Serological identification and expression analysis of gastric cancer-associated genes

Linē, Aija; Stengrévics, Aivars; Slucka, Zane; Li, G; Jankevics, Eriks; Rees, Robert C.

doi:10.1038/sj.bjc.6600321

Cited by 65 publications

(50 citation statements)

References 38 publications

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“…Granulin, a putative growth factor, was noted to be significantly overexpressed in carcinomas compared with adenomas. Previous studies of glioblastoma and gastric and ovarian cancers have also shown an up-regulation of this gene (27)(28)(29). Of the down-regulated genes, DUSP 1, which has known proliferation and differentiation functions, has been shown previously to be down-regulated in follicular carcinomas (17) and in advanced epithelial ovarian cancer (30).…”

Section: Discussionmentioning

confidence: 99%

Gene Expression Profiling of Differentiated Thyroid Neoplasms

Chevillard¹,

Ugolin²,

Vielh

et al. 2004

Clinical Cancer Research

120

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Purpose: The purpose of this research was to identify novel genes that can be targeted as diagnostic and clinical markers of differentiated thyroid tumors.Experimental Design: Gene expression analysis using microarray platform was performed on 6 pathologically normal thyroid samples and 12 primary follicular and papillary thyroid neoplasms. Microarrays containing probes for 5,760 human full-length cDNAs were used for hybridization with total RNA from normal and tumor thyroid samples labeled with Cy3-dUTP and Cy5-dUTP, respectively. Scanned array images were recorded, and data analysis was performed. Selected sets of differentially expressed genes were analyzed using quantitative real-time reverse transcription-PCR for verification.Results: We identified 155 genes that differentiate histologically normal thyroid tissues from benign and malignant thyroid neoplasms. Of these 75 genes were differentiated between follicular neoplasms (adenoma and carcinoma) and the follicular variant of papillary carcinoma. Purely follicular neoplasms (adenomas and carcinomas) shared many genetic profiles, and only 43 genes were distinctly different between these tumors. Hierarchical cluster analysis also differentiated conventional papillary carcinoma from its follicular variant and follicular tumors. The differentially expressed genes were composed of members of cell differentiation, adhesion, immune response, and proliferation associated pathways. Quantitative real-time reverse transcription-PCR analysis of selected genes corroborated the microarray expression results.Conclusions: Our study show the following: (1) differences in gene expression between tumor and nontumor bearing normal thyroid tissue can be identified, (2) a set of genes differentiate follicular neoplasm from follicular variant of papillary carcinoma, (3) follicular adenoma and carcinoma share many of the differentiated genes, and (4) gene expression differences identify conventional papillary carcinoma from the follicular variant.

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Section: Discussionmentioning

confidence: 99%

Gene Expression Profiling of Differentiated Thyroid Neoplasms

Chevillard¹,

Ugolin²,

Vielh

et al. 2004

Clinical Cancer Research

120

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“…From a cancer standpoint, it is of interest to note that the mouse N-terminal acetyltransferase is implicated in vascular development (33)(34)(35) and therefore may play an active role in angiogenesis. The human orthologue, tubedown-100, also termed NATH or Ga19, has been shown to be implicated in transcription of the osteocalcin gene (36) and is overexpressed in papillary thyroid carcinoma (37) and gastric cancer (38). The role, if any, that ARD1 plays in these processes as a NAT-interacting partner remains unstudied.…”

Section: Discussionmentioning

confidence: 99%

Arrest-defective-1 Protein, an Acetyltransferase, Does Not Alter Stability of Hypoxia-inducible Factor (HIF)-1α and Is Not Induced by Hypoxia or HIF

Bilton

Mazure

Trottier

et al. 2005

Journal of Biological Chemistry

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The hypoxia-inducible factor (HIF) is a key player in a transcriptional pathway that controls the hypoxic response of mammalian cells. Post-translational modification of the ␣ subunit of HIF determines its half-life and activity. Among the multiple reported modifications, acetylation, by an acetyltransferase termed arrest-defective-1 protein (ARD1), has been reported to decrease HIF-1␣ stability and therefore impact on hypoxic gene expression. In contrast, we report that both overexpression and silencing of ARD1 had no impact on the stability of HIF-1␣ or -2␣ and that cells silenced for ARD1 maintained hypoxic nuclear localization of HIF-1␣. In addition, we show that the ARD1 mRNA and protein levels are not regulated by hypoxia in several human tumor cell lines, including cervical adenocarcinoma HeLa cells, fibrosarcoma HT1080 cells, adenovirustransformed human kidney HEK293 cells, and human breast cancer MCF-7 cells. Using two model systems ((a) wild-type and HIF-1␣-null mouse embryo fibroblasts and (b) HeLa cells silenced for HIF-1␣ or -2␣ by RNA interference), we demonstrate that the level of expression of the ARD1 protein is independent of HIF-1␣ and -2␣. We also demonstrate that ARD1 is a stable, predominantly cytoplasmic protein expressed in a broad range of tissues, tumor cell lines, and endothelial cells. Taken together, our findings demonstrate that ARD1 has limited, if any, impact on the HIF signaling pathway.

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“…In breast carcinoma, it has been suggested that NUCB2 may be upregulated by estrogen and serve an important role in increased proliferative, migratory and invasive properties in MCF-7 and SK-BR-3 breast cancer cells (13). It has also been suggested in gastric carcinoma that NUCB2 is a candidate biomarker gene for cancer, which may be identified as a novel potential target for immunotherapeutic treatment (25). Furthermore, a study investigating ovarian epithelial carcinoma in 2013 by Xu et al (24) revealed a notable function of NUCB2/nesfatin-1, where it prevented proliferation in the human ovarian epithelial carcinoma HO-8910 cell line through apoptosis signaling, via the mechanistic target of rapamycin-RhoA/RhoA kinase signaling pathway, which serves an important role in cancer cell proliferation and invasion.…”

Section: Discussionmentioning

confidence: 99%

A novel function of NUCB2 in promoting the development and invasion of renal cell carcinoma

et al. 2017

Oncol Lett

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Abstract. Previous studies have assessed nucleobindin 2 (NUCB2) expression in multiple urological cancer cell lines and detected its effect on renal cancer cell apoptosis. Additionally, certain reports have indicated a novel function of NUCB2 in promoting invasion in renal cancer. The levels of NUCB2 expression in different tumor cell lines were detected by quantitative reverse transcription polymerase chain reaction (RT-qPCR). Human NUCB2 and β-actin (ACTB) cDNA plasmids were inserted into lentivirus plasmids, which were then transfected into 786-O cells. Western blotting and RT-qPCR were then performed to determine the gene expression in NUCB2-knocked down cells. Apoptosis was also examined by flow cytometry subsequent to successful transfection. Finally, a transwell invasion assay was performed to investigate the effects on invasive abilities in renal cancer cells. The RT-qPCR results demonstrated a high expression of NUCB2 in 786-O, ACHN and LNCaP cells, and there was particularly high expression in renal cancer 786-O cells. Following successful transfection, downregulation of NUCB2 facilitated renal carcinoma cell apoptosis, as demonstrated by an increased apoptosis rate in the lenti-NUCB2-KD 786-O cells (13.72±0.84 vs. 3.32±0.10; lenti-NUCB2-KD group vs. negative control). Notably, a significant decreased invasion rate was observed in the NUCB2 knocked-down cells compared with negative control, suggesting an invasion-promoting effect of NUCB2. These results suggested a novel function of NUCB2 in the process of development and invasion in renal cell carcinoma. NUCB2 may be an important prognostic factor and target in the diagnosis and treatment of human renal cancer.

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Serological identification and expression analysis of gastric cancer-associated genes

Cited by 65 publications

References 38 publications

Gene Expression Profiling of Differentiated Thyroid Neoplasms

Gene Expression Profiling of Differentiated Thyroid Neoplasms

Arrest-defective-1 Protein, an Acetyltransferase, Does Not Alter Stability of Hypoxia-inducible Factor (HIF)-1α and Is Not Induced by Hypoxia or HIF

A novel function of NUCB2 in promoting the development and invasion of renal cell carcinoma

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