Tu1980 Low Mitochondrial Activity in Colon Cancer Cells via Loss of FOXO3 Elevates Intracellular Lipid Droplets (LDs) to Accelerate Proliferation

Heller, Sandra; Cable, Chloe; Penrose, Harrison M.; Crawford, Susan E.; Savkovic, Suzana D.

doi:10.1016/s0016-5085(15)33247-9

Cited by 2 publications

(3 citation statements)

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“…Lipids act as metabolic signal messengers in response to upstream signal networks and intracellular nutritional status. − The excessive accumulation of saturated fatty acids in the cell membrane can lead to lipid toxicity, potentially inducing ER stress, and apoptosis. Unsaturated fatty acids are generally considered to have a protective effect on cells. , …”

Section: Resultsmentioning

confidence: 99%

Phenotype Identification of HeLa Cells Knockout CDK6 Gene Based on Label-Free Raman Imaging

Hou

Zhu

et al. 2022

Anal. Chem.

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Identifying cell phenotypes is essential for understanding the function of biological macromolecules and molecular biology. We developed a noninvasive, label-free, single-cell Raman imaging analysis platform to distinguish between the cell phenotypes of the HeLa cell wild type (WT) and cyclin-dependent kinase 6 (CDK6) gene knockout (KO) type. Via large-scale Raman spectral and imaging analysis, two phenotypes of the HeLa cells were distinguished by their intrinsic biochemical profiles. A significant difference was found between the two cell lines: large lipid droplets formed in the knockout HeLa cells but were not observed in the WT cells, which was confirmed by Oil Red O staining. The band ratio of the Raman spectrum of saturated/unsaturated fatty acids was identified as the Raman spectral marker for HeLa cell WT or gene knockout type differentiation. The interaction between organelles involved in lipid metabolism was revealed by Raman imaging and Lorentz fitting, where the distribution intensity of the mitochondria and the endoplasmic reticulum membrane decreased. At the same time, lysosomes increased after the CDK6 gene knockout. The parameters obtained from Raman spectroscopy are based on hierarchical cluster analysis and one-way ANOVA, enabling highly accurate cell classification.

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Section: Resultsmentioning

confidence: 99%

Phenotype Identification of HeLa Cells Knockout CDK6 Gene Based on Label-Free Raman Imaging

Hou

Zhu

et al. 2022

Anal. Chem.

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“…For instance, lipolysis of LD-derived fatty acids in mammalian cells occurs in mitochondria through β-oxidation . The role of mitochondria and LDs in cellular stress and metabolic homeostasis is critical and has also been increasingly implicated in many diseases like diabetes and hepatoma . However, the exceptional function of mitochondria largely relies on the mitochondrial pH.…”

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confidence: 99%

“…6 The role of mitochondria and LDs in cellular stress and metabolic homeostasis is critical and has also been increasingly implicated in many diseases like diabetes 7 and hepatoma. 8 However, the exceptional function of mitochondria largely relies on the mitochondrial pH. Under normal physiological conditions, mitochondria maintain an alkaline matrix (pH ∼ 8), reflecting proton extrusion across the inner membrane via the respiratory electron transport chain.…”

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confidence: 99%

pH-Dominated Selective Imaging of Lipid Droplets and Mitochondria via a Polarity-Reversible Ratiometric Fluorescent Probe

Bai

Yang

et al. 2022

Anal. Chem.

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Elucidating the intrinsic relationship between mitochondrial pH (pH m ) fluctuation and lipid droplets (LDs) formation is vital in cell physiology. The development of small-molecular fluorescent probes for discrimination and simultaneous visualization of pH m fluctuation toward LDs has not yet been reported. In this work, utilizing pH-driven polarityreversible hemicyanine and rhodamine derivatives, a multifunctional fluorescent probe is developed for selectively identifying mitochondria and LDs under specific pH values via dual-emission channels. This rapidresponse probe, Hcy-Rh, has two distinct chemical structures under acidic and alkaline circumstances. In acidic conditions, Hcy-Rh exhibits good hydrophilicity that can target mitochondria and display an intense red fluorescence. Conversely, the probe becomes lipophilic under weakly alkaline conditions and targets LDs, showing a strong blue emission. In this manner, Hcy-Rh can selectively label mitochondria and LDs, exhibiting red and blue fluorescence, respectively. Moreover, this ratiometric probe is applied to map pH m changes in living cells under the stimulus with FCCP, NAC, and H 2 O 2 . The interplay of LD− mitochondria under oleic acid treatment and starvation-induced autophagy has been studied using this probe at different pH values. In a word, Hcy-Rh is a potential candidate for further exploring mitochondria−LD interaction mechanisms under pH m fluctuation. Moreover, the polarity-dependent strategy is valuable for designing other functional biological probes in imaging multiple organelles.

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Tu1980 Low Mitochondrial Activity in Colon Cancer Cells via Loss of FOXO3 Elevates Intracellular Lipid Droplets (LDs) to Accelerate Proliferation

Cited by 2 publications

References 0 publications

Phenotype Identification of HeLa Cells Knockout CDK6 Gene Based on Label-Free Raman Imaging

Phenotype Identification of HeLa Cells Knockout CDK6 Gene Based on Label-Free Raman Imaging

pH-Dominated Selective Imaging of Lipid Droplets and Mitochondria via a Polarity-Reversible Ratiometric Fluorescent Probe

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