Trypsin inhibitor fromLeucaena leucocephalaseeds delays and disrupts the development ofAedes aegypti,a multiple-disease vector

Abstract

LTI is a promising new tool to control critical points of Ae. aegypti development. © 2016 Society of Chemical Industry.

First insights into insecticidal activity against Aedes aegypti and partial biochemical characterization of a novel low molecular mass chymotrypsin‐trypsin inhibitor purified from Lonchocarpus sericeus seeds

“…After 96 h of treatment, larvae were observed visually, and protease inhibitor (BBI and LsCTI)‐treated larvae (Figure C and D) were found to be smaller and to have underdeveloped external structures, such as the cuticle and siphon, compared with the control (Figure A) and LsF5‐treated larvae (Figure B). In agreement with our results, others authors have reported the ability of protease inhibitors, such as LTI and MoFTI, to delay Ae. aegypti larval development.…”

“…Similarly, other protease inhibitors did not affect egg hatchability, such as ClTI and the Moringa oleifera flowers trypsin inhibitor (MoFTI) . In contrast, a protease inhibitor from L. leucocephala inhibited egg hatching by 50% in the same conditions . Other classes of proteins have also been reported to inhibit egg hatchability, such as lectins and cysteine proteases …”

“…To evaluate the effect of LsCTI and its precursor protein fraction (LsF5) on Ae. aegypti development, we used the methodology proposed by Almeida Filho et al . Ten eggs were gently placed into glass tubes containing 5 mL of 0.3 mg mL ‐1 LsCTI (3.38 x 10 −5 m ) or LsF5 solution (0.3 mg mL −1 ), both in distilled water.…”

“…The insecticidal potential of protease inhibitors against different insect classes has been reported; for example, protease inhibitors from Adenanthera pavonina (ApTI), Cassia leiandra (ClTI) and Leucaena leucocephala (LTI) have been reported to have effects on larvae of Ae. aegypti (Diptera), those from Inga vera (IVTI) and Poincianella pyramidalis (PpyTI) have been reported to have effects on Anagasta kuehniella neonates, and a protease inhibitor from Dimorphandra mollis (DMTI‐II) has been reported to have effects on Callosobruchus maculatus (Coleoptera).…”

Trypsin inhibitor from Enterolobium contortisiliquum seeds impairs Aedes aegypti development and enhances the activity of Bacillus thuringiensis toxins

“…Toxicity test followed methodology described by Almeida Filho et al ., 30 with slight modifications. Artemia sp.…”