First insights into insecticidal activity against <i>Aedes aegypti</i> and partial biochemical characterization of a novel low molecular mass chymotrypsin‐trypsin inhibitor purified from <i>Lonchocarpus sericeus</i> seeds

Filho, Luiz Cp Almeida; Tabosa, Pedro M S; Hissa, Denise Cavalcante; Vasconcelos, Ilka M.; Carvalho, Ana Fontenele Urano

doi:10.1002/ps.4812

Cited by 8 publications

(9 citation statements)

References 63 publications

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“…Several neglected diseases are arboviruses, that is, viral diseases transmitted through the saliva of contaminated arthropods [10]. Thus, they spread with worrisome epidemiological speed because of human exposure to the insect vectors [11, 12]. In some cases, a single vector can transmit more than one disease, as is the case of the vector Aedes aegypti that may transmit dengue, Zika, and chikungunya.…”

Section: Introductionmentioning

confidence: 99%

Active Essential Oils and Their Components in Use against Neglected Diseases and Arboviruses

Luna

Scotti

et al. 2019

Oxidative Medicine and Cellular Longevity

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The termneglected diseasesrefers to a group of infections caused by various classes of pathogens, including protozoa, viruses, bacteria, and helminths, most often affecting impoverished populations without adequate sanitation living in close contact with infectious vectors and domestic animals. The fact that these diseases were historically not considered priorities for pharmaceutical companies made the available treatments options obsolete, precarious, outdated, and in some cases nonexistent. The use of plants for medicinal, religious, and cosmetic purposes has a history dating back to the emergence of humanity. One of the principal fractions of chemical substances found in plants are essential oils (EOs). EOs consist of a mixture of volatile and hydrophobic secondary metabolites with marked odors, composed primarily of terpenes and phenylpropanoids. They have great commercial value and were widely used in traditional medicine, by phytotherapy practitioners, and in public health services for the treatment of several conditions, including neglected diseases. In addition to the recognized cytoprotective and antioxidative activities of many of these compounds, larvicidal, insecticidal, and antiparasitic activities have been associated with the induction of oxidative stress in parasites, increasing levels of nitric oxide in the infected host, reducing parasite resistance to reactive oxygen species, and increasing lipid peroxidation, ultimately leading to serious damage to cell membranes. The hydrophobicity of these compounds also allows them to cross the membranes of parasites as well as the blood-brain barrier, collaborating in combat at the second stage of several of these infections. Based on these considerations, the aim of this review was to present an update of the potential of EOs, their fractions, and their chemical constituents, against some neglected diseases, including American and African trypanosomiasis, leishmaniasis, and arboviruses, specially dengue.

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Section: Introductionmentioning

confidence: 99%

Active Essential Oils and Their Components in Use against Neglected Diseases and Arboviruses

Luna

Scotti

et al. 2019

Oxidative Medicine and Cellular Longevity

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“…A trypsin inhibitor from S. mukorossi seeds (SMTI) was purified with a recovery of 10.47% and purification fold of 9.26 which was greater than the trypsin inhibitor purified from Inga vera seeds 11 (yield 7.14%; purification fold 1.36) and Lonchocarpus sericeus seeds 10 (yield 0.88%; purification fold 8.26). SDS-PAGE depicted a molecular mass of ~29 kDa, similar to that of other trypsin inhibitors 12,13 .…”

Section: Discussionmentioning

confidence: 96%

“…In addition, pathogens and predators continuously attack them. To overcome these adverse conditions plants produce toxic substances to reduce insect damage 10 . PIs are among these compounds which have attracted significant attention because of their role in plant defense and possible applications in transgenic plants to enhance resistance to pathogens and other insect pests.…”

Section: Discussionmentioning

confidence: 99%

Exploration of anti-insect potential of trypsin inhibitor purified from seeds of Sapindus mukorossi against Bactrocera cucurbitae

Samiksha

Singh

Kesavan

et al. 2019

Sci Rep

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Peptidase inhibitors (PIs) are defense proteins of plants which are active against gut peptidases of different insects. Sapindus mukorossi was identified as a source of bioactive PIs which could confer resistance against Bactrocera cucurbitae, a most devastating pest of several economically important crops. In the present study, a trypsin inhibitor was purified from mature dry seeds of S. mukorossi and characterized for its biochemical properties as well as its potential for bio control of B. cucurbitae. The purified fractions from RP- HPLC through SDS-PAGE gave an apparent molecular weight of ~29 kDa. S. mukorossi trypsin inhibitor (SMTI) was found to be a non-competitive inhibitor which was active over a broad range of temperature (10–100 °C) and pH (6–11). SMTI when incorporated in artificial diet inhibited the growth and development of B. cucurbitae larvae. Gene expression analysis of trypsin and chymotrypsin genes via qRT-PCR indicated that their mRNA expression was down-regulated while that of other genes namely, Catalase, Elastase, Superoxide Dismutase, Glutathione –S-transferase and Alkaline Phosphatase was up regulated. SMTI also showed deleterious effects against different bacterial strains. The results of this study indicated that S. mukorossi trypsin inhibitor has potential to be used as a bio control agent that can reduce the harm caused by melon fruit fly and other devastating pests.

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“…The inhibitory effect of EcTI in vitro toward Ae. aegypti midgut proteases activity was performed following methodology described by Almeida Filho et al 27 . All tests were performed in triplicate and results were expressed as the mean of three independent assays.…”

Section: Methodsmentioning

confidence: 99%

“…Effect of EcTI on egg hatching of Ae. aegypti was performed according to Almeida Filho et al 27 . Ten eggs were placed into 10 mL test tubes containing 5 mL inhibitor solution (710 μg mL −1 ) in distilled water.…”

Section: Methodsmentioning

confidence: 99%

Trypsin inhibitor from Enterolobium contortisiliquum seeds impairs Aedes aegypti development and enhances the activity of Bacillus thuringiensis toxins

Tabosa

Filho

Franca

et al. 2020

Pest Management Science

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BACKGROUND: Disease vector insects are barriers for human development. The use of synthetic chemicals to control these vectors has caused damage to the environment and contributed to the arising of resistant insect populations. This has led to an increased search for plant-derived molecules with insecticidal activity or that show synergistic effects with known insecticidal substances, such as protease inhibitors. Thus, we aimed to evaluate the effect of Enterolobium contortisiliquum trypsin inhibitor (EcTI) on Aedes aegypti development as well as its effect on insecticidal activity of Bacillus thuringiensis toxins. RESULTS: EcTI showed an apparent molecular mass about of 20 kDa in SDS-PAGE and was able to inhibit in vitro the activity of trypsin and proteases from midgut of Ae. aegypti larvae. EcTI was not able to cause acute toxicity on mosquito larvae even at 1000 ∼g mL −1 , however it promoted a delay in larval development after prolonged exposure. The zymogram results for EcTItreated larvae (from 50 to 200 ∼g mL −1) showed an increase of midgut proteases activity as a larvae defense mechanism, however no changes in the enzyme profile was observed. These same concentrations were able to enhance up to three fold the insecticidal activity of B. thuringiensis toxins without causing toxicity to Artemia sp. nauplii, a non-target organism. CONCLUSIONS: The results offer a novel approach by combining EcTI and B. thuringiensis toxins for combating Ae. aegypti larvae.

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First insights into insecticidal activity against Aedes aegypti and partial biochemical characterization of a novel low molecular mass chymotrypsin‐trypsin inhibitor purified from Lonchocarpus sericeus seeds

Abstract: LsCTI is a novel protease inhibitor with remarkable biochemical characteristics and is a potential tool to control Ae. aegypti development. © 2017 Society of Chemical Industry.

Cited by 8 publications

References 63 publications

Active Essential Oils and Their Components in Use against Neglected Diseases and Arboviruses

Active Essential Oils and Their Components in Use against Neglected Diseases and Arboviruses

Exploration of anti-insect potential of trypsin inhibitor purified from seeds of Sapindus mukorossi against Bactrocera cucurbitae

Trypsin inhibitor from Enterolobium contortisiliquum seeds impairs Aedes aegypti development and enhances the activity of Bacillus thuringiensis toxins

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