Tsukuba High- and Low-Emotional strains of rats (Rattus norvegicus): An overview

Fujita, Osamu; Annen, Yasumasa; Kitaoka, Akiyoshi

doi:10.1007/bf01067540

Cited by 53 publications

(22 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This finding indicates that rats from the LAB line, which have been shown to display a higher activity than HAB rats in an open field (Liebsch et al 1998), do not differ generally in their spontaneous home cage locomotor activity. This is in line with findings in the THE/TLE strains (Fujita et al 1994) and in Roman highand low-avoidance rats that also display differential activity in an open field (Gentsch et al 1981), but only a trend toward higher nighttime activity in high avoidance rats when measured telemetrically in the home cage (Meerlo et al 1997). It also supports the concept of "reactive" versus "spontaneous" locomotor activity (Courvoisier et al 1996;Gentsch et al 1991).…”

supporting

confidence: 77%

Behavioral, Physiological, and Neuroendocrine Stress Responses and Differential Sensitivity to Diazepam in Two Wistar Rat Lines Selectively Bred for High- and Low-Anxiety–Related Behavior

Liebsch

Linthorst

Neumann

et al. 1998

Neuropsychopharmacology

150

View full text Add to dashboard Cite

Two Wistar rat lines, selectively bred for high-anxietyrelated behavior (HAB) and low-anxiety-related behavior (LAB) in the elevated plus-maze test, were tested for the susceptibility of their behavioral characteristics to anxiolytic treatment and for their endocrine and physiological reactivity to different stressors. Injection of 1mg/kg diazepam failed to affect line differences in coping strategy but resulted in a marked (20-fold) Genetic factors contribute to the high interindividual variability in type and/or intensity of the behavioral, neuroendocrine, and physiological stress responses observed in various species, including humans and laboratory rats (Boissy 1995;Clément et al. 1997;Cools et al. 1990;Curé and Rolinat 1992;Prasad et al. 1996;Spanagel et al. 1995;Tesser 1993;Zuckerman 1993). This fact has been used as the basis for selective bidirectional breeding programs from which strains and lines differing in their behavioral and/or neuroendocrine reFrom the Max Planck Institute of Psychiatry, Munich, Germany. Address correspondence to: Dr. R. Landgraf, Max Planck Institute of Psychiatry, Kraepelinstr. 2, 80804 Munich, Germany.Received January 15, 1997; revised March 25, 1998; accepted April 16, 1998. 382 G. Liebsch et al. N EUROPSYCHOPHARMACOLOGY 1998 -VOL . 19 , NO . 5 sponses to environmental challenges have emerged (Brush 1991;Castanon and Mormède 1994;Cools et al. 1993;Gentsch et al. 1988;Walker et al. 1992). Recently, we reported the establishment of two Wistar rat lines selectively bred for differing behavioral performances in the elevated plus-maze test, now termed high-anxiety-related behavior (HAB) and low-anxiety-related behavior (LAB) lines, respectively ). This animal model of inborn anxiety provides the advantage of avoiding conditioned stimuli that by themselves are likely to trigger behavioral, neuroendocrine, and physiological alterations. Interestingly, the HAB and LAB animals also prefer different coping strategies in a forced swim test ), pointing toward a link between anxiety and coping with stressful situations.The elevated plus-maze test is based on the creation of a conflict between the exploratory drive of a rat and its innate fear of open, exposed areas. It is one of the most widely used nonconditioned animal models of anxiety and has been extensively validated pharmacologically and ethologically (Dawson and Tricklebank 1995;Pellow 1986;Pich et al. 1993). However, various clinically effective anxiolytic substances often yield contradictory results when administered to "normal" unstressed rats in this test (Griebel 1995;Rodgers et al. 1997), indicating that-in parallel to the situation in humans (Green and Hodges 1991; Lader 1991)-the efficacy of an anxiolytic may depend upon the animal's basal level of anxiety. To test this prediction and to characterize further our breeding lines, in the first experiment, male rats from the HAB and LAB lines were injected with a classical anxiolytic, diazepam, and submitted to a plus-maze test and a forced swim test to assess the e...

show abstract

supporting

confidence: 77%

Behavioral, Physiological, and Neuroendocrine Stress Responses and Differential Sensitivity to Diazepam in Two Wistar Rat Lines Selectively Bred for High- and Low-Anxiety–Related Behavior

Liebsch

Linthorst

Neumann

et al. 1998

Neuropsychopharmacology

150

View full text Add to dashboard Cite

show abstract

“…Tsukuba High Emotional (THE) and Tsukuba Low Emotional (TLE) strains of rats have been congenitally bred for use in studies of emotionality [3,4]. These strains have been obtained by bidirectional selective breeding of Wistar rats for high (TLE) and low (THE) activity in the runway test.…”

Section: Introductionmentioning

confidence: 99%

“…These strains have been obtained by bidirectional selective breeding of Wistar rats for high (TLE) and low (THE) activity in the runway test. The runway consists of a dark starting box with a small exit and a bright straight runway, which is divided into five sections (25 cm each) in which locomotive activity is recorded [3]. This criterion is based on the supposition that suppression of locomotive activity, primarily represented by "freezing" in a novel situation and/or in the starting box, reflects an emotional reaction thought to be an expression of aroused fear or anxiety in rats.…”

Section: Introductionmentioning

confidence: 99%

Ultrasonic Vocalization Responses in Genetically High- and Low-Emotional Rats

et al. 2001

View full text Add to dashboard Cite

Tsukuba High Emotional (THE) and Tsukuba Low Emotional (TLE) strains of rats have been congenitally bred for use in studies of emotionality. The current study investigated THE and TLE strain differences in the footshock-induced ultrasonic vocalization responses of adult male rats. Ultrasonic vocalization response inducibility (ratio of rats emitting ultrasounds) and vocalization activity in THE rats were statistically higher than in TLE rats. We next examined the causal relation between the ultrasonic vocalizations and the activation of the pituitary-adrenocortical axis in response to footshocks in these two strains of rats. Rats were sorted into two groups, vocalizing and non-vocalizing, after being exposed to the shock regimen daily for 5 successive days. Basal plasma concentrations of ACTH and corticosterone were not different between THE and TLE rats. After receiving footshocks, significant increases in plasma concentrations of ACTH and corticosterone were induced in both strains of rats. These increases in plasma concentrations of ACTH and corticosterone were significantly higher in THE than in TLE rats. However, in vocalizing and non-vocalizing rats of both strains, no statistical differences in plasma concentrations of ACTH and corticosterone were observed after footshocks. These findings suggest that the high emotionality of the adult male rats was reflected in the emission of ultrasounds, and that the emission of ultrasonic vocalizations might not be related to the activation of the pituitary-adrenocortical axis.

show abstract

“…For example, Tsukuba [29], HAB/LAB [30] and Roman [63] animals did not exhibit any motor differences when measured under basal conditions in their home cage using a radiotelemetric system. Therefore, the motor effect observed in these genetic models of anxiety is not associated with general spontaneous locomotor activity but is a reaction to a possible threatening situation.…”

Section: Problem Of Locomotor Activitymentioning

confidence: 99%

“…Most initial research with these strains has been performed in the strain's 1 Floripa is the short name for the city of Florianópolis. country of origin, Japan, and a review with a large amount of physiological and behavioral data was published by Fujita et al [29].…”

Section: Tsukuba High and Low Emotional Ratsmentioning

confidence: 99%

Behavioral evaluation of eight rat lines selected for high and low anxiety-related responses

Gomes

Hassan

Maisonnette

et al. 2013

Behavioural Brain Research

View full text Add to dashboard Cite

evaluation of eight rat lines selected for high and low anxiety-related responses" (2013 • Genetic models are powerful tools to help understand anxiety disorders.• In order to determine the extent to which multiple anxiety traits generalize we compared eight genetic lines of rats selected for single high or low emotional responses.• We find many behavioral traits generalize across different animal lines selected for a single trait. a b s t r a c t Anxiety traits can be stable and permanent characteristics of an individual across time that is less susceptible of influences by a particular situation. One way to study trait anxiety in an experimental context is through the use of rat lines, selected according to contrasting phenotypes of fear and anxiety. It is not clear whether the behavioral differences between two contrasting rat lines in one given anxiety test are also present in others paradigms of state anxiety. Here, we examine the extent to which multiple anxiety traits generalize across selected animal lines originally selected for a single anxiety trait. We review the behavioral results available in the literature of eight rat genetic models of trait anxiety -namely Maudsley Reactive and Non-reactive rats, Floripa H and L rats, Tsukuba High and Low Emotional rats, High and Low Anxiety-related rats, High and Low Ultrasonic Vocalization rats, Roman High and Low Avoidance rats, Syracuse High and Low Avoidance rats, and Carioca High and Low Conditioned Freezing rats -across 11 behavioral paradigms of innate anxiety or aversive learning frequently used in the experimental setting. We observed both convergence and divergence of behavioral responses in these selected lines across the 11 paradigms. We find that predisposition for specific anxiety traits will usually be generalized to other anxiety provoking stimuli. However this generalization is not observed across all genetic models indicating some unique trait and state interactions. Genetic models of enhanced-anxiety related responses are beginning to help define how anxiety can manifest differently depending on the underlying traits and the current environmentally induced state. a r t i c l e i n f o

show abstract

Tsukuba High- and Low-Emotional strains of rats (Rattus norvegicus): An overview

Cited by 53 publications

References 35 publications

Behavioral, Physiological, and Neuroendocrine Stress Responses and Differential Sensitivity to Diazepam in Two Wistar Rat Lines Selectively Bred for High- and Low-Anxiety–Related Behavior

Behavioral, Physiological, and Neuroendocrine Stress Responses and Differential Sensitivity to Diazepam in Two Wistar Rat Lines Selectively Bred for High- and Low-Anxiety–Related Behavior

Ultrasonic Vocalization Responses in Genetically High- and Low-Emotional Rats

Behavioral evaluation of eight rat lines selected for high and low anxiety-related responses

Contact Info

Product

Resources

About