Tryptic and chymotryptic proteases released by larvae of the blowfly, lucilia cuprina

Sandeman, R.M.; Feehan, J.P.; Chandler, Rosemary; Bowles, Vernon M

doi:10.1016/0020-7519(90)90044-n

Cited by 45 publications

(16 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Final concentration in the reaction was 0.1 mM. N-Suc-AAPL-pna (Sandeman et al, 1990) was dissolved in 25% DMF/75% of 100 mM Tris pH 8.0, to a 2.5% final concentration of DMF in the reaction. Final concentration in the reaction was 1 mM.…”

Section: Protease and Inhibition Assaysmentioning

confidence: 99%

Mating-increases trypsin in female Drosophila hemolymph

Pilpel

Nezer

Applebaum

et al. 2008

Insect Biochemistry and Molecular Biology

View full text Add to dashboard Cite

Section: Protease and Inhibition Assaysmentioning

confidence: 99%

Mating-increases trypsin in female Drosophila hemolymph

Pilpel

Nezer

Applebaum

et al. 2008

Insect Biochemistry and Molecular Biology

View full text Add to dashboard Cite

“…The first hours after larval hatching are known to be very important for the establishment of parasitism, because it is during this period that the first contact occurs between the larvae and the environment in which they will become established, feed and develop. Thus, it can be assumed that the proteases produced by the larvae of several flies that cause myiasis are involved in processes of digestion of their hosts' tissues, which would facilitate parasite migration and nutrition , Sandeman et al 1990, Casu et al 1996, Young et al 1996, Brant et al 2010.…”

Section: Discussionmentioning

confidence: 99%

“…Studies carried out with Lucilia cuprina have shown that the E/SP contain proteases that seem to be involved in both larval nutrition and the pathogenesis of the lesion caused by them (Bowles et al 1989, Sandeman et al 1990, Casu et al 1996. Young et al (1996) characterized the E/ SP involved in the initial phase of parasitism by L. cuprina and observed that the number of molecules produced varied dramatically over short intervals, but with the predominance of serine-proteases-type enzymes.…”

Section: Introductionmentioning

confidence: 99%

Proteolytic activity of excretory/secretory products of Cochliomyia hominivorax larvae (Diptera: Calliphoridae)

et al. 2016

View full text Add to dashboard Cite

The protein profiles and proteolytic activity of the excretory secretory products (E/SP) of the first (L1), second (L2) and third (L3) larval stages of Cochliomyia hominivorax were studied in the laboratory. Analysis on the E/SP protein profile was carried out using polyacrylamide gel containing sodium dodecyl sulfate (SDS-PAGE). The E/SP of each larval stage (L1, L2 and L3) treated with protease inhibitors, containing 30μg, 40μg and 50μg of protein, was applied to the 10% polyacrylamide gel. The proteolytic activity of the crude E/SP was analyzed in gels copolymerized with gelatin and by colorimetric assays using azocasein as a substrate, with the characterization of the proteases using synthetic inhibitors. Different protein profiles were observed for the larval instars, with L1 presenting the most complex profile. Nevertheless, various protein bands were observed that were common to all the larval instars. The E/SP of all the instars showed proteolytic activity on gelatin, evidenced by proteolysis zones, predominantly with apparently higher molecular masses in L1, while for L2 and L3 the proteolysis zones could also be observed in regions with lower masses. Tests with protease inhibitors using gelatin as substrate showed that the E/SP of larvae were mainly composed of serine proteases. Additionally, inhibition was observed in L2 E/SP treated previously with EDTA, an inhibitor of metalloproteases. The assays with azocasein revealed a gradual increase of proteolytic activity on this substrate with larval development progress, with the strongest inhibitions being observed after treatments with 3,4-dichloroisocoumarin (DCI) for E/SP of L1, L2 and L3. These results suggest that C. hominivorax larvae produce different proteases, a fact that can be related to the parasite's vital processes for survival, such as penetration into the host's tissues and nutrition during the larval stage.

show abstract

“…Antibody titres and specificities were analysed against two different antigen mixtures, LESP and L 1 H rather than a single L. cuprina extract. LESP is an antigen preparation from second and third instar larvae (Sandeman et al 1990) and was used in the skin inflammation tests , thus the LESP antibody studies examined the role of anti-LESP antibody in the skin inflammatory response to this antigen and resistance to fleece rot and body strike. L 1 H is a first instar larval homogenate prepared according to the method of Bowles, Carnegie & Sandeman (1988) and contains not only LESP proteins but also many other L. cuprina proteins.…”

Section: Larval Productsmentioning

confidence: 99%

Antibody responses to Lucilia cuprina in sheep selected for resistance or susceptibility to L. cuprina

O'Meara

Nesa

Sandeman

1997

Parasite Immunology

View full text Add to dashboard Cite

Sheep bred for resistance (R) or susceptibility (S) to fleece rot and myiasis (blowfly strike) have been shown to differ in inflammatory response to intradermal administration of blowfly (Lucilia cuprina) antigens and artificial challenge. The current paper describes analysis of antibody responses to L. cuprina antigens in the R and S animals. Serum antibody titres and specificities to larval antigens were examined and the specificity of wound exudate antibodies was also investigated in animals artificially challenged with L. cuprina. Titres of L. cuprina specific serum IgA, IgM, IgG2 and IgG1 were measured by ELISA, while specificities were examined on two-dimensional immunoblots of larval homogenates. Exposure to L. cuprina stimulated the production of specific antibody in both R and S animals, however antibody titres did not differ between the R and S animals. There was large variation in antibody specificity between individual animals and some L. cuprina proteins appear to be more frequently recognized by sera from either resistant or susceptible animals, however the recognition of a specific protein could not be solely attributed to the resistance status of the animal. It appears that resistance in these animals may be independent of serum antibody and is likely to be an innate response. Despite high levels of IgG in wound exudates, this antibody recognized few antigens in comparison with serum from the same animal, suggesting that exudate contains little functional antibody in comparison to serum.

show abstract

Tryptic and chymotryptic proteases released by larvae of the blowfly, lucilia cuprina

Cited by 45 publications

References 13 publications

Mating-increases trypsin in female Drosophila hemolymph

Mating-increases trypsin in female Drosophila hemolymph

Proteolytic activity of excretory/secretory products of Cochliomyia hominivorax larvae (Diptera: Calliphoridae)

Antibody responses to Lucilia cuprina in sheep selected for resistance or susceptibility to L. cuprina

Contact Info

Product

Resources

About