Tubedown-1, A novel acetyltransferase associated with blood vessel development

Gendron, Robert L.; Adams, Lisa C.; Paradis, Hélène

doi:10.1002/(sici)1097-0177(200006)218:2<300::aid-dvdy5>3.0.co;2-k

Cited by 52 publications

(35 citation statements)

References 47 publications

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“…The 5.5 kb NATH cDNA sequenced therefore represents the longer 5.8 kb mRNA incom- pletely sequenced in the 5'-untranslated region. In contrast, mouse Tbdn-1 mRNA was found to be 3.4 kb (Gendron et al, 2000), while Northern blot of mouse NARG1 mRNA (Sugiura et al, 2001) are consistent with our data. At the protein level, mouse Tbdn-1 was reported to be 69 kDa, suggested to result from an alternative translation initiation predicted to encode a protein of 594 amino acids.…”

Section: Discussionsupporting

confidence: 90%

“…These data, and the relative overexpression primarily in aggressive and dedifferentiated thyroid carcinomas suggest that NATH may be involved in cellular proliferation at some level. Interestingly, mouse tbdn-1 was expressed in M1 myeloid leukemia cells and was down-regulated during LIF-induced differentiation (Gendron et al, 2000), while mouse NARG-1 (mNAT1) was up-regulated in neonatal brain regions with neuronal proliferation, and further down-regulated by NMDA receptor activation (Sugiura et al, 2001). However, our NATH transfection experiments of papillary thyroid carcinoma (NPA) cells and 293 cells did not seem to alter the cellular proliferation rate.…”

Section: Discussionmentioning

confidence: 69%

“…The 5.5 kb NATH transcript harbored an open reading frame encoding a putative 866 amino acid protein, which was homologous to S. cerevisiae NAT1 (Mullen et al, 1989), mouse NARG1 (Sugiura et al, 2001), and to mouse Tbdn-1 (Gendron et al, 2000). The NATH protein contains four tetratricopeptide repeat (TPR) domains in two tandems.…”

Section: Discussionmentioning

confidence: 99%

“…Blast search showed the putative NATH protein to be homologous to Saccharomyces cerevisiae N-acetyltransferase (NAT)1 (Mullen et al, 1989) (854 amino acids, 25% identity). NATH is also homologous to the (partial) sequences of mouse NARG1 (Sugiura et al, 2001) and mouse Tubedown-1 (Tbdn-1) (Gendron et al, 2000) proteins, in the Nterminal and C-terminal parts, respectively (Figure 4). No evidence of alternatively spliced NATH transcripts affecting the open reading frame was detected from either PTC biopsies, or from the papillary carcinoma cell line NPA, in the RT -PCR experiments ( Figure 2c).…”

Section: Nath Is a Novel Gene Up-regulated In Ptcmentioning

confidence: 99%

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NATH, a novel gene overexpressed in papillary thyroid carcinomas

et al. 2002

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 69%

Section: Discussionmentioning

confidence: 99%

Section: Nath Is a Novel Gene Up-regulated In Ptcmentioning

confidence: 99%

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NATH, a novel gene overexpressed in papillary thyroid carcinomas

et al. 2002

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“…Tbdn-1 is expressed at high levels at day 8 of gestation, when yolk sac vasculogenesis and blood island formation is peaking but downregulated at day 10 when formation of larger coalesced vitelline vessels occurs. In adult mouse it is expressed in atrial endocardium, bone morrow and ovarian follicles only (Gendron et al, 2000). Although we observed relatively high expression of Tbdn-1 in spleen, colon, stomach, testis and ovary, it could be of interest to investigate which cell populations within a tumour overexpress it and whether this acetyltransferase has a role in tumour neovascularisation…”

Section: Ga Ca 22mentioning

confidence: 77%

Serological identification and expression analysis of gastric cancer-associated genes

et al. 2002

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Serological identification of tumour antigens by recombinant expression cloning has proved to be an effective strategy for the identification of cancer-associated genes having a relevance to cancer aetiology and progression, and for defining possible targets for immunotherapeutic intervention. In the present study we applied this technique to identify immunogenic proteins for gastric cancer that resulted in isolation of 14 distinct serum-reactive antigens. In order to evaluate their role in tumourigenesis and assess the immunogenicity of the identified antigens, we characterised each cDNA clone by DNA sequence analysis, mRNA tissue distribution, comparison of mRNA levels in cancerous and adjacent non-cancerous tissues and the frequency of antibody responses in allogeneic patient and control sera. Previously unknown splice variants of TACC1 and an uncharacterised gene Ga50 were identified. The expression of a newly identified TACC1 isoform is restricted to brain and gastric cancer tissues. Comparison of mRNA levels by semi-quantitative RT -PCR revealed a relative overexpression of three genes in cancer tissues, including growth factor granulin and Tbdn-1 -an orthologue of the mouse acetyltransferase gene which is associated with blood vessel development. An unusual DNA polymorphism -a three-nucleotide deletion was found in NUCB2 cDNA but its mRNA level was consistently decreased in gastric tumours compared with that in the adjacent noncancerous tissues. This study has revealed several new gastric cancer candidate genes; additional studies are required to gain a deeper insight into their role in the tumorigenesis and their potential as therapeutic targets.

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An SNF2 factor involved in mammalian development and cellular proliferation

Raabe

Abdurrahman

Behbehani

et al. 2001

Developmental Dynamics

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Members of the SNF2 (Sucrose Non-Fermenter) family of chromatin-remodeling proteins function in processes ranging from DNA repair to transcription to methylation. Using differential display, we recently identified a novel member of the SNF2 family that is highly expressed at the mRNA level in proliferating cells and is down-regulated during apoptosis. We have named this gene PASG (Proliferation-Associated SNF2-like Gene). Northern blot analysis of adult mouse tissues shows PASG to be highly expressed in proliferating organs such as thymus, bone marrow, and testis and absent from nonproliferative tissues such as brain and heart. In situ hybridization analysis of mouse embryos shows that PASG is differentially expressed during development, with highest expression in developing face, limbs, skeletal muscle, heart, and tail. In vitro, PASG expression correlates with a shift from a quiescent to a proliferative state. Mice null for PASG (also known as LSH or Hells) are reported to die perinatally, although the mechanism for lethality is unclear (Geiman and Muegge, 2000). To test the hypothesis that PASG functions in cell proliferation, we compared 5-bromodeoxyuridine (BrdU) incorporation in C33A cells transiently transfected with PASG versus empty vector and found that PASG transfected cells showed a significant decrease in the amount of BrdU incorporation. These findings suggest that PASG plays a role in cell proliferation and may function in the development of multiple cell lineages during murine embryogenesis.

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Tubedown-1, A novel acetyltransferase associated with blood vessel development

Cited by 52 publications

References 47 publications

NATH, a novel gene overexpressed in papillary thyroid carcinomas

NATH, a novel gene overexpressed in papillary thyroid carcinomas

Serological identification and expression analysis of gastric cancer-associated genes

An SNF2 factor involved in mammalian development and cellular proliferation

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