2009
DOI: 10.1099/mic.0.032805-0
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Trypsin-specific inhibitors from the basidiomycete Clitocybe nebularis with regulatory and defensive functions

Abstract: We have isolated serine protease inhibitors from the basidiomycete Clitocybe nebularis, CnSPIs, using trypsin affinity chromatography. Full-length gene and cDNA sequences were determined for one of them, named cnispin, and the recombinant protein was expressed in Escherichia coli at high yield. The primary structure and biochemical properties of cnispin are very similar to those of the Lentinus edodes serine protease inhibitor, until now the only member of the I66 family of protease inhibitors in the MEROPS cl… Show more

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Cited by 39 publications
(45 citation statements)
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References 42 publications
(56 reference statements)
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“…Natural isolates of cnispin also show entomotoxic activity against D. melanogaster, albeit lower than that for cospin (Avanzo et al 2009), validating the entomotoxic potential of the I66 family. Furthermore, cospin showed no toxicity against the nematode C. elegans and amoebozoon A. castellanii.…”
Section: Mycospins Fungal Inhibitors Of Serine Proteasesmentioning
confidence: 69%
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“…Natural isolates of cnispin also show entomotoxic activity against D. melanogaster, albeit lower than that for cospin (Avanzo et al 2009), validating the entomotoxic potential of the I66 family. Furthermore, cospin showed no toxicity against the nematode C. elegans and amoebozoon A. castellanii.…”
Section: Mycospins Fungal Inhibitors Of Serine Proteasesmentioning
confidence: 69%
“…A biological role for cospin in the defense of fruiting bodies against Drosophilidae is further corroborated by the absence of trypsin-like protease genes in the C. cinerea genome and by the fact that serine proteases are the predominant digestive proteolytic enzymes in dipterans Terra and Ferreira 2005). In addition to the defensive role directed against exogenous serine proteases, there is some evidence that cnispin and cospin could have a dual role, also participating in the regulation of endogenous serine proteases, in fruiting body development and/or resource recycling (Avanzo et al 2009;). …”
Section: Mycospins Fungal Inhibitors Of Serine Proteasesmentioning
confidence: 92%
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