Tumor-on-a-chip model for advancement of anti-cancer nano drug delivery system

Tian, Chutong; Zheng, Shunzhe; Liu, Xinying; Kamei, Kazuhito

doi:10.1186/s12951-022-01552-0

Cited by 23 publications

(19 citation statements)

References 115 publications

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“…Ideally, in vivo effects should be predicted by in vitro experiments: thus, we aimed to establish a system which enabled the investigation of the anti-tumor efficacy of magnetically accumulated particles or immune cells against spheroids. Hitherto, others had performed investigations on tumor spheroids with nanoparticles under flow conditions, but without magnetic fields [ 49 , 50 ]. We had already performed a pilot study employing colon carcinoma spheroids placed in hand-made agarose beds in Ibidi µ-slides [ 17 ].…”

Section: Discussionmentioning

confidence: 99%

In Vitro Setup for Determination of Nanoparticle-Mediated Magnetic Cell and Drug Accumulation in Tumor Spheroids under Flow Conditions

Behr

Carnell

Stein

et al. 2022

Cancers

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Superparamagnetic iron oxide nanoparticles (SPIONs) are used in nanomedicine as transporter systems for therapeutic cargos, or to magnetize cells to make them magnetically guidable. In cancer treatment, the site-directed delivery of chemotherapeutics or immune effector cells to the tumor can increase the therapeutic efficacy in the target region, and simultaneously reduce toxic side-effects in the rest of the body. To enable the transfer of new methods, such as the nanoparticle-mediated transport from bench to bedside, suitable experimental setups must be developed. In vivo, the SPIONs or SPION-loaded cells must be applied into the blood stream, to finally reach the tumor: consequently, targeting and treatment efficacy should be analyzed under conditions which are as close to in vivo as possible. Here, we established an in vitro method, including tumor spheroids placed in a chamber system under the influence of a magnetic field, and adapted to a peristaltic pump, to mimic the blood flow. This enabled us to analyze the magnetic capture and antitumor effects of magnetically targeted mitoxantrone and immune cells under dynamic conditions. We showed that the magnetic nanoparticle-mediated accumulation increased the anti-tumor effects, and reduced the unspecific distribution of both mitoxantrone and cells. Especially for nanomedical research, investigation of the site-specific targeting of particles, cells or drugs under circulation is important. We conclude that our in vitro setup improves the screening process of nanomedical candidates for cancer treatment.

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Section: Discussionmentioning

confidence: 99%

In Vitro Setup for Determination of Nanoparticle-Mediated Magnetic Cell and Drug Accumulation in Tumor Spheroids under Flow Conditions

Behr

Carnell

Stein

et al. 2022

Cancers

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“…Third, significant differences exist between animal models and humans [107,108], and further research on the correlations between them is required. Advances in technology such as organ-on-a-chip or organoids may be promising for evaluating these complicated synergistic strategies [109][110][111]. In addition, most studies have focused on the combined influence of PDT and ferroptosis on intracellular redox homeostasis.…”

Section: Discussionmentioning

confidence: 99%

Recent progress in cancer therapy based on the combination of ferroptosis with photodynamic therapy

Zeping

Zheng

Kamei

et al. 2022

Acta Materia Medica

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Current anticancer treatments have many limitations to achieving high efficacy. Hence, novel strategies that broaden therapeutic prospects must urgently be developed. Ferroptosis is an iron-dependent form of non-apoptotic programmed cell death that is induced by cellular antioxidative system inhibition. Photodynamic therapy (PDT) uses photosensitizers to generate reactive oxygen species and aggravate oxidative stress in tumor cells. Combining ferroptosis with PDT cooperatively regulates intracellular redox homeostasis, thus increasing cancer cell susceptibility to oxidative stress and yielding synergistic anticancer effects. In this review, various strategies for combining ferroptosis with PDT are comprehensively summarized and discussed, including mono-PDT and PDT-induced ferroptosis, combining PDT with small-molecule ferroptosis inducers, and combining PDT with metal-ion-induced ferroptosis. Additionally, the possibility of combining ferroptosis and PDT with other anti-tumor therapies is discussed. Finally, the prospects and challenges of combining ferroptosis with PDT in clinical cancer treatment are addressed. With increased understanding of the superiority of combination PDT with ferroptosis for cancer treatment, we hope that drug delivery systems based on this strategy will be further developed to increase anticancer efficiency and achieve successful clinical translation.

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“…[ 108 ] Microfluidics‐based tumor‐on‐a‐chip systems can replicate advanced in vivo tumor features at a microscale level, such as the tumor microenvironment, 3D tissue structure, and dynamic culture conditions, improving the correlation between preclinical and clinical trial results in evaluating chemotherapeutic nanomedicines. [ 109 ] Meanwhile, the use of magnetic nanoparticle‐based microfluidic chips in conjunction with established procedures allows for rapid, efficient, and early diagnostic resolution in lung cancer. [ 110 ]…”

Section: Application Of Mixing For the Precipitation Of Apismentioning

confidence: 99%

Hydrodynamics and Transport Mechanism of Microfluidic Mixing in Precipitation of Nanodrugs: A Review

Behera

Patel

Parikh

2023

Cryst. Res. Technol.

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Poor aqueous solubility drugs are a concern of pharmaceutical industries due to low dissolution rates and low bioavailability. Various approaches and techniques address these issues by customizing drug micro–nanonization, as investigated by many researchers. Establishing a compelling synthesis of tailored nanoparticles with highly variable qualities intended for therapeutic applications needs the flexibility to vary particle size, content, crystallinity, and shape. Such an approach necessitates getting to the desired yield and developing a synthetic process that is durable, reproducible, and scalable. However, precipitation techniques to produce nanoparticles are more convenient than other approaches. Liquid antisolvent precipitation is one of the robust crystal manipulation approaches extensively used for producing nanoparticles, where mixing sets the basis to create supersaturation followed by corresponding quality precipitation. Various process intensification equipments are in use for adequate mixing‐based precipitation. Nowadays, microfluidic precipitation system is gaining interest in the production of nanodrugs. Further, the use of ultrasound in a microfluidic system improves the mixing quality by cavitation phenomena. This review focuses on the transport phenomena and hydrodynamics involved in mixing to enhance the mass transfer during antisolvent precipitation of synthesized nanodrugs in the microfluidic system.

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Tumor-on-a-chip model for advancement of anti-cancer nano drug delivery system

Cited by 23 publications

References 115 publications

In Vitro Setup for Determination of Nanoparticle-Mediated Magnetic Cell and Drug Accumulation in Tumor Spheroids under Flow Conditions

In Vitro Setup for Determination of Nanoparticle-Mediated Magnetic Cell and Drug Accumulation in Tumor Spheroids under Flow Conditions

Recent progress in cancer therapy based on the combination of ferroptosis with photodynamic therapy

Hydrodynamics and Transport Mechanism of Microfluidic Mixing in Precipitation of Nanodrugs: A Review

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