Tuberculosis on the north coast of Peru: skeletal and molecular paleopathology of late pre-Hispanic and postcontact mycobacterial disease

Klaus, Haagen D.; Wilbur, Alicia K.; Temple, Daniel H.; Buikstra, Jane E.; Stone, Anne C.; Fernández, M.C. Gayol; Wester, Carlos; Tam, Manuel E.

doi:10.1016/j.jas.2010.05.019

Cited by 54 publications

(39 citation statements)

References 54 publications

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“…Buikstra, 1981;Klaus et al, 2010;Roberts, 2015). In the Neolithic Old World, skeletons with the infection have been reported in the Near East and North Africa (Ortner, 1979(Ortner, , 1999Strouhal, 1987;El-Najjar et al, 1997;Crubézy et al, 1998;Zias, 1998;Dabernat and Crubézy, 2010), with the potential Israeli evidence dating back to the eighth millennium BC (Hershkovitz et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

A 6500-year-old Middle Neolithic child from Pollera Cave (Liguria, Italy) with probable multifocal osteoarticular tuberculosis

Sparacello

Roberts

Kerudin

et al. 2017

International Journal of Paleopathology

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Citation for published item: p r elloD it le F nd o ertsD gh rlotte eF nd uerudinD emmielle nd w¤ ullerD omy @PHIUA 9e TSHHEye rEold widdle xeolithi hild from oller g ve @viguri D st lyA with pro le multifo l osteo rti ul r tu er ulosisF9D sntern tion l journ l of p leop thologyFD IU F ppF TUEURF Further information on publisher's website: Use policyThe full-text may be used and/or reproduced, and given to third parties in any format or medium, without prior permission or charge, for personal research or study, educational, or not-for-pro t purposes provided that:• a full bibliographic reference is made to the original source • a link is made to the metadata record in DRO • the full-text is not changed in any way The full-text must not be sold in any format or medium without the formal permission of the copyright holders.Please consult the full DRO policy for further details.

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Section: Introductionmentioning

confidence: 99%

A 6500-year-old Middle Neolithic child from Pollera Cave (Liguria, Italy) with probable multifocal osteoarticular tuberculosis

Sparacello

Roberts

Kerudin

et al. 2017

International Journal of Paleopathology

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“…The two remaining samples testing positive for multiple assays in our qPCR screening (15,281) only did so after extraction with a newer protocol [22], which could account for the discrepancy between qPCR and the negative in-solution enrichment results. Re-analysis of earlier qPCR data as well as newly tested extracts from Lambayeque ILL-22, reported positive in reference [76], reveals the absence of IS6110 and other MTBC loci, according to our stricter criteria. Likewise, bone from Chiribaya Alta T-30, originally detected from mummified lung tissues reported in reference [7], was not found to be positive in this study.…”

Section: Resultsmentioning

confidence: 63%

Screening ancient tuberculosis with qPCR: challenges and opportunities

Harkins

Buikstra

Campbell

et al. 2015

Phil. Trans. R. Soc. B

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The field of ancient DNA (aDNA) has rapidly accelerated in recent years as a result of new methods in next-generation sequencing, library preparation and targeted enrichment. Such research is restricted, however, by the highly variable DNA preservation within different tissues, especially when isolating ancient pathogens from human remains. Identifying positive candidate samples via quantitative PCR (qPCR) for downstream procedures can reduce reagent costs, increase capture efficiency and maximize the number of sequencing reads of the target. This study uses four qPCR assays designed to target regions within the Mycobacterium tuberculosis complex (MTBC) to examine 133 human skeletal samples from a wide geographical and temporal range, identified by the presence of skeletal lesions typical of chronic disseminated tuberculosis. Given the inherent challenges working with ancient mycobacteria, strict criteria must be used and primer/probe design continually re-evaluated as new data from bacteria become available. Seven samples tested positive for multiple MTBC loci, supporting them as strong candidates for downstream analyses. Using strict and conservative criteria, qPCR remains a fast and effective screening tool when compared with screening by more expensive sequencing and enrichment technologies.

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“…2). En 10 meses de excavaciones de gran escala y análisis de laboratorio desde 2004, se documentaron diferentes actividades, las fases de construcción y los patrones funerarios en la Capilla (Klaus 2008;Klaus 2013Klaus y Tam 2009a, 2009bKlaus et al 2009;Klaus et al 2010) (Fig. 3).…”

Section: El Contexto Arqueológicounclassified

“…El aumento de PDAM en los dientes anteriores y posteriores también se observa entre los adultos poscontacto. Las muestras pre y poscontacto comparten grados estadísticamente equivalentes de desgaste dental, periodontitis, inflamación apical, lesiones faciales traumáticas y la dureza de la dieta (Klaus y Tam 2010); el riesgo de evulsión del diente de estas fuentes es igual. Por lo tanto, el patrón de PDAM poscontacto es más probable como una función del número mayor de caries dental.…”

Section: Cambios Dietéticosunclassified

Tuberculosis on the north coast of Peru: skeletal and molecular paleopathology of late pre-Hispanic and postcontact mycobacterial disease

Cited by 54 publications

References 54 publications

A 6500-year-old Middle Neolithic child from Pollera Cave (Liguria, Italy) with probable multifocal osteoarticular tuberculosis

A 6500-year-old Middle Neolithic child from Pollera Cave (Liguria, Italy) with probable multifocal osteoarticular tuberculosis

Screening ancient tuberculosis with qPCR: challenges and opportunities

Vida y muerte en el Perú colonial: inicios de la bioarqueología en Lambayeque histórico (1536-1750 d.C.)

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