Tumor Necrosis Factor–α Overexpression in Lung Disease

Abstract

We conclude that TNF-alpha overexpression causes pathologic changes consistent with both emphysema and pulmonary fibrosis combined with a general lung inflammation, and consequently does not model any single human disease. Our study thus confirms the pleiotropic effects of TNF-alpha, which has been implicated in multiple inflammatory disorders, and underscores the necessity of using a wide range of investigative techniques to link gene expression and phenotype in animal models of disease.

Simultaneous Stimulation with TGF-β1 and TNF-α Induces Epithelial Mesenchymal Transition in Bronchial Epithelial Cells

“…Clinical trials showed that anti-TNF-α therapy was effective in severe asthma patients [30,31]. Moreover, TNF-α overexpression in a murine model induced airspace enlargement, the loss of small airspaces, and increased collagen, which are features of COPD [32]. Those reports thus suggested that TNF-α might be involved in airway remodeling.…”

Measurement of tumor necrosis factor-α, leukotriene B4, and interleukin 8 in the exhaled breath condensate in patients with acute exacerbations of chronic obstructive pulmonary disease

“…Our level of TNF-α was lower than that measured by cytometric bead array assay and this might be explained by the different methods of biomarker measurement (Gessner et al 2005). An experimental study with animal models showed that over-expression of TNF-α induced the pathological changes similar to emphysema and pulmonary fibrosis (Lundblad et al 2005). It is likely that TNF-α plays an important role in the pathogenesis of COPD and its level may be altered during the course of AECOPD.…”

Endogenous Distal Airway Progenitor Cells, Lung Mechanics, and Disproportionate Lobar Growth Following Long-Term Postpneumonectomy in Mice

“…Immediately prior to scanning, the lungs were inflated to 3 cm of PEEP with nitrogen to ensure uniform inflation between different mice (43,44). Isosurface renderings were generated as previously described (43).…”

“…Immediately prior to scanning, the lungs were inflated to 3 cm of PEEP with nitrogen to ensure uniform inflation between different mice (43,44). Isosurface renderings were generated as previously described (43). To measure lung volumes, the borders of the parenchymal lung tissue were manually traced on serial coronal sections obtained from the CT scans using Microview visualization software, version 1.2.0-b2 (GE HealthCare, London, ON, Canada) (43,44).…”

“…Isosurface renderings were generated as previously described (43). To measure lung volumes, the borders of the parenchymal lung tissue were manually traced on serial coronal sections obtained from the CT scans using Microview visualization software, version 1.2.0-b2 (GE HealthCare, London, ON, Canada) (43,44). Tracings excluded major airways and blood vessels and were performed by a single operator for consistency.…”