Tualang Honey Protects Keratinocytes from Ultraviolet Radiation‐Induced Inflammation and DNA Damage<sup>†</sup>

Ahmad, Israr; Jimenez, Hugo; Yaacob, Nik Soriani; Yusuf, Nabiha

doi:10.1111/j.1751-1097.2012.01100.x

Cited by 42 publications

(29 citation statements)

References 32 publications

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“…The anti-inflammatory effect of honey was long established through various studies [14, 16, 23, 29] on non-arid region honey, yet nothing was available on arid region varieties. The research results demonstrated here indicate that arid region honey also possesses anti-inflammatory effects similar to previously studied non-arid honey types.…”

Section: Discussionmentioning

confidence: 99%

Bioactivity of arid region honey: an in vitro study

Hilary

Habib

Souka

et al. 2017

BMC Complement Altern Med

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BackgroundAntioxidant and anti-inflammatory properties of honey have been largely recognized by various studies. Almost all of the potential benefits are associated with polyphenol content. Honey varieties from the arid region are reported to be rich in polyphenols, but data related to its bioactivity in vitro is greatly lacking. This study aimed at establishing the antioxidant and anti-inflammatory properties of arid region honey. Four honey varieties from arid region (H1, H2, H3, and H4) and two popular non-arid region honey (H5 and H6) were tested in vitro in this study.MethodsThe erythrocyte membrane protection effect of honey varieties were measured by hemolysis assay after exposing erythrocytes to a peroxide generator. The subsequent production of MDA (malondialdehyde) content in erythrocytes was measured. Immunomodulatory effect of the honey varieties was tested in prostate cancer cells PC-3 and PBMC (peripheral blood mononuclear cells) by measuring the IL-6 (interleukin 6) and NO (nitric oxide) levels in cell culture supernatant after incubation with the honey varieties. PC-3 cell viability was assessed after incubation with honey varieties for 24 h.ResultsArid region honey exhibited superior erythrocyte membrane protection effect with H4 measuring 1.3 ± 0.042mMTE/g and H2 measuring 1.122 ± 0.018mMTE/g. MDA levels were significantly reduced by honey samples, especially H4 (20.819 ± 0.63 nmol/mg protein). We observed a significant decrease in cell population in PC-3 after 24 h in culture on treatment with honey. A moderate increase in NO levels was observed in both cultures after 24 h at the same time levels of IL-6 were remarkably reduced by honey varieties.ConclusionThe results demonstrate the antioxidant effect of arid region honey due to its erythrocyte membrane protection effect and subsequent lowering of oxidative damage as evident from lower levels of lipid peroxidation byproduct MDA. Arid region honey varieties were as good as non-arid region types at decreasing cell viability of prostate cancer cells. The moderate increase in NO levels in PC-3 and PBMCs were not significant enough to elicit any pro-inflammatory response. However, IL-6 secretion was remarkably reduced by all honey varieties in a comparable level indicating the potential anti-inflammatory property of arid region honey.

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Section: Discussionmentioning

confidence: 99%

Bioactivity of arid region honey: an in vitro study

Hilary

Habib

Souka

et al. 2017

BMC Complement Altern Med

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“…Examples of ROS are singlet oxygen, superoxide, peroxyl, and hydroxyl radicals. These ruthless free radicals originate from endogenous stressors, resulting from natural by-products of cellular metabolism, or exogenous stressors such as UV light, pollutants, drugs, smoke, or radiation [31,32]. As their name suggests, these reactive molecules provoke the deterioration of membranes, lipids, amino acids, and DNA [33].…”

Section: Stingless Bee Honey As Antioxidantmentioning

confidence: 99%

Stingless Bee Honey, the Natural Wound Healer: A Review

Jalil¹,

Kasmuri²,

Hadi³

2017

Skin Pharmacol Physiol

161

138

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Background: The stingless bee is a natural type of bee that exists in almost every continent. The honey produced by this bee has been widely used across time and space. The distinctive feature of this honey is that it is stored naturally in the pot (cerumen), thus contributing to its beneficial properties, especially in the wound healing process. Methods: In this article, several studies on stingless bee honey that pointed out the numerous therapeutic profiles of this honey in terms of its antioxidant, antimicrobial, anti-inflammatory, as well as moisturizing properties are reviewed. All of these therapeutic properties are related to wound healing properties. Results: Antioxidant in stingless bee honey could break the chain of free radicals that cause a detrimental effect to the wounded area. Furthermore, the antimicrobial properties of stingless bee honey could overcome the bacterial contamination and thus improve the healing rate. Moreover, the anti-inflammatory attribute in this honey could protect the tissue from highly toxic inflammatory mediators. The moisturizing properties of the honey could improve wound healing by promoting angiogenesis and oxygen circulation. Conclusion: The application of honey to the wound has been widely used since ancient times. As a result, it is essential to understand the pharmacological mechanism of the honey towards the physiology of the wounded skin in order to optimize the healing rate in the future.

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“…The band density for each test was compared to the control and the bands were analyzed using ImageJ 1.46 software [provided by NIH (http://imagej.nih.gov/ij/)]. The values were normalized to the β-actin band density [7,10,11]. Three independent experiments were performed to evaluate the P2Y 12 expression.…”

Section: Western Blot Analysismentioning

confidence: 99%