et al, 2006a;Xiong et al, 2005), via a lipophilic anchor, typically consisting of a derivative of distearoylphosphatidylethanolamine (DSPE). …”

mentioning

confidence: 99%

Synthesis and evaluation of a novel ligand for folate-mediated targeting liposomes

Xiang

et al. 2008

International Journal of Pharmaceutics

112

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Folate receptors (FRs) have been identified as cellular surface markers for cancer and leukemia. Liposomes containing lipophilic derivatives of folate have been shown to effectively target FRexpressing cells. Here, we report the synthesis of a novel lipophilic folate derivative, folatepolyethylene glycol-cholesterol hemisuccinate (F-PEG-CHEMS), and its evaluation as a targeting ligand for liposomal doxorubicin (L-DOX) in FR-expressing cells. Liposomes containing F-PEG-CHEMS, with a mean diameter of 120±20nm, were synthesized by polycarbonate membrane extrusion and were shown to have excellent colloidal stability. The liposomes were taken up selectively by KB cells, which overexpress FR-α. Compared to folate-PEG-cholesterol (F-PEGChol), which contains a carbamate linkage, F-PEG-CHEMS better retained its FR-targeting activity during prolonged storage. In addition, F-PEG-CHEMS containing liposomes loaded with DOX (F-L-DOX) showed greater cytotoxicity (IC 50 =10.0μM) than non-targeted control L-DOX (IC 50 = 57.5μM) in KB cells. In ICR mice, both targeted and non-targeted liposomes exhibited long circulation properties, although F-L-DOX (t 1/2 = 12.34 hr) showed more rapid plasma clearance than L-DOX (t 1/2 = 17.10 hr). These results suggest that F-PEG-CHEMS is effective as a novel ligand for the synthesis of FR-targeted liposomes. Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. , 2001; Momot et al., 2003;Papahadjopoulos et al., 1991) and is facilitated by plasma protein opsonization. Incorporation of polyethylene glycol (PEG) coating on liposomes has been shown to reduce the rate of RES clearance of liposomes Fritze et al., 2006; Momot et al., 2003;Papahadjopoulos et al., 1991). Liposomal delivery has been shown to reduce cardiac toxicity of doxorubicin (Perez et al., 2002). Targeted liposomes can be synthesized by incorporating a tumor cell-selective ligand, such as antibodies, transferrin and folic acid (Gabizon et al., 2004;Lukyanov et al., 2004;Pan et al. 2007;Wu et al., 2006a;Xiong et al., 2005), via a lipophilic anchor, typically consisting of a derivative of distearoylphosphatidylethanolamine (DSPE). NIH Public AccessMembrane folate receptors (FRs), including FR-α and FR-β, are glycosylphosphatidylinositol (GPI)-anchored glycoproteins. FR-α expression is amplified in over 90% of ovarian carcinomas and at varying frequencies in other epithelial cancers. Meanwhile, FR-β is expressed in a non-functional form in neutrophils and in a functional form in activated macrophages and in myeloid leukemias (Elwood et al., 1989;McHugh et al., 1979). In contrast, most normal...

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Current Status and Future Prospects for Nanoparticle‐Based Technology in Human Medicine

Sanvicens

Fernández

Salvador

et al. 2011

Nanoplatform‐Based Molecular Imaging

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Tumor-targeted liposomes: doxorubicin-loaded long-circulating liposomes modified with anti-cancer antibody

Cited by 266 publications

References 26 publications

Hydroxypropyl-β-Cyclodextrin Copolymers and Their Nanoparticles as Doxorubicin Delivery System

Hydroxypropyl-β-Cyclodextrin Copolymers and Their Nanoparticles as Doxorubicin Delivery System

Synthesis and evaluation of a novel ligand for folate-mediated targeting liposomes

Current Status and Future Prospects for Nanoparticle‐Based Technology in Human Medicine

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