Tumor Spheroids as Model to Design Acoustically Mediated Drug Therapies: A Review

Roy, Marie Le; Alix, Corentin; Bouakaz, Ayache; Sérrière, Sophie; Escoffre, Jean‐Michel

doi:10.3390/pharmaceutics15030806

Cited by 8 publications

(8 citation statements)

References 111 publications

(155 reference statements)

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“…Among these 3D models, tumor spheroids are the most exploited for the design and validation of anticancer strategies. As reported by Roy et al, tumor spheroids are 3D aggregates of tumor cells that may or may not be associated with other cell types, such as fibroblasts or immune cells. These cells interact not only with themselves but also with an extracellular matrix ( i.e.…”

Section: Introductionmentioning

confidence: 90%

“…14 Since the advent of spheroids, fewer than ten published in vitro studies have used spheroids to either design and evaluate US protocols and new formulations of drugs and MBs or to investigate the influence of the tumor microenvironment on therapeutic efficacy. 1,15,16 In this context, the aims of the present study are (i) to evaluate the influence of acoustic pressure and MB concentration on the delivery of a drug model into colorectal cancer (CRC) spheroids and (ii) to investigate whether MB-assisted US is able to deliver different types of chemotherapeutic drugs in these CRC spheroids.…”

Section: ■ Introductionmentioning

confidence: 99%

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Delivery of Anticancer Drugs Using Microbubble-Assisted Ultrasound in a 3D Spheroid Model

Roy,

Alix,

Burlaud-Gaillard

et al. 2024

Mol. Pharmaceutics

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Tumor spheroids are promising three-dimensional (3D) in vitro tumor models for the evaluation of drug delivery methods. The design of noninvasive and targeted drug methods is required to improve the intratumoral bioavailability of chemotherapeutic drugs and reduce their adverse off-target effects. Among such methods, microbubble-assisted ultrasound (MB-assisted US) is an innovative modality for noninvasive targeted drug delivery. The aim of the present study is to evaluate the efficacy of this US modality for the delivery of bleomycin, doxorubicin, and irinotecan in colorectal cancer (CRC) spheroids. MB-assisted US permeabilized the CRC spheroids to propidium iodide, which was used as a drug model without affecting their growth and viability. Histological analysis and electron microscopy revealed that MB-assisted US affected only the peripheral layer of the CRC spheroids. The acoustically mediated bleomycin delivery induced a significant decrease in CRC spheroid growth in comparison to spheroids treated with bleomycin alone. However, this US modality did not improve the therapeutic efficacy of doxorubicin and irinotecan on CRC spheroids. In conclusion, this study demonstrates that tumor spheroids are a relevant approach to evaluate the efficacy of MB-assisted US for the delivery of chemotherapeutics.

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Section: Introductionmentioning

confidence: 90%

Section: ■ Introductionmentioning

confidence: 99%

Delivery of Anticancer Drugs Using Microbubble-Assisted Ultrasound in a 3D Spheroid Model

Roy,

Alix,

Burlaud-Gaillard

et al. 2024

Mol. Pharmaceutics

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“…This makes them highly useful for many applications like as drug screening, drug design, drug targeting, drug toxicity assessment, and validation of drug delivery techniques. 33 Nevertheless, despite the benefits they offer, the use of tumor spheroids in pharmaceutical research is accompanied by many obstacles and restrictions.…”

Section: Challenges and Limitationsmentioning

confidence: 99%

A promising breakthrough in pancreatic cancer research: The potential of spheroids as 3D models

Jamshidi,

Modarresi Chahardehi

et al. 2024

Bioimpacts

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Pancreatic ductal adenocarcinoma (PDAC) stands as the fourth leading cause of cancer-related deaths, primarily attributable to its resistance to chemotherapy, resulting in a nearly universal fatality rate. Despite the promise exhibited by numerous drugs in preclinical studies, their subsequent failure in clinical trials underscores the inherent limitations of conventional two-dimensional cell culture models commonly employed in early drug screening endeavors. The inadequacies of two-dimensional (2D) models prompted the exploration of three-dimensional (3D) culture systems, which more faithfully recapitulate the native tumor microenvironment. These 3D systems have distinct advantages over 2D models in morphology, proliferation, drug response, and protein expression. Among these 3D platforms, tumor organoids and spheroids, generated through different methodologies, have emerged as next-generation models that closely mirror aspects of pancreatic tumor biology. This comprehensive review scrutinizes pancreatic cancer spheroids' techniques, tissue sources, and applications, offering a nuanced analysis of their advantages and limitations. By comparing these distinct 3D culture systems, researchers gain valuable insights to inform the selection of optimal model designs aligned with their specific experimental objectives. The utilization of these advanced models holds significant promise for enhancing the clinical relevance of both in vitro and in vivo cancer research, thereby contributing to the development of improved therapeutics against pancreatic cancer.

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“…Spheroids are cellular aggregates embedded with collagen type I, with outer cells adhering to and invading into the matrix [ 34 ]. These compact, globular structures can mimic diverse microenvironments within tumors, including anoxic, hypoxic, and oxic niches [ 35 ]. Organoids are self-organized three-dimensional structures derived in vitro from pluripotent or adult stem cells [ 9 ].…”

Section: Advance In Os Cells and Modelsmentioning

confidence: 99%

Advances of Osteosarcoma Models for Drug Discovery and Precision Medicine

Tan,

Wang,

et al. 2023

Biomolecules

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The management of osteosarcoma (OS) patients presents a significant clinical challenge. Despite progress in conventional and targeted therapies, the survival rate of OS patients remains limited largely due to therapy resistance and the high metastatic potential of the disease. OS models that accurately reflect the fundamental characteristics are vital to the innovation and validation of effective therapies. This review provides an insight into the advances and challenges in OS drug development, focusing on various preclinical models, including cell lines, 3D culture models, murine models, and canine models. The relevance, strengths, and limitations of each model in OS research are explored. In particular, we highlight a range of potential therapeutics identified through these models. These instances of successful drug development represent promising pathways for personalized OS treatment.

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Tumor Spheroids as Model to Design Acoustically Mediated Drug Therapies: A Review

Cited by 8 publications

References 111 publications

Delivery of Anticancer Drugs Using Microbubble-Assisted Ultrasound in a 3D Spheroid Model

Delivery of Anticancer Drugs Using Microbubble-Assisted Ultrasound in a 3D Spheroid Model

A promising breakthrough in pancreatic cancer research: The potential of spheroids as 3D models

Advances of Osteosarcoma Models for Drug Discovery and Precision Medicine

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