Tryptophan-tagged peptide from serine threonine-protein kinase of Channa striatus improves antioxidant defence in L6 myotubes and attenuates caspase 3–dependent apoptotic response in zebrafish larvae

Cell Biology International

Issac

Saraswathi

et al. 2021

Self Cite

This study investigates the antioxidant and antidiabetic activity of the WL15 peptide derived from Channa striatus on regulating the antioxidant property in the rat skeletal muscle cell line (L6) and enhancing glucose uptake via glucose metabolism. Increased oxidative stress plays a major role in the development of diabetes and its complications.Strategies are needed to mitigate the oxidative stress that can reduce these pathogenic processes. Our results showed that with treatment with WL15 peptide, the reactive oxygen species significantly decreased in L6 myotubes in a dose-dependent manner, and increased antioxidant enzymes help to prevent the formation of lipid peroxidation in L6 myotubes. The cytotoxicity of WL15 is evaluated in the L6 cells and found to be noncytotoxic at the tested concentration. Also, for the analysis of glucose uptake activity in L6 cells, the 2-(N-[7-nitrobenz-2-oxa-1,3-diazol-4-yl]amino)-2-deoxy-D-glucose assay was performed in the presence of wortmannin and genistein inhibitors. WL15 demonstrated antidiabetic activities through a dose-dependent increase in glucose uptake (64%) and glycogen storage (7.8 mM). The optimal concentration for the maximum activity was found to be 50 µM. In addition, studies of gene expression in L6 myotubes demonstrated upregulation of antioxidant genes and genes involved in the pathway of insulin signaling.In cell-based assays, WL15 peptide decreased intracellular reactive oxygen species levels and demonstrated insulin mimic activity by enhancing the primary genes involved in the insulin signaling pathway by increased glucose uptake indicating that glucose transporter type 4 (GLUT4) is regulated from the intracellular pool to the plasma membrane.

Section: Cell Ell B Biology Iology I International Nternationalmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Deteriorating insulin resistance due to WL15 peptide from cysteine and glycine‐rich protein 2 in high glucose‐induced rat skeletal muscle L6 cells

Cell Biology International

Issac

Saraswathi

et al. 2021

Self Cite

“…Eggs were collected after 30 min and incubated at 26 ± 1°C in a 12‐well plate. The collected embryos were observed under the microscope to distinguish the fertilized and unfertilized embryos; for further experiments, the embryos fertilized with normal morphology have been used (Dambal et al, 2017; Issac et al, 2021).…”

Section: Methodsmentioning

confidence: 99%

Anti‐inflammatory role demonstrated both in vitro and in vivo models using nonsteroidal tetranortriterpenoid, Nimbin (N1) and its analogs (N2 and N3) that alleviate the domestication of alternative medicine

Sudhakaran

Prathap

Cell Biology International

et al. 2022

Self Cite

Human health may benefit from the study of natural compounds and phytoconstituents that can protect from inflammation. We investigated Nimbin (N1), a member of the ring C Seco-tetranortriterpenoids family, and its semi-natural analog deacetyl Nimbin namely N2 and N3 for their anti-inflammatory properties. As key findings, N1, N2, and N3 were able to improve wound healing by cell proliferation in a period of 24 h and were able to reduce the reactive oxygen species (ROS) production in Madin-Darby Canine Kidney cells which were screened using dichlorodihydro fluorescein diacetate (DCF-DA) staining. When the zebrafish larvae were subjected to DCF-DA assay N1, N2, and N3 were able to substantially reduce the ROS levels in a dose-dependent manner. In zebrafish larvae, the cell death indicates the fluorescent intensity due to acridine orange staining that was found to be dramatically decreasing upon the treatment of N1, N2, and N3. The cell membrane lipid peroxidation levels were also reduced in a dose-dependent manner upon the treatment of Nimbin and its analogs indicating lesser blue fluorescent levels. Among the Nimbin and its analogs, N2 was subjected to have better activity. To confirm the activity of N1, N2, and N3, in silico characterization was performed using Density functional theory and molecular docking. As a result, N2 exhibited the lowest

“…Chemical shifts were recorded in parts per million (ppm, δ) relative to chloroform (δ = 7.26, singlet). 1 H-NMR splitting patterns are designated as singlet (s), doublet (d), triplet (t), quartet (q), doublet of doublets (dd), multiplet, (m), broad singlet (bs) and etc. Carbon nuclear magnetic resonance ( 13 C-NMR) spectra were recorded on a Bruker BBFO (126 MHz) spectrometer.…”

Section: Nmr Hr-ms and Melting Pointmentioning

confidence: 99%

“…Natural products and their semisynthetic analogs continue to play a dominant role in identifying and developing therapeutically important lead compounds and managing several upregulated events in a diseased situation such as oxidative stress. [1,2] Natural products with antioxidant properties derived from regular food consumption give multifactorial benefits for human health and from ancient times, they are powerful tools to combat various diseases. Ginger, a classic food that is consumed all over the world exhibit a wide variety of biological activities by modulating several molecular targets.…”

Section: Introductionmentioning

confidence: 99%

6‐Gingerol and Semisynthetic 6‐Gingerdione Counteract Oxidative Stress Induced by ROS in Zebrafish

Manjunathan

Chemistry & Biodiversity

Arokiaraj

et al. 2021

Self Cite

6-Gingerol (1) is one of the major components in ginger and developing new synthetic methodologies could bring semisynthetic analogs with improved therapeutic properties. Towards this, multigram scale isolation of 6gingerol with excellent purity was optimized using a simple and robust extraction, followed by column purification. Synthesis of 6-gingerdione, 7 from 6-gingerol was then achieved through selective -OTBDMS protection, DMP oxidation and deprotection reaction sequence for the first time. Compounds 1, 7 and 8 (dehydrozingerone) exhibited excellent cell-free antioxidant properties in DPPH, ABTS, superoxide radical scavenging assay and H 2 O 2 assay at 10-50 μM concentrations. The hemolytic study suggests that up to 50 μM, all three compounds did not exhibit toxicity to human erythrocytes. When H 2 O 2 treated zebrafish larvae groups (96hpf) were exposed to compounds 1, 7 and 8, it increases the SOD (19, 19.1 and 18.7 U/mg protein), CAT (18.1, 16.5,and 15.8 μmol/mg levels and decreases the lipid peroxidation level (13, 15 and 18 nmol/mg protein), respectively. In vivo ROS levels and degree of cell death were studied using DCFDA and Acridine orange assays. Compounds 1, 7 and 8 decreases the ROS and cell death level significantly. Taken together, compounds 1, 7 and 8 exhibit excellent antioxidant properties, counteract H 2 O 2 induced oxidative stress, reduces cell death in zebrafish larvae.